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Sterilization protocol of seed culture for both of Solanum lycopersicum and Capsicum annuum / Nurul Nasuha Shamsuddin

Tomato (Solanum lycopersicum) is a freshly vegetable used all over the world. Solanum lycopersicum is belongs to the family of Solanaceae and chilli is a fruits or berries of plants belonging to the genus Capsicum. In this research study, sterilization can be defined as the removal of all microorgan...

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Main Author: Shamsuddin, Nurul Nasuha
Format: Thesis
Language:English
Published: 2016
Subjects:
Bacteria
Online Access:https://ir.uitm.edu.my/id/eprint/83846/1/83846.PDF
https://ir.uitm.edu.my/id/eprint/83846/
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spelling my.uitm.ir.838462024-01-22T03:06:40Z https://ir.uitm.edu.my/id/eprint/83846/ Sterilization protocol of seed culture for both of Solanum lycopersicum and Capsicum annuum / Nurul Nasuha Shamsuddin Shamsuddin, Nurul Nasuha Bacteria Tomato (Solanum lycopersicum) is a freshly vegetable used all over the world. Solanum lycopersicum is belongs to the family of Solanaceae and chilli is a fruits or berries of plants belonging to the genus Capsicum. In this research study, sterilization can be defined as the removal of all microorganisms and other pathogens from an object or surface by treating it with chemicals or subjecting it to high heat or radiation. The different sterilization chemical were used which were commercial soap, Clorox, ethanol, Tween-20 and sanitizer. The protocol which approaching zero contamination were the protocol 1 using commercial soap, Clorox and ethanol. In this protocol, the chemical were contributing zero contamination it the present of commercial soap. The present of commercial soap as sterilization chemical because the chemical substance of chloroxylenol and aromatic compound (derived from the phenol. This chemical substance that was killed the bacteria and fungus present in seed culture. The objectives of this research study were to determine the optimum sterilization protocol for both seed culture by using five different sterilization protocols and to investigate the effect of sterilization protocol in both seed culture germination in one month duration. In this research, the protocol 1 has 6 step of sterilization, protocol 2 have 4 steps of sterilization, protocol 3 and 4 have 2 step sterilization and protocol 5 have 6 step of sterilization. In this research study, the technique was applied in sterilization protocol such as soaking, dipping and washing the seed culture. This finding showed, the protocol 1 that was possibility for zero contamination determine through the lack number of contamination in culture plant. Besides, protocol 3 has showed higher number of contamination because the seed only washed with 70% ethanol mixed with a few drops of Tween-20. The uses of commercial soap in sterilization protocol 1 also showed successful effect to the seed germination. 2016 Thesis NonPeerReviewed text en https://ir.uitm.edu.my/id/eprint/83846/1/83846.PDF Sterilization protocol of seed culture for both of Solanum lycopersicum and Capsicum annuum / Nurul Nasuha Shamsuddin. (2016) Degree thesis, thesis, Universiti Teknologi MARA Pahang. <http://terminalib.uitm.edu.my/83846.pdf>
institution Universiti Teknologi Mara
building Tun Abdul Razak Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Teknologi Mara
content_source UiTM Institutional Repository
url_provider http://ir.uitm.edu.my/
language English
topic Bacteria
spellingShingle Bacteria
Shamsuddin, Nurul Nasuha
Sterilization protocol of seed culture for both of Solanum lycopersicum and Capsicum annuum / Nurul Nasuha Shamsuddin
description Tomato (Solanum lycopersicum) is a freshly vegetable used all over the world. Solanum lycopersicum is belongs to the family of Solanaceae and chilli is a fruits or berries of plants belonging to the genus Capsicum. In this research study, sterilization can be defined as the removal of all microorganisms and other pathogens from an object or surface by treating it with chemicals or subjecting it to high heat or radiation. The different sterilization chemical were used which were commercial soap, Clorox, ethanol, Tween-20 and sanitizer. The protocol which approaching zero contamination were the protocol 1 using commercial soap, Clorox and ethanol. In this protocol, the chemical were contributing zero contamination it the present of commercial soap. The present of commercial soap as sterilization chemical because the chemical substance of chloroxylenol and aromatic compound (derived from the phenol. This chemical substance that was killed the bacteria and fungus present in seed culture. The objectives of this research study were to determine the optimum sterilization protocol for both seed culture by using five different sterilization protocols and to investigate the effect of sterilization protocol in both seed culture germination in one month duration. In this research, the protocol 1 has 6 step of sterilization, protocol 2 have 4 steps of sterilization, protocol 3 and 4 have 2 step sterilization and protocol 5 have 6 step of sterilization. In this research study, the technique was applied in sterilization protocol such as soaking, dipping and washing the seed culture. This finding showed, the protocol 1 that was possibility for zero contamination determine through the lack number of contamination in culture plant. Besides, protocol 3 has showed higher number of contamination because the seed only washed with 70% ethanol mixed with a few drops of Tween-20. The uses of commercial soap in sterilization protocol 1 also showed successful effect to the seed germination.
format Thesis
author Shamsuddin, Nurul Nasuha
author_facet Shamsuddin, Nurul Nasuha
author_sort Shamsuddin, Nurul Nasuha
title Sterilization protocol of seed culture for both of Solanum lycopersicum and Capsicum annuum / Nurul Nasuha Shamsuddin
title_short Sterilization protocol of seed culture for both of Solanum lycopersicum and Capsicum annuum / Nurul Nasuha Shamsuddin
title_full Sterilization protocol of seed culture for both of Solanum lycopersicum and Capsicum annuum / Nurul Nasuha Shamsuddin
title_fullStr Sterilization protocol of seed culture for both of Solanum lycopersicum and Capsicum annuum / Nurul Nasuha Shamsuddin
title_full_unstemmed Sterilization protocol of seed culture for both of Solanum lycopersicum and Capsicum annuum / Nurul Nasuha Shamsuddin
title_sort sterilization protocol of seed culture for both of solanum lycopersicum and capsicum annuum / nurul nasuha shamsuddin
publishDate 2016
url https://ir.uitm.edu.my/id/eprint/83846/1/83846.PDF
https://ir.uitm.edu.my/id/eprint/83846/
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score 13.211869

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