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Evaluation of the cytotoxicity and genotoxicity of octomeles sumatrana (datistaccae) on human cell lines / Wan Nabihah Wan Razdi

Octomeles Sumatrana (OS), common name, 'Binuang', is one of the traditional medicines used by the Kadazandusun communities in Sabah. Traditionally, it is used as a tonic or to treat stomach ache, bone pain and muscle sprain. The aim of this study was to assess the cytotoxicity and genotoxi...

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Main Author: Wan Razdi, Wan Nabihah
Format: Thesis
Language:English
Published: 2007
Subjects:
RM Therapeutics. Pharmacology
RS Pharmacy and materia medica
Online Access:https://ir.uitm.edu.my/id/eprint/99586/1/99586.PDF
https://ir.uitm.edu.my/id/eprint/99586/
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spelling my.uitm.ir.995862024-08-02T04:08:02Z https://ir.uitm.edu.my/id/eprint/99586/ Evaluation of the cytotoxicity and genotoxicity of octomeles sumatrana (datistaccae) on human cell lines / Wan Nabihah Wan Razdi Wan Razdi, Wan Nabihah RM Therapeutics. Pharmacology RS Pharmacy and materia medica Octomeles Sumatrana (OS), common name, 'Binuang', is one of the traditional medicines used by the Kadazandusun communities in Sabah. Traditionally, it is used as a tonic or to treat stomach ache, bone pain and muscle sprain. The aim of this study was to assess the cytotoxicity and genotoxicity of OS in normal cultured human cell lines as well cancer cell lines. In the cytotoxicity study, the normal cell line used was WRL 68 normal liver cell while the cancer cells were K562 leukemic cell, HepG2 liver cancer cell, MCF- 7 breast cancer cell and HCT-115 colon cancer to represent the different human target organs. Basically, all the cells were cultured in their specified media and after 24 hours incubation, all the cultured cells were treated with different concentrations of OS aqueous extract (0.1 µg/ml to 10000 µg/ml). Following a 24 hour incubation, the viability of the cultured cells was assayed by the MTS assay. The results obtained from the cytotoxicity study showed that OS did not exert the toxic effects against all cell lines. The genotoxicity study was carried out with the concentrations of OS that affected cell viability by 20% only (0.1 µg/ml to 100 µg/ml). The cells used in this study were WRL68 and HepG2. Effect of OS aqueous extract on the DNA in these cells was determined using the comet assay. The results showed that OS was not genotoxic to the cells. In conclusion, OS bark extract was not cytotoxic or genotoxic to human normal and cancerous cell lines. However, further studies are needed to validate these effects in order to ensure the safe use of OS as a traditional medicine. 2007 Thesis NonPeerReviewed text en https://ir.uitm.edu.my/id/eprint/99586/1/99586.PDF Evaluation of the cytotoxicity and genotoxicity of octomeles sumatrana (datistaccae) on human cell lines / Wan Nabihah Wan Razdi. (2007) Degree thesis, thesis, Universiti Teknologi MARA (Kampus Puncak Alam).
institution Universiti Teknologi Mara
building Tun Abdul Razak Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Teknologi Mara
content_source UiTM Institutional Repository
url_provider http://ir.uitm.edu.my/
language English
topic RM Therapeutics. Pharmacology
RS Pharmacy and materia medica
spellingShingle RM Therapeutics. Pharmacology
RS Pharmacy and materia medica
Wan Razdi, Wan Nabihah
Evaluation of the cytotoxicity and genotoxicity of octomeles sumatrana (datistaccae) on human cell lines / Wan Nabihah Wan Razdi
description Octomeles Sumatrana (OS), common name, 'Binuang', is one of the traditional medicines used by the Kadazandusun communities in Sabah. Traditionally, it is used as a tonic or to treat stomach ache, bone pain and muscle sprain. The aim of this study was to assess the cytotoxicity and genotoxicity of OS in normal cultured human cell lines as well cancer cell lines. In the cytotoxicity study, the normal cell line used was WRL 68 normal liver cell while the cancer cells were K562 leukemic cell, HepG2 liver cancer cell, MCF- 7 breast cancer cell and HCT-115 colon cancer to represent the different human target organs. Basically, all the cells were cultured in their specified media and after 24 hours incubation, all the cultured cells were treated with different concentrations of OS aqueous extract (0.1 µg/ml to 10000 µg/ml). Following a 24 hour incubation, the viability of the cultured cells was assayed by the MTS assay. The results obtained from the cytotoxicity study showed that OS did not exert the toxic effects against all cell lines. The genotoxicity study was carried out with the concentrations of OS that affected cell viability by 20% only (0.1 µg/ml to 100 µg/ml). The cells used in this study were WRL68 and HepG2. Effect of OS aqueous extract on the DNA in these cells was determined using the comet assay. The results showed that OS was not genotoxic to the cells. In conclusion, OS bark extract was not cytotoxic or genotoxic to human normal and cancerous cell lines. However, further studies are needed to validate these effects in order to ensure the safe use of OS as a traditional medicine.
format Thesis
author Wan Razdi, Wan Nabihah
author_facet Wan Razdi, Wan Nabihah
author_sort Wan Razdi, Wan Nabihah
title Evaluation of the cytotoxicity and genotoxicity of octomeles sumatrana (datistaccae) on human cell lines / Wan Nabihah Wan Razdi
title_short Evaluation of the cytotoxicity and genotoxicity of octomeles sumatrana (datistaccae) on human cell lines / Wan Nabihah Wan Razdi
title_full Evaluation of the cytotoxicity and genotoxicity of octomeles sumatrana (datistaccae) on human cell lines / Wan Nabihah Wan Razdi
title_fullStr Evaluation of the cytotoxicity and genotoxicity of octomeles sumatrana (datistaccae) on human cell lines / Wan Nabihah Wan Razdi
title_full_unstemmed Evaluation of the cytotoxicity and genotoxicity of octomeles sumatrana (datistaccae) on human cell lines / Wan Nabihah Wan Razdi
title_sort evaluation of the cytotoxicity and genotoxicity of octomeles sumatrana (datistaccae) on human cell lines / wan nabihah wan razdi
publishDate 2007
url https://ir.uitm.edu.my/id/eprint/99586/1/99586.PDF
https://ir.uitm.edu.my/id/eprint/99586/
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score 13.211869

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