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Extraction and characterization of lipase from seed of Hevea brasiliensis / Nortasa Shakri

Acetone powder was obtained from seed of Hevea brasiliensis as the source crude lipase. Activity of Lipase from ungerminated seed and germinated seed was examined by their ability on hydrolysis of FFA and also selectivity on FFA. Lipase from germinated seed show highest activity on hydrolysis of lon...

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Main Author: Shakri, Nortasa
Format: Student Project
Language:English
Published: 2016
Subjects:
Extraction (Chemistry)
Seeds. Seed technology
Online Access:https://ir.uitm.edu.my/id/eprint/83587/1/83587.PDF
https://ir.uitm.edu.my/id/eprint/83587/
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spelling my.uitm.ir.835872024-01-26T01:32:06Z https://ir.uitm.edu.my/id/eprint/83587/ Extraction and characterization of lipase from seed of Hevea brasiliensis / Nortasa Shakri Shakri, Nortasa Extraction (Chemistry) Seeds. Seed technology Acetone powder was obtained from seed of Hevea brasiliensis as the source crude lipase. Activity of Lipase from ungerminated seed and germinated seed was examined by their ability on hydrolysis of FFA and also selectivity on FFA. Lipase from germinated seed show highest activity on hydrolysis of long chain fatty acid (palm oil) and short chain fatty acid (coconut oil) compared to the ungerminated seed. Lipase activity was more pronounce in coconut oil with short chain fatty acid rather than palm oil with long chain fatty acid. The amount of enzyme activity for germinated seed lipase in hydrolysis of coconut oil is 115 x 10-5 mol.g/h, while for palm oil is 8.17x 10-5 mol.g/h. Lipase from ungerminated seed have low activity on hydrolysis of oil, for coconut oil enzyme activity is 9.96x 10-5mol.g1h and for palm oil is 92.3 x 10-5 mol.g/h. Lipase from seed of Hevea brasiliensis is non-selective toward fatty acid caprylic acid (C:8), capric acid (C:10), lauric acid (C:12), myristic acid (C:14), palmitic acid (C: l6),stearic acid (C: 18). 2016 Student Project NonPeerReviewed text en https://ir.uitm.edu.my/id/eprint/83587/1/83587.PDF Extraction and characterization of lipase from seed of Hevea brasiliensis / Nortasa Shakri. (2016) [Student Project] <http://terminalib.uitm.edu.my/83587.pdf> (Unpublished)
institution Universiti Teknologi Mara
building Tun Abdul Razak Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Teknologi Mara
content_source UiTM Institutional Repository
url_provider http://ir.uitm.edu.my/
language English
topic Extraction (Chemistry)
Seeds. Seed technology
spellingShingle Extraction (Chemistry)
Seeds. Seed technology
Shakri, Nortasa
Extraction and characterization of lipase from seed of Hevea brasiliensis / Nortasa Shakri
description Acetone powder was obtained from seed of Hevea brasiliensis as the source crude lipase. Activity of Lipase from ungerminated seed and germinated seed was examined by their ability on hydrolysis of FFA and also selectivity on FFA. Lipase from germinated seed show highest activity on hydrolysis of long chain fatty acid (palm oil) and short chain fatty acid (coconut oil) compared to the ungerminated seed. Lipase activity was more pronounce in coconut oil with short chain fatty acid rather than palm oil with long chain fatty acid. The amount of enzyme activity for germinated seed lipase in hydrolysis of coconut oil is 115 x 10-5 mol.g/h, while for palm oil is 8.17x 10-5 mol.g/h. Lipase from ungerminated seed have low activity on hydrolysis of oil, for coconut oil enzyme activity is 9.96x 10-5mol.g1h and for palm oil is 92.3 x 10-5 mol.g/h. Lipase from seed of Hevea brasiliensis is non-selective toward fatty acid caprylic acid (C:8), capric acid (C:10), lauric acid (C:12), myristic acid (C:14), palmitic acid (C: l6),stearic acid (C: 18).
format Student Project
author Shakri, Nortasa
author_facet Shakri, Nortasa
author_sort Shakri, Nortasa
title Extraction and characterization of lipase from seed of Hevea brasiliensis / Nortasa Shakri
title_short Extraction and characterization of lipase from seed of Hevea brasiliensis / Nortasa Shakri
title_full Extraction and characterization of lipase from seed of Hevea brasiliensis / Nortasa Shakri
title_fullStr Extraction and characterization of lipase from seed of Hevea brasiliensis / Nortasa Shakri
title_full_unstemmed Extraction and characterization of lipase from seed of Hevea brasiliensis / Nortasa Shakri
title_sort extraction and characterization of lipase from seed of hevea brasiliensis / nortasa shakri
publishDate 2016
url https://ir.uitm.edu.my/id/eprint/83587/1/83587.PDF
https://ir.uitm.edu.my/id/eprint/83587/
_version_ 1789429206924394496
score 13.211869

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