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Optimizing in-house DNA extraction system for the detection of sus scrofa DNA traces in pharma-cosmeceutical product / Hannah Md Mahir

The purpose of this study was to do an optimization of Reagent B in the newly produced in-house DNA extraction kit, for the purpose of reducing the unnecessary consumption of the reagent but still maintaining the efficiency in extracting an optimum purity and yield of DNA. Subsequently, this extract...

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Main Author: Md Mahir, Hannah
Format: Thesis
Language:English
Published: 2007
Subjects:
DNA. Deoxyribonucleic acids
RM Therapeutics. Pharmacology
RS Pharmacy and materia medica
Extraction
Online Access:https://ir.uitm.edu.my/id/eprint/99961/1/99961.PDF
https://ir.uitm.edu.my/id/eprint/99961/
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spelling my.uitm.ir.999612024-08-07T14:15:42Z https://ir.uitm.edu.my/id/eprint/99961/ Optimizing in-house DNA extraction system for the detection of sus scrofa DNA traces in pharma-cosmeceutical product / Hannah Md Mahir Md Mahir, Hannah DNA. Deoxyribonucleic acids RM Therapeutics. Pharmacology RS Pharmacy and materia medica Extraction The purpose of this study was to do an optimization of Reagent B in the newly produced in-house DNA extraction kit, for the purpose of reducing the unnecessary consumption of the reagent but still maintaining the efficiency in extracting an optimum purity and yield of DNA. Subsequently, this extraction kit was tested on several pharma-cosmeceutical products to ensure the existence of porcine DNA traces in the samples. This study was start on by extracting DNA from various samples using the in­ house DNA extraction kit. Then, followed by conventional PCR to compare the DNA from samples with positive control. Consequently, running gel electrophoresis also being employed after the extraction process to visualize the size of DNA acquired and after the PCR to visualize the size of amplicons produced and to verify the specificity of the primer used. In conclusion, the optimum concentration of Reagent B to be employed in the in-house DNA extraction kit is lmg/ml and the optimized in-house extraction kit plus the primer utilized in this study can become a promising tool in clarification of porcine DNA traces in Halal authentication. 2007 Thesis NonPeerReviewed text en https://ir.uitm.edu.my/id/eprint/99961/1/99961.PDF Optimizing in-house DNA extraction system for the detection of sus scrofa DNA traces in pharma-cosmeceutical product / Hannah Md Mahir. (2007) Degree thesis, thesis, Universiti Teknologi MARA (Kampus Puncak Alam).
institution Universiti Teknologi Mara
building Tun Abdul Razak Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Teknologi Mara
content_source UiTM Institutional Repository
url_provider http://ir.uitm.edu.my/
language English
topic DNA. Deoxyribonucleic acids
RM Therapeutics. Pharmacology
RS Pharmacy and materia medica
Extraction
spellingShingle DNA. Deoxyribonucleic acids
RM Therapeutics. Pharmacology
RS Pharmacy and materia medica
Extraction
Md Mahir, Hannah
Optimizing in-house DNA extraction system for the detection of sus scrofa DNA traces in pharma-cosmeceutical product / Hannah Md Mahir
description The purpose of this study was to do an optimization of Reagent B in the newly produced in-house DNA extraction kit, for the purpose of reducing the unnecessary consumption of the reagent but still maintaining the efficiency in extracting an optimum purity and yield of DNA. Subsequently, this extraction kit was tested on several pharma-cosmeceutical products to ensure the existence of porcine DNA traces in the samples. This study was start on by extracting DNA from various samples using the in­ house DNA extraction kit. Then, followed by conventional PCR to compare the DNA from samples with positive control. Consequently, running gel electrophoresis also being employed after the extraction process to visualize the size of DNA acquired and after the PCR to visualize the size of amplicons produced and to verify the specificity of the primer used. In conclusion, the optimum concentration of Reagent B to be employed in the in-house DNA extraction kit is lmg/ml and the optimized in-house extraction kit plus the primer utilized in this study can become a promising tool in clarification of porcine DNA traces in Halal authentication.
format Thesis
author Md Mahir, Hannah
author_facet Md Mahir, Hannah
author_sort Md Mahir, Hannah
title Optimizing in-house DNA extraction system for the detection of sus scrofa DNA traces in pharma-cosmeceutical product / Hannah Md Mahir
title_short Optimizing in-house DNA extraction system for the detection of sus scrofa DNA traces in pharma-cosmeceutical product / Hannah Md Mahir
title_full Optimizing in-house DNA extraction system for the detection of sus scrofa DNA traces in pharma-cosmeceutical product / Hannah Md Mahir
title_fullStr Optimizing in-house DNA extraction system for the detection of sus scrofa DNA traces in pharma-cosmeceutical product / Hannah Md Mahir
title_full_unstemmed Optimizing in-house DNA extraction system for the detection of sus scrofa DNA traces in pharma-cosmeceutical product / Hannah Md Mahir
title_sort optimizing in-house dna extraction system for the detection of sus scrofa dna traces in pharma-cosmeceutical product / hannah md mahir
publishDate 2007
url https://ir.uitm.edu.my/id/eprint/99961/1/99961.PDF
https://ir.uitm.edu.my/id/eprint/99961/
_version_ 1806783733226799104
score 13.211869

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