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Effects of cocktail of four local Malaysian medicinal plants (Phyllanthus spp.) against dengue virus 2

Background: The absence of commercialized vaccines and antiviral agents against dengue has made the disease a major health concern around the world. With the current dengue virus transmission rate and incidences, the development of antiviral drugs is of vital need. The aim of this project was to eva...

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Main Authors: Lee, S.H., Tang, Y.Q., Rathkrishnan, A., Wang, S.M., Ong, K.C., Manikam, R., Payne, B.J., Jaganath, I.B., Sekaran, S.D.
Format: Article
Language:English
Published: BioMed Central 2013
Subjects:
R Medicine
Online Access:http://eprints.um.edu.my/10627/1/Lee-2013-Effects_of_cocktail.pdf
http://eprints.um.edu.my/10627/
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spelling my.um.eprints.106272017-07-14T07:04:00Z http://eprints.um.edu.my/10627/ Effects of cocktail of four local Malaysian medicinal plants (Phyllanthus spp.) against dengue virus 2 Lee, S.H. Tang, Y.Q. Rathkrishnan, A. Wang, S.M. Ong, K.C. Manikam, R. Payne, B.J. Jaganath, I.B. Sekaran, S.D. R Medicine Background: The absence of commercialized vaccines and antiviral agents against dengue has made the disease a major health concern around the world. With the current dengue virus transmission rate and incidences, the development of antiviral drugs is of vital need. The aim of this project was to evaluate the possibility of developing a local medicinal plant, Phyllanthus as an anti-dengue agent. Methods: Cocktail (aqueous and methanolic) extracts were prepared from four species of Phyllanthus (P. amarus, P. niruri, P. urinaria, and P. watsonii) and their polyphenolic compounds were identified via HPLC and LC-MS/MS analysis. MTS assay was then carried out to determine the maximal non-toxic dose (MNTD) of the extracts, followed by screening of the in vitro antiviral activity of aqueous cocktail extracts against DENV2 by means of time-of -addition (pre-, simultaneous and post-) using RT-qPCR. The differentially expressed proteins in the treated and infected cells were analysed with two dimensional gel electrophoresis experiments. Results: Several active compounds including gallic acid, geraniin, syringin, and corilagen have been identified. The MNTD of both aqueous and methanolic extracts on Vero cells were 250.0 mu g/ml and 15.63 mu g/ml respectively. Phyllanthus showed strongest inhibitory activity against DENV2 with more than 90 of virus reduction in simultaneous treatment. Two-dimensional analysis revealed significantly altered levels of thirteen proteins, which were successfully identified by tandem MS (MS/MS). These altered proteins were involved in several biological processes, including viral entry, viral transcription and translation regulations, cytoskeletal assembly, and cellular metabolisms. Conclusions: Phyllanthus could be potentially developed as an anti-DENV agent. BioMed Central 2013 Article PeerReviewed application/pdf en http://eprints.um.edu.my/10627/1/Lee-2013-Effects_of_cocktail.pdf Lee, S.H. and Tang, Y.Q. and Rathkrishnan, A. and Wang, S.M. and Ong, K.C. and Manikam, R. and Payne, B.J. and Jaganath, I.B. and Sekaran, S.D. (2013) Effects of cocktail of four local Malaysian medicinal plants (Phyllanthus spp.) against dengue virus 2. BMC Complementary and Alternative Medicine, 13. ISSN 1472-6882
institution Universiti Malaya
building UM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaya
content_source UM Research Repository
url_provider http://eprints.um.edu.my/
language English
topic R Medicine
spellingShingle R Medicine
Lee, S.H.
Tang, Y.Q.
Rathkrishnan, A.
Wang, S.M.
Ong, K.C.
Manikam, R.
Payne, B.J.
Jaganath, I.B.
Sekaran, S.D.
Effects of cocktail of four local Malaysian medicinal plants (Phyllanthus spp.) against dengue virus 2
description Background: The absence of commercialized vaccines and antiviral agents against dengue has made the disease a major health concern around the world. With the current dengue virus transmission rate and incidences, the development of antiviral drugs is of vital need. The aim of this project was to evaluate the possibility of developing a local medicinal plant, Phyllanthus as an anti-dengue agent. Methods: Cocktail (aqueous and methanolic) extracts were prepared from four species of Phyllanthus (P. amarus, P. niruri, P. urinaria, and P. watsonii) and their polyphenolic compounds were identified via HPLC and LC-MS/MS analysis. MTS assay was then carried out to determine the maximal non-toxic dose (MNTD) of the extracts, followed by screening of the in vitro antiviral activity of aqueous cocktail extracts against DENV2 by means of time-of -addition (pre-, simultaneous and post-) using RT-qPCR. The differentially expressed proteins in the treated and infected cells were analysed with two dimensional gel electrophoresis experiments. Results: Several active compounds including gallic acid, geraniin, syringin, and corilagen have been identified. The MNTD of both aqueous and methanolic extracts on Vero cells were 250.0 mu g/ml and 15.63 mu g/ml respectively. Phyllanthus showed strongest inhibitory activity against DENV2 with more than 90 of virus reduction in simultaneous treatment. Two-dimensional analysis revealed significantly altered levels of thirteen proteins, which were successfully identified by tandem MS (MS/MS). These altered proteins were involved in several biological processes, including viral entry, viral transcription and translation regulations, cytoskeletal assembly, and cellular metabolisms. Conclusions: Phyllanthus could be potentially developed as an anti-DENV agent.
format Article
author Lee, S.H.
Tang, Y.Q.
Rathkrishnan, A.
Wang, S.M.
Ong, K.C.
Manikam, R.
Payne, B.J.
Jaganath, I.B.
Sekaran, S.D.
author_facet Lee, S.H.
Tang, Y.Q.
Rathkrishnan, A.
Wang, S.M.
Ong, K.C.
Manikam, R.
Payne, B.J.
Jaganath, I.B.
Sekaran, S.D.
author_sort Lee, S.H.
title Effects of cocktail of four local Malaysian medicinal plants (Phyllanthus spp.) against dengue virus 2
title_short Effects of cocktail of four local Malaysian medicinal plants (Phyllanthus spp.) against dengue virus 2
title_full Effects of cocktail of four local Malaysian medicinal plants (Phyllanthus spp.) against dengue virus 2
title_fullStr Effects of cocktail of four local Malaysian medicinal plants (Phyllanthus spp.) against dengue virus 2
title_full_unstemmed Effects of cocktail of four local Malaysian medicinal plants (Phyllanthus spp.) against dengue virus 2
title_sort effects of cocktail of four local malaysian medicinal plants (phyllanthus spp.) against dengue virus 2
publisher BioMed Central
publishDate 2013
url http://eprints.um.edu.my/10627/1/Lee-2013-Effects_of_cocktail.pdf
http://eprints.um.edu.my/10627/
_version_ 1643688848911237120
score 13.211869

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