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Biodegradation of pharmaceutical compounds caffeine and carbamazepine using gram-positive bacteria / Najihah Mohamad Nasir

This study aims to evaluate the potential of selected bacteria in caffeine and carbamazepine degradation. The objectives of this study are to screen potential bacteria in degradation of caffeine and carbamazepine, to optimise degradation parameters of caffeine and carbamazepine as well as to evaluat...

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Main Author: Mohamad Nasir, Najihah
Format: Thesis
Language:English
Published: 2019
Subjects:
TD Environmental technology. Sanitary engineering
Online Access:https://ir.uitm.edu.my/id/eprint/84335/1/84335.pdf
https://ir.uitm.edu.my/id/eprint/84335/
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spelling my.uitm.ir.843352024-10-24T08:47:14Z https://ir.uitm.edu.my/id/eprint/84335/ Biodegradation of pharmaceutical compounds caffeine and carbamazepine using gram-positive bacteria / Najihah Mohamad Nasir Mohamad Nasir, Najihah TD Environmental technology. Sanitary engineering This study aims to evaluate the potential of selected bacteria in caffeine and carbamazepine degradation. The objectives of this study are to screen potential bacteria in degradation of caffeine and carbamazepine, to optimise degradation parameters of caffeine and carbamazepine as well as to evaluate degrading enzymes for prediction of degradation pathways. Methodologies included screening of bacteria resistance towards caffeine and carbamazepine and optimisation parameters of initial concentrations, initial pH and temperature for degradation of caffeine and carbamazepine. Then, mixed culture study was conducted at the selected optimum condition. At the same time, bacteria growth for Rhodococcus zopfii and Leifsonia shinshuensis was determined to support the degradation result. In addition, enzyme assays for oxygen oxidoreductase, aldehyde oxidase, catechol 1,2 dioxygenase and catechol 2,3 dioxygenase were also conducted. The result demonstrated that bacteria R. zopfii and L. shinshuensis were selected due to growth and potential in utilising caffeine and carbamazepine as nutrient source for degradation. For single culture degradation study, the 10 mgL-1, pH 7 and 40 °C were selected as optimum condition for degradation of caffeine and carbamazepine. Optimum normalised degradation percentage of caffeine by R. zopfii was 72.53 ± 0.10 and L. shinshuensis was only 38.49 ± 0.01. For carbamazepine, both R. zopfii and L.shinshuensis achieved high normalised degradation percentage of 99.99 ± 0.00. Degradation of caffeine was not correlated to bacteria growth meanwhile, degradation of carbamazepine was linearly correlated to the bacteria growth. The result also revealed antagonistic interaction between R. zopfii and L. shinshuensis in mixed culture. Furthermore, enzyme assay for bacteria in caffeine showed presence of enzymes oxygen oxidoreductase and aldehyde oxidase, meanwhile, oxygen oxidoreductase, aldehyde oxidase, catechol 1,2 dioxygenase and catechol 2,3 dioxygenase were determined during carbamazepine degradation. 2019 Thesis NonPeerReviewed text en https://ir.uitm.edu.my/id/eprint/84335/1/84335.pdf Biodegradation of pharmaceutical compounds caffeine and carbamazepine using gram-positive bacteria / Najihah Mohamad Nasir. (2019) Masters thesis, thesis, Universiti Teknologi MARA (UiTM).
institution Universiti Teknologi Mara
building Tun Abdul Razak Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Teknologi Mara
content_source UiTM Institutional Repository
url_provider http://ir.uitm.edu.my/
language English
topic TD Environmental technology. Sanitary engineering
spellingShingle TD Environmental technology. Sanitary engineering
Mohamad Nasir, Najihah
Biodegradation of pharmaceutical compounds caffeine and carbamazepine using gram-positive bacteria / Najihah Mohamad Nasir
description This study aims to evaluate the potential of selected bacteria in caffeine and carbamazepine degradation. The objectives of this study are to screen potential bacteria in degradation of caffeine and carbamazepine, to optimise degradation parameters of caffeine and carbamazepine as well as to evaluate degrading enzymes for prediction of degradation pathways. Methodologies included screening of bacteria resistance towards caffeine and carbamazepine and optimisation parameters of initial concentrations, initial pH and temperature for degradation of caffeine and carbamazepine. Then, mixed culture study was conducted at the selected optimum condition. At the same time, bacteria growth for Rhodococcus zopfii and Leifsonia shinshuensis was determined to support the degradation result. In addition, enzyme assays for oxygen oxidoreductase, aldehyde oxidase, catechol 1,2 dioxygenase and catechol 2,3 dioxygenase were also conducted. The result demonstrated that bacteria R. zopfii and L. shinshuensis were selected due to growth and potential in utilising caffeine and carbamazepine as nutrient source for degradation. For single culture degradation study, the 10 mgL-1, pH 7 and 40 °C were selected as optimum condition for degradation of caffeine and carbamazepine. Optimum normalised degradation percentage of caffeine by R. zopfii was 72.53 ± 0.10 and L. shinshuensis was only 38.49 ± 0.01. For carbamazepine, both R. zopfii and L.shinshuensis achieved high normalised degradation percentage of 99.99 ± 0.00. Degradation of caffeine was not correlated to bacteria growth meanwhile, degradation of carbamazepine was linearly correlated to the bacteria growth. The result also revealed antagonistic interaction between R. zopfii and L. shinshuensis in mixed culture. Furthermore, enzyme assay for bacteria in caffeine showed presence of enzymes oxygen oxidoreductase and aldehyde oxidase, meanwhile, oxygen oxidoreductase, aldehyde oxidase, catechol 1,2 dioxygenase and catechol 2,3 dioxygenase were determined during carbamazepine degradation.
format Thesis
author Mohamad Nasir, Najihah
author_facet Mohamad Nasir, Najihah
author_sort Mohamad Nasir, Najihah
title Biodegradation of pharmaceutical compounds caffeine and carbamazepine using gram-positive bacteria / Najihah Mohamad Nasir
title_short Biodegradation of pharmaceutical compounds caffeine and carbamazepine using gram-positive bacteria / Najihah Mohamad Nasir
title_full Biodegradation of pharmaceutical compounds caffeine and carbamazepine using gram-positive bacteria / Najihah Mohamad Nasir
title_fullStr Biodegradation of pharmaceutical compounds caffeine and carbamazepine using gram-positive bacteria / Najihah Mohamad Nasir
title_full_unstemmed Biodegradation of pharmaceutical compounds caffeine and carbamazepine using gram-positive bacteria / Najihah Mohamad Nasir
title_sort biodegradation of pharmaceutical compounds caffeine and carbamazepine using gram-positive bacteria / najihah mohamad nasir
publishDate 2019
url https://ir.uitm.edu.my/id/eprint/84335/1/84335.pdf
https://ir.uitm.edu.my/id/eprint/84335/
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score 13.211869

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