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Different wavelengths light to induce physiological changes callus for the biosynthesis of gymnemic acid in Gymnema sylvestre

A callus culture of Gymnema sylvestre was cultivated under different light conditions (blue, white, red and green light). Green-white coloured gymnemic acid containing cell groups were formed on the surface of the callus culture under blue light respectively. The phytohormone kinetin enhanced the gy...

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Main Authors: Ahmed, A.B.A., Rao, A.S., Rao, M.V., Taha, R.M.
Format: Article
Language:English
Published: 2012
Subjects:
QH301 Biology
Online Access:http://eprints.um.edu.my/10717/1/Different_wavelengths_light_to_induce_physiological_changes_callus_for_the_biosynthesis_of_gymnemic_acid_in_Gymnema_sylvestre.PDF
http://eprints.um.edu.my/10717/
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spelling my.um.eprints.107172014-08-06T00:28:26Z http://eprints.um.edu.my/10717/ Different wavelengths light to induce physiological changes callus for the biosynthesis of gymnemic acid in Gymnema sylvestre Ahmed, A.B.A. Rao, A.S. Rao, M.V. Taha, R.M. QH301 Biology A callus culture of Gymnema sylvestre was cultivated under different light conditions (blue, white, red and green light). Green-white coloured gymnemic acid containing cell groups were formed on the surface of the callus culture under blue light respectively. The phytohormone kinetin enhanced the gymnemic acid formation in blue light, but was unable to induce gymnemic acid in darkness. The phytohormone 2,4-D induced the formation gymnemic acid under all light conditions studied. Analysis of the callus growth phase under all treatments revealed that gymnemic acid accumulation was maximum (12.22 mg/g d.w) in the callus during stationary phase of 45 days, grown in MS medium with 2,4-D (1.5 mg/l) and KN 0.5 mg/l under white fluorescent light. Blue light enhanced the gymnemic acid accumulation up to 4.4 fold of that found under white fluorescent light and 2.8 fold of that found in intact leaves. Present findings concluded that blue light can be used as tool for enhancing pentacyclic tri-terpenoids in batch culture of G. sylvestre. 2012 Article PeerReviewed application/pdf en http://eprints.um.edu.my/10717/1/Different_wavelengths_light_to_induce_physiological_changes_callus_for_the_biosynthesis_of_gymnemic_acid_in_Gymnema_sylvestre.PDF Ahmed, A.B.A. and Rao, A.S. and Rao, M.V. and Taha, R.M. (2012) Different wavelengths light to induce physiological changes callus for the biosynthesis of gymnemic acid in Gymnema sylvestre. Agro Food Industry Hi-Tech, 23 (3). pp. 31-34. ISSN 1722-6992
institution Universiti Malaya
building UM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaya
content_source UM Research Repository
url_provider http://eprints.um.edu.my/
language English
topic QH301 Biology
spellingShingle QH301 Biology
Ahmed, A.B.A.
Rao, A.S.
Rao, M.V.
Taha, R.M.
Different wavelengths light to induce physiological changes callus for the biosynthesis of gymnemic acid in Gymnema sylvestre
description A callus culture of Gymnema sylvestre was cultivated under different light conditions (blue, white, red and green light). Green-white coloured gymnemic acid containing cell groups were formed on the surface of the callus culture under blue light respectively. The phytohormone kinetin enhanced the gymnemic acid formation in blue light, but was unable to induce gymnemic acid in darkness. The phytohormone 2,4-D induced the formation gymnemic acid under all light conditions studied. Analysis of the callus growth phase under all treatments revealed that gymnemic acid accumulation was maximum (12.22 mg/g d.w) in the callus during stationary phase of 45 days, grown in MS medium with 2,4-D (1.5 mg/l) and KN 0.5 mg/l under white fluorescent light. Blue light enhanced the gymnemic acid accumulation up to 4.4 fold of that found under white fluorescent light and 2.8 fold of that found in intact leaves. Present findings concluded that blue light can be used as tool for enhancing pentacyclic tri-terpenoids in batch culture of G. sylvestre.
format Article
author Ahmed, A.B.A.
Rao, A.S.
Rao, M.V.
Taha, R.M.
author_facet Ahmed, A.B.A.
Rao, A.S.
Rao, M.V.
Taha, R.M.
author_sort Ahmed, A.B.A.
title Different wavelengths light to induce physiological changes callus for the biosynthesis of gymnemic acid in Gymnema sylvestre
title_short Different wavelengths light to induce physiological changes callus for the biosynthesis of gymnemic acid in Gymnema sylvestre
title_full Different wavelengths light to induce physiological changes callus for the biosynthesis of gymnemic acid in Gymnema sylvestre
title_fullStr Different wavelengths light to induce physiological changes callus for the biosynthesis of gymnemic acid in Gymnema sylvestre
title_full_unstemmed Different wavelengths light to induce physiological changes callus for the biosynthesis of gymnemic acid in Gymnema sylvestre
title_sort different wavelengths light to induce physiological changes callus for the biosynthesis of gymnemic acid in gymnema sylvestre
publishDate 2012
url http://eprints.um.edu.my/10717/1/Different_wavelengths_light_to_induce_physiological_changes_callus_for_the_biosynthesis_of_gymnemic_acid_in_Gymnema_sylvestre.PDF
http://eprints.um.edu.my/10717/
_version_ 1643688869659410432
score 13.211869

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