Cover Image

Genetic identification of amphibalanus amphitrite (darwin, 1854) from Bagan Pasir and Pasir Penambang, Kuala Selangor / Nurul Nasuha Roseman

Barnacle is a marine sessile crustacean that inhabits the intertidal areas of tropical and temperate waters worldwide. Barnacles in family Balanidae belongs to superorder Thoracica and they consist of acorn barnacles and stalkless barnacles. In Malaysia, only a few studies have been done on barnacle...

Full description

Saved in:
Bibliographic Details
Main Author: Roseman, Nurul Nasuha
Format: Thesis
Language:English
Published: 2019
Online Access:https://ir.uitm.edu.my/id/eprint/89116/1/89116.pdf
https://ir.uitm.edu.my/id/eprint/89116/
Tags: Add Tag
No Tags, Be the first to tag this record!
id my.uitm.ir.89116
record_format eprints
spelling my.uitm.ir.891162024-08-16T03:00:26Z https://ir.uitm.edu.my/id/eprint/89116/ Genetic identification of amphibalanus amphitrite (darwin, 1854) from Bagan Pasir and Pasir Penambang, Kuala Selangor / Nurul Nasuha Roseman Roseman, Nurul Nasuha Barnacle is a marine sessile crustacean that inhabits the intertidal areas of tropical and temperate waters worldwide. Barnacles in family Balanidae belongs to superorder Thoracica and they consist of acorn barnacles and stalkless barnacles. In Malaysia, only a few studies have been done on barnacles in family Balanidae. In this present study, the molecular identification (16S rDNA) of barnacles in family Balanidae from Bagan Pasir and Pasir Penambang were investigated. Molecular identification is more accurate and faster compared to morphological classification as the morphology of the barnacles might differ due to geographical isolation. The objectives of this study were to assess the genetic information of barnacles in family Balanidae and to identify the specific species of barnacles in family Balanidae based on 16S rDNA identification. Four samples from Bagan Pasir and three samples from Pasir Penambang were studied to identify the specific species of the barnacles. The mitochondrial 16S molecular marker was used to identify the species and 500 bp band was observed on 1.5% agarose gel electrophoresis gel. The results showed that only Amphibalanus amphitrite (Darwin, 1854) was identified from both sampling sites. The phylogenetic tree constructed also stated that all the samples were identified as A.amphitrite (Darwin, 1854) and they were clustered in one clade. However, one sample from Bagan Pasir showed some difference in their DNA sequence as it was located at a different node from the other samples under the same cluster. In future, the molecular identification of the barnacles could have been done by using other molecular marker such as 12S rDNA, COI and the nuclear molecular marker 18S rDNA. The sampling site should also include other places in Selangor coast as to obtain different species of barnacles. 2019 Thesis NonPeerReviewed text en https://ir.uitm.edu.my/id/eprint/89116/1/89116.pdf Genetic identification of amphibalanus amphitrite (darwin, 1854) from Bagan Pasir and Pasir Penambang, Kuala Selangor / Nurul Nasuha Roseman. (2019) Masters thesis, thesis, Universiti Teknologi MARA (UiTM). <http://terminalib.uitm.edu.my/89116.pdf>
institution Universiti Teknologi Mara
building Tun Abdul Razak Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Teknologi Mara
content_source UiTM Institutional Repository
url_provider http://ir.uitm.edu.my/
language English
description Barnacle is a marine sessile crustacean that inhabits the intertidal areas of tropical and temperate waters worldwide. Barnacles in family Balanidae belongs to superorder Thoracica and they consist of acorn barnacles and stalkless barnacles. In Malaysia, only a few studies have been done on barnacles in family Balanidae. In this present study, the molecular identification (16S rDNA) of barnacles in family Balanidae from Bagan Pasir and Pasir Penambang were investigated. Molecular identification is more accurate and faster compared to morphological classification as the morphology of the barnacles might differ due to geographical isolation. The objectives of this study were to assess the genetic information of barnacles in family Balanidae and to identify the specific species of barnacles in family Balanidae based on 16S rDNA identification. Four samples from Bagan Pasir and three samples from Pasir Penambang were studied to identify the specific species of the barnacles. The mitochondrial 16S molecular marker was used to identify the species and 500 bp band was observed on 1.5% agarose gel electrophoresis gel. The results showed that only Amphibalanus amphitrite (Darwin, 1854) was identified from both sampling sites. The phylogenetic tree constructed also stated that all the samples were identified as A.amphitrite (Darwin, 1854) and they were clustered in one clade. However, one sample from Bagan Pasir showed some difference in their DNA sequence as it was located at a different node from the other samples under the same cluster. In future, the molecular identification of the barnacles could have been done by using other molecular marker such as 12S rDNA, COI and the nuclear molecular marker 18S rDNA. The sampling site should also include other places in Selangor coast as to obtain different species of barnacles.
format Thesis
author Roseman, Nurul Nasuha
spellingShingle Roseman, Nurul Nasuha
Genetic identification of amphibalanus amphitrite (darwin, 1854) from Bagan Pasir and Pasir Penambang, Kuala Selangor / Nurul Nasuha Roseman
author_facet Roseman, Nurul Nasuha
author_sort Roseman, Nurul Nasuha
title Genetic identification of amphibalanus amphitrite (darwin, 1854) from Bagan Pasir and Pasir Penambang, Kuala Selangor / Nurul Nasuha Roseman
title_short Genetic identification of amphibalanus amphitrite (darwin, 1854) from Bagan Pasir and Pasir Penambang, Kuala Selangor / Nurul Nasuha Roseman
title_full Genetic identification of amphibalanus amphitrite (darwin, 1854) from Bagan Pasir and Pasir Penambang, Kuala Selangor / Nurul Nasuha Roseman
title_fullStr Genetic identification of amphibalanus amphitrite (darwin, 1854) from Bagan Pasir and Pasir Penambang, Kuala Selangor / Nurul Nasuha Roseman
title_full_unstemmed Genetic identification of amphibalanus amphitrite (darwin, 1854) from Bagan Pasir and Pasir Penambang, Kuala Selangor / Nurul Nasuha Roseman
title_sort genetic identification of amphibalanus amphitrite (darwin, 1854) from bagan pasir and pasir penambang, kuala selangor / nurul nasuha roseman
publishDate 2019
url https://ir.uitm.edu.my/id/eprint/89116/1/89116.pdf
https://ir.uitm.edu.my/id/eprint/89116/
_version_ 1808975932796436480
score 13.211869

Similar Items