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Design and development of a PCR method for detection of SNP of CYP2B6 / Tengku Aliaa Dayana Tuan Mohd Hashim

Cytochrome P450 286 is an enzyme that involved in the biotransformation of many clinically important drugs. Genetic polymorphisms of the CYP2B6 gene are an important factor that contributes to the inter-individual and inter-ethnic variability in the systemic exposure, therapeutic and toxic responses...

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Main Author: Tuan Mohd Hashim, Tengku Aliaa Dayana
Format: Thesis
Language:English
Published: 2006
Subjects:
RM Therapeutics. Pharmacology
RS Pharmacy and materia medica
Online Access:https://ir.uitm.edu.my/id/eprint/99045/1/99045.PDF
https://ir.uitm.edu.my/id/eprint/99045/
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spelling my.uitm.ir.990452024-07-24T03:07:41Z https://ir.uitm.edu.my/id/eprint/99045/ Design and development of a PCR method for detection of SNP of CYP2B6 / Tengku Aliaa Dayana Tuan Mohd Hashim Tuan Mohd Hashim, Tengku Aliaa Dayana RM Therapeutics. Pharmacology RS Pharmacy and materia medica Cytochrome P450 286 is an enzyme that involved in the biotransformation of many clinically important drugs. Genetic polymorphisms of the CYP2B6 gene are an important factor that contributes to the inter-individual and inter-ethnic variability in the systemic exposure, therapeutic and toxic responses to CYP2B6 substrate drugs. Therefore, it is important to develop a method to identify and determine all of the genetic variants in a CYP2B6 gene that responsible for altered CYP2B6 expression. The aim of this study was to design and develop rapid and simple method for the detection of SNP in the CYP2B6 gene. The multiplex, allele-specific PCR method was used. This method involves two-stage procedures, which were first and second PCR. First PCR was used to amplify 3 exons, which were exon 4, exon 5+6, and exon 9. After that, the products of the first PCR were used as a DNA template and genotyped by allele-specific PCR. The assay detected the following published single nucleotide polymorphisms such as G516T (Gln172His), A785G (Lys262Arg) and C1459T (Arg487Cys). DNA sequencing analysis was performed to further confirmed genotype results obtained from current method. Multiplex PCR with allele-specific genotyping method is simple, reliable, rapid and maybe more economical compared to other methods to perform. Further studies are required in order to investigate the impact of the SNP of CYP2B6 gene on the clinical response to drugs that are substrates for this gene. 2006 Thesis NonPeerReviewed text en https://ir.uitm.edu.my/id/eprint/99045/1/99045.PDF Design and development of a PCR method for detection of SNP of CYP2B6 / Tengku Aliaa Dayana Tuan Mohd Hashim. (2006) Degree thesis, thesis, Universiti Teknologi MARA (Kampus Puncak Alam).
institution Universiti Teknologi Mara
building Tun Abdul Razak Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Teknologi Mara
content_source UiTM Institutional Repository
url_provider http://ir.uitm.edu.my/
language English
topic RM Therapeutics. Pharmacology
RS Pharmacy and materia medica
spellingShingle RM Therapeutics. Pharmacology
RS Pharmacy and materia medica
Tuan Mohd Hashim, Tengku Aliaa Dayana
Design and development of a PCR method for detection of SNP of CYP2B6 / Tengku Aliaa Dayana Tuan Mohd Hashim
description Cytochrome P450 286 is an enzyme that involved in the biotransformation of many clinically important drugs. Genetic polymorphisms of the CYP2B6 gene are an important factor that contributes to the inter-individual and inter-ethnic variability in the systemic exposure, therapeutic and toxic responses to CYP2B6 substrate drugs. Therefore, it is important to develop a method to identify and determine all of the genetic variants in a CYP2B6 gene that responsible for altered CYP2B6 expression. The aim of this study was to design and develop rapid and simple method for the detection of SNP in the CYP2B6 gene. The multiplex, allele-specific PCR method was used. This method involves two-stage procedures, which were first and second PCR. First PCR was used to amplify 3 exons, which were exon 4, exon 5+6, and exon 9. After that, the products of the first PCR were used as a DNA template and genotyped by allele-specific PCR. The assay detected the following published single nucleotide polymorphisms such as G516T (Gln172His), A785G (Lys262Arg) and C1459T (Arg487Cys). DNA sequencing analysis was performed to further confirmed genotype results obtained from current method. Multiplex PCR with allele-specific genotyping method is simple, reliable, rapid and maybe more economical compared to other methods to perform. Further studies are required in order to investigate the impact of the SNP of CYP2B6 gene on the clinical response to drugs that are substrates for this gene.
format Thesis
author Tuan Mohd Hashim, Tengku Aliaa Dayana
author_facet Tuan Mohd Hashim, Tengku Aliaa Dayana
author_sort Tuan Mohd Hashim, Tengku Aliaa Dayana
title Design and development of a PCR method for detection of SNP of CYP2B6 / Tengku Aliaa Dayana Tuan Mohd Hashim
title_short Design and development of a PCR method for detection of SNP of CYP2B6 / Tengku Aliaa Dayana Tuan Mohd Hashim
title_full Design and development of a PCR method for detection of SNP of CYP2B6 / Tengku Aliaa Dayana Tuan Mohd Hashim
title_fullStr Design and development of a PCR method for detection of SNP of CYP2B6 / Tengku Aliaa Dayana Tuan Mohd Hashim
title_full_unstemmed Design and development of a PCR method for detection of SNP of CYP2B6 / Tengku Aliaa Dayana Tuan Mohd Hashim
title_sort design and development of a pcr method for detection of snp of cyp2b6 / tengku aliaa dayana tuan mohd hashim
publishDate 2006
url https://ir.uitm.edu.my/id/eprint/99045/1/99045.PDF
https://ir.uitm.edu.my/id/eprint/99045/
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score 13.211869

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