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In vitro root induction of Catharanthus roseus / Siti Nazliah Mohamed Nazir

Catharanthus roseus has high significant study due to its medicinal properties, but there is still lacks of information about the best combination of the hormones of the root induction. Thus, C. roseus are propagated by using plantlets through the techniques of tissue culture. The objectives of the...

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Main Author: Mohamed Nazir, Siti Nazliah
Format: Thesis
Language:English
Published: 2016
Subjects:
Medicinal plants (Culture only)
Online Access:https://ir.uitm.edu.my/id/eprint/83852/1/83852.PDF
https://ir.uitm.edu.my/id/eprint/83852/
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spelling my.uitm.ir.838522024-01-22T02:24:42Z https://ir.uitm.edu.my/id/eprint/83852/ In vitro root induction of Catharanthus roseus / Siti Nazliah Mohamed Nazir Mohamed Nazir, Siti Nazliah Medicinal plants (Culture only) Catharanthus roseus has high significant study due to its medicinal properties, but there is still lacks of information about the best combination of the hormones of the root induction. Thus, C. roseus are propagated by using plantlets through the techniques of tissue culture. The objectives of the study are to investigate the cells formation by subculturing of C. roseus in MS media and to evaluate the performance of C. roseus plantlets by using different concentration of the hormones. The subject was treated with control (C) and four different treatments that consist of different combination of hormones of auxin and cytokinin (T1, T2, T3 and T4). Begins with the preparation of the media, Murashige and Skoog (MS) media was used. The plantlets then were transferred into the media to be incubate at 25°C for 8 weeks with the photoperiod of 16:8 hours. In week 4, the white callus shows friable structure compared to the green callus that is more compact and granular in all treatments. The cross section of the stem exhibit a ring structure of discrete vascular bundle with a narrow cortex situated externally indicates a dicots plants. The leaves of the control environment is generally yellow in color while greenish color observed in the T1 to T4 treatments, especially in T4. In week 8, the roots induction proliferates more in the T4. While the concentration of cytokinin was held constant at 0.5 mg/ml, Tl with equal concentration of auxin-cytokinin mostly show the growth of shoots. T2, T3 and T4 with concentration auxin of 1.0 mg/ml, 1.5 mg/ml, 2.0 mg/ml respectively show the growth of roots. However, the most number of generated roots come from the T4. 2016 Thesis NonPeerReviewed text en https://ir.uitm.edu.my/id/eprint/83852/1/83852.PDF In vitro root induction of Catharanthus roseus / Siti Nazliah Mohamed Nazir. (2016) Degree thesis, thesis, Universiti Teknologi MARA Pahang. <http://terminalib.uitm.edu.my/83852.pdf>
institution Universiti Teknologi Mara
building Tun Abdul Razak Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Teknologi Mara
content_source UiTM Institutional Repository
url_provider http://ir.uitm.edu.my/
language English
topic Medicinal plants (Culture only)
spellingShingle Medicinal plants (Culture only)
Mohamed Nazir, Siti Nazliah
In vitro root induction of Catharanthus roseus / Siti Nazliah Mohamed Nazir
description Catharanthus roseus has high significant study due to its medicinal properties, but there is still lacks of information about the best combination of the hormones of the root induction. Thus, C. roseus are propagated by using plantlets through the techniques of tissue culture. The objectives of the study are to investigate the cells formation by subculturing of C. roseus in MS media and to evaluate the performance of C. roseus plantlets by using different concentration of the hormones. The subject was treated with control (C) and four different treatments that consist of different combination of hormones of auxin and cytokinin (T1, T2, T3 and T4). Begins with the preparation of the media, Murashige and Skoog (MS) media was used. The plantlets then were transferred into the media to be incubate at 25°C for 8 weeks with the photoperiod of 16:8 hours. In week 4, the white callus shows friable structure compared to the green callus that is more compact and granular in all treatments. The cross section of the stem exhibit a ring structure of discrete vascular bundle with a narrow cortex situated externally indicates a dicots plants. The leaves of the control environment is generally yellow in color while greenish color observed in the T1 to T4 treatments, especially in T4. In week 8, the roots induction proliferates more in the T4. While the concentration of cytokinin was held constant at 0.5 mg/ml, Tl with equal concentration of auxin-cytokinin mostly show the growth of shoots. T2, T3 and T4 with concentration auxin of 1.0 mg/ml, 1.5 mg/ml, 2.0 mg/ml respectively show the growth of roots. However, the most number of generated roots come from the T4.
format Thesis
author Mohamed Nazir, Siti Nazliah
author_facet Mohamed Nazir, Siti Nazliah
author_sort Mohamed Nazir, Siti Nazliah
title In vitro root induction of Catharanthus roseus / Siti Nazliah Mohamed Nazir
title_short In vitro root induction of Catharanthus roseus / Siti Nazliah Mohamed Nazir
title_full In vitro root induction of Catharanthus roseus / Siti Nazliah Mohamed Nazir
title_fullStr In vitro root induction of Catharanthus roseus / Siti Nazliah Mohamed Nazir
title_full_unstemmed In vitro root induction of Catharanthus roseus / Siti Nazliah Mohamed Nazir
title_sort in vitro root induction of catharanthus roseus / siti nazliah mohamed nazir
publishDate 2016
url https://ir.uitm.edu.my/id/eprint/83852/1/83852.PDF
https://ir.uitm.edu.my/id/eprint/83852/
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score 13.211869

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