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Screening the potential of antagonistic microorganisms as biological control agent for controlling rice blast pathogen, pyricularia oryzae / Muhamad Syafiei Azali

One of the major diseases that attack rice is blast disease, which has caused a great economic loss in rice productions worldwide. This disease is caused by the fungal pathogen known as Pyricularia oryzae. Besides of yield reduction, this disease considered important due to the problem of fungicide...

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Main Author: Azali, Muhamad Syafiei
Format: Thesis
Language:English
Published: 2016
Subjects:
Weeds, parasitic plants, etc.
Online Access:https://ir.uitm.edu.my/id/eprint/99053/1/99053.pdf
https://ir.uitm.edu.my/id/eprint/99053/
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spelling my.uitm.ir.990532024-12-17T06:28:17Z https://ir.uitm.edu.my/id/eprint/99053/ Screening the potential of antagonistic microorganisms as biological control agent for controlling rice blast pathogen, pyricularia oryzae / Muhamad Syafiei Azali Azali, Muhamad Syafiei Weeds, parasitic plants, etc. One of the major diseases that attack rice is blast disease, which has caused a great economic loss in rice productions worldwide. This disease is caused by the fungal pathogen known as Pyricularia oryzae. Besides of yield reduction, this disease considered important due to the problem of fungicide resistance. Fungal pathogen P. oryzae has already developed resistance to several active ingredients of fungicide such as carbendazium and tricyclazole. In order to tackle the problem of fungicide resistance, reduce the application of fungicides in the rice fields is crucial needed. Biological control could be an alternative method for controlling blast disease of rice, which also could reduce the load of chemical fungicides on the rice field. In this study, bacteria and fungi were isolated from asymptomatic rice leaves and stem and were tested for their antagonistic potential against P. oryzae in vitro and in vivo. A dual culture test was conducted as primary screening to evaluate all isolated bacteria and fungi for their potential as biological control of blast disease. Out of 58 bacteria isolates, four isolates showed antagonistic activity against the pathogenic fungus in the dual culture test. Isolate named as B2 (Bacillus cereus) showed the highest percentage of inhibition of radial growth with value of 58.16% as compared to isolate B1 (Bacillus sp.), B3 (Bacillus sp.), and B4 (Bacillus sp.) which PIRG value of 25%, 44.71%, and 54.32% respectively. In culture filtrate test, isolate B2 also shown the highest suppressing effect against the growth of P. oryzae. Secondary metabolite produced by isolate B2 significantly inhibited the growth of the pathogen with 40.58% of PIRG value as compared to the control plate. In addition, a study of isolate B2 on four week old rice seedlings inoculated with P. oryzae also showed a low disease incidence (25%) and percentage of disease severity index as low as 12.5%. Out of 37 fungi that were isolated, none of them showed antagonistic effect against P. oryzae in the dual culture test. 2016 Thesis NonPeerReviewed text en https://ir.uitm.edu.my/id/eprint/99053/1/99053.pdf Screening the potential of antagonistic microorganisms as biological control agent for controlling rice blast pathogen, pyricularia oryzae / Muhamad Syafiei Azali. (2016) Masters thesis, thesis, Universiti Teknologi MARA (UiTM). <http://terminalib.uitm.edu.my/99053.pdf>
institution Universiti Teknologi Mara
building Tun Abdul Razak Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Teknologi Mara
content_source UiTM Institutional Repository
url_provider http://ir.uitm.edu.my/
language English
topic Weeds, parasitic plants, etc.
spellingShingle Weeds, parasitic plants, etc.
Azali, Muhamad Syafiei
Screening the potential of antagonistic microorganisms as biological control agent for controlling rice blast pathogen, pyricularia oryzae / Muhamad Syafiei Azali
description One of the major diseases that attack rice is blast disease, which has caused a great economic loss in rice productions worldwide. This disease is caused by the fungal pathogen known as Pyricularia oryzae. Besides of yield reduction, this disease considered important due to the problem of fungicide resistance. Fungal pathogen P. oryzae has already developed resistance to several active ingredients of fungicide such as carbendazium and tricyclazole. In order to tackle the problem of fungicide resistance, reduce the application of fungicides in the rice fields is crucial needed. Biological control could be an alternative method for controlling blast disease of rice, which also could reduce the load of chemical fungicides on the rice field. In this study, bacteria and fungi were isolated from asymptomatic rice leaves and stem and were tested for their antagonistic potential against P. oryzae in vitro and in vivo. A dual culture test was conducted as primary screening to evaluate all isolated bacteria and fungi for their potential as biological control of blast disease. Out of 58 bacteria isolates, four isolates showed antagonistic activity against the pathogenic fungus in the dual culture test. Isolate named as B2 (Bacillus cereus) showed the highest percentage of inhibition of radial growth with value of 58.16% as compared to isolate B1 (Bacillus sp.), B3 (Bacillus sp.), and B4 (Bacillus sp.) which PIRG value of 25%, 44.71%, and 54.32% respectively. In culture filtrate test, isolate B2 also shown the highest suppressing effect against the growth of P. oryzae. Secondary metabolite produced by isolate B2 significantly inhibited the growth of the pathogen with 40.58% of PIRG value as compared to the control plate. In addition, a study of isolate B2 on four week old rice seedlings inoculated with P. oryzae also showed a low disease incidence (25%) and percentage of disease severity index as low as 12.5%. Out of 37 fungi that were isolated, none of them showed antagonistic effect against P. oryzae in the dual culture test.
format Thesis
author Azali, Muhamad Syafiei
author_facet Azali, Muhamad Syafiei
author_sort Azali, Muhamad Syafiei
title Screening the potential of antagonistic microorganisms as biological control agent for controlling rice blast pathogen, pyricularia oryzae / Muhamad Syafiei Azali
title_short Screening the potential of antagonistic microorganisms as biological control agent for controlling rice blast pathogen, pyricularia oryzae / Muhamad Syafiei Azali
title_full Screening the potential of antagonistic microorganisms as biological control agent for controlling rice blast pathogen, pyricularia oryzae / Muhamad Syafiei Azali
title_fullStr Screening the potential of antagonistic microorganisms as biological control agent for controlling rice blast pathogen, pyricularia oryzae / Muhamad Syafiei Azali
title_full_unstemmed Screening the potential of antagonistic microorganisms as biological control agent for controlling rice blast pathogen, pyricularia oryzae / Muhamad Syafiei Azali
title_sort screening the potential of antagonistic microorganisms as biological control agent for controlling rice blast pathogen, pyricularia oryzae / muhamad syafiei azali
publishDate 2016
url https://ir.uitm.edu.my/id/eprint/99053/1/99053.pdf
https://ir.uitm.edu.my/id/eprint/99053/
_version_ 1818838309293522944
score 13.222552

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