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Determination of oxidative DNA damage and its correlation with malondialdehyde (MDA) level / Siti Nor Fuadah Sapuan

Oxidative DNA damage was investigated after the intraperitoneal administration of paraquat (PQ) 70 mg/kg in mice by using the comet assay in combination with a specific endonuclease, formamidopyrimidine DNA glycosylase (FPG). On the other hand, the product of lipid peroxidation, malondiladehyde (MDA...

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Main Author: Sapuan, Siti Nor Fuadah
Format: Thesis
Language:English
Published: 2006
Subjects:
QD Chemistry
DNA. Deoxyribonucleic acids
RM Therapeutics. Pharmacology
RS Pharmacy and materia medica
Online Access:https://ir.uitm.edu.my/id/eprint/98927/1/98927.PDF
https://ir.uitm.edu.my/id/eprint/98927/
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spelling my.uitm.ir.989272024-07-28T15:57:03Z https://ir.uitm.edu.my/id/eprint/98927/ Determination of oxidative DNA damage and its correlation with malondialdehyde (MDA) level / Siti Nor Fuadah Sapuan Sapuan, Siti Nor Fuadah QD Chemistry DNA. Deoxyribonucleic acids RM Therapeutics. Pharmacology RS Pharmacy and materia medica Oxidative DNA damage was investigated after the intraperitoneal administration of paraquat (PQ) 70 mg/kg in mice by using the comet assay in combination with a specific endonuclease, formamidopyrimidine DNA glycosylase (FPG). On the other hand, the product of lipid peroxidation, malondiladehyde (MDA) was determined by using HPLC method as a measure of oxidative stress. The correlation between oxidative DNA damage and lipid peroxidation was also investigated. The mice were divided into two groups, a PQ-treated group (n = 6) and a control group (n = 6). The former group received PQ (70 mg/kg, i.p.) while the latter, normal saline (i.p.). After 24 hours, the blood and lungs of the mice were collected for the measurement of oxidative DNA damage by the comet assay while MDA was measured by HPLC in lung and plasma samples. FPG was used in combination with the comet assay to specifically detect oxidative DNA damage. For MDA determination, lung and plasma samples were derivatised with 2,4- dinitrophenylhydrazine (DNPH) before HPLC analysis. The results showed no pronounced oxidative DNA damage in the PQ-treated group both in the presence and absence of FPG. There was no significant DNA damage in the PQ-treated group compared to controls. MDA lung levels were not different from those of the controls but MDA plasma levels were higher than those of controls. It is not certain whether lipid peroxidation had occurred in the lungs since MDA lung levels were not different from those of controls. Since DNA damage did not occur with PQ administration, no correlation could be made between the increase in plasma MDA levels (which is an indication of lipid peroxidation) and oxidative DNA damage. Further studies need to be carried out in order to correlate oxidative DNA damage and oxidative stress as measured by an enhanced MDA level. 2006 Thesis NonPeerReviewed text en https://ir.uitm.edu.my/id/eprint/98927/1/98927.PDF Determination of oxidative DNA damage and its correlation with malondialdehyde (MDA) level / Siti Nor Fuadah Sapuan. (2006) Degree thesis, thesis, Universiti Teknologi MARA (Kampus Puncak Alam).
institution Universiti Teknologi Mara
building Tun Abdul Razak Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Teknologi Mara
content_source UiTM Institutional Repository
url_provider http://ir.uitm.edu.my/
language English
topic QD Chemistry
DNA. Deoxyribonucleic acids
RM Therapeutics. Pharmacology
RS Pharmacy and materia medica
spellingShingle QD Chemistry
DNA. Deoxyribonucleic acids
RM Therapeutics. Pharmacology
RS Pharmacy and materia medica
Sapuan, Siti Nor Fuadah
Determination of oxidative DNA damage and its correlation with malondialdehyde (MDA) level / Siti Nor Fuadah Sapuan
description Oxidative DNA damage was investigated after the intraperitoneal administration of paraquat (PQ) 70 mg/kg in mice by using the comet assay in combination with a specific endonuclease, formamidopyrimidine DNA glycosylase (FPG). On the other hand, the product of lipid peroxidation, malondiladehyde (MDA) was determined by using HPLC method as a measure of oxidative stress. The correlation between oxidative DNA damage and lipid peroxidation was also investigated. The mice were divided into two groups, a PQ-treated group (n = 6) and a control group (n = 6). The former group received PQ (70 mg/kg, i.p.) while the latter, normal saline (i.p.). After 24 hours, the blood and lungs of the mice were collected for the measurement of oxidative DNA damage by the comet assay while MDA was measured by HPLC in lung and plasma samples. FPG was used in combination with the comet assay to specifically detect oxidative DNA damage. For MDA determination, lung and plasma samples were derivatised with 2,4- dinitrophenylhydrazine (DNPH) before HPLC analysis. The results showed no pronounced oxidative DNA damage in the PQ-treated group both in the presence and absence of FPG. There was no significant DNA damage in the PQ-treated group compared to controls. MDA lung levels were not different from those of the controls but MDA plasma levels were higher than those of controls. It is not certain whether lipid peroxidation had occurred in the lungs since MDA lung levels were not different from those of controls. Since DNA damage did not occur with PQ administration, no correlation could be made between the increase in plasma MDA levels (which is an indication of lipid peroxidation) and oxidative DNA damage. Further studies need to be carried out in order to correlate oxidative DNA damage and oxidative stress as measured by an enhanced MDA level.
format Thesis
author Sapuan, Siti Nor Fuadah
author_facet Sapuan, Siti Nor Fuadah
author_sort Sapuan, Siti Nor Fuadah
title Determination of oxidative DNA damage and its correlation with malondialdehyde (MDA) level / Siti Nor Fuadah Sapuan
title_short Determination of oxidative DNA damage and its correlation with malondialdehyde (MDA) level / Siti Nor Fuadah Sapuan
title_full Determination of oxidative DNA damage and its correlation with malondialdehyde (MDA) level / Siti Nor Fuadah Sapuan
title_fullStr Determination of oxidative DNA damage and its correlation with malondialdehyde (MDA) level / Siti Nor Fuadah Sapuan
title_full_unstemmed Determination of oxidative DNA damage and its correlation with malondialdehyde (MDA) level / Siti Nor Fuadah Sapuan
title_sort determination of oxidative dna damage and its correlation with malondialdehyde (mda) level / siti nor fuadah sapuan
publishDate 2006
url https://ir.uitm.edu.my/id/eprint/98927/1/98927.PDF
https://ir.uitm.edu.my/id/eprint/98927/
_version_ 1806422128059219968
score 13.211869

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