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Screening of antagonistic bacteria from healthy pineapple variety MD2 and its potential against bacterial heart rot disease / Naimah Husin

Bacterial heart rot disease caused by pathogenic bacteria Erwinia chrysanthemi is one of the common diseases of pineapple. Current industry practices for controlling the disease through cultural practices and application of chemical synthetic of Benomyl which is not a long lasting control method. Th...

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Main Author: Husin, Naimah
Format: Thesis
Language:English
Published: 2019
Online Access:https://ir.uitm.edu.my/id/eprint/79342/1/79342.pdf
https://ir.uitm.edu.my/id/eprint/79342/
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spelling my.uitm.ir.793422023-09-19T04:56:11Z https://ir.uitm.edu.my/id/eprint/79342/ Screening of antagonistic bacteria from healthy pineapple variety MD2 and its potential against bacterial heart rot disease / Naimah Husin Husin, Naimah Bacterial heart rot disease caused by pathogenic bacteria Erwinia chrysanthemi is one of the common diseases of pineapple. Current industry practices for controlling the disease through cultural practices and application of chemical synthetic of Benomyl which is not a long lasting control method. This study aims to evaluate the potential of beneficial bacteria isolated from healthy pineapple leaves against the disease pathogen. The disease pathogen was isolated from symptomatic pineapple leaves with the appearance of water soaked and heart rot disease symptoms such as rotten basal tissues. Pathogenicity test was conducted to fulfil the Koch's Postulates. The same procedure for isolation of beneficial bacteria from healthy pineapple plant variety MD2 was carried out. A total of 25 bacterial isolates were successfully isolated from the healthy pineapple leaves and tested against the bacterial pathogen via agar well diffusion method. In vitro assay was utilized to measure the ability of isolated beneficial bacteria in the form of inhibition zone and its growth on media. An isolate identified as Bacillus cereus (BC3) showed the potential of antagonistic activity towards the pathogen. The isolate was further assessed in vivo under rain shelter conditions. The result showed that the isolate was able to suppress the disease symptoms. This study suggested that B. cereus (BC3) could be further explored for its potential as biological control agent for controlling the disease under field condition. 2019 Thesis NonPeerReviewed text en https://ir.uitm.edu.my/id/eprint/79342/1/79342.pdf Screening of antagonistic bacteria from healthy pineapple variety MD2 and its potential against bacterial heart rot disease / Naimah Husin. (2019) Masters thesis, thesis, Universiti Teknologi MARA (UiTM).
institution Universiti Teknologi Mara
building Tun Abdul Razak Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Teknologi Mara
content_source UiTM Institutional Repository
url_provider http://ir.uitm.edu.my/
language English
description Bacterial heart rot disease caused by pathogenic bacteria Erwinia chrysanthemi is one of the common diseases of pineapple. Current industry practices for controlling the disease through cultural practices and application of chemical synthetic of Benomyl which is not a long lasting control method. This study aims to evaluate the potential of beneficial bacteria isolated from healthy pineapple leaves against the disease pathogen. The disease pathogen was isolated from symptomatic pineapple leaves with the appearance of water soaked and heart rot disease symptoms such as rotten basal tissues. Pathogenicity test was conducted to fulfil the Koch's Postulates. The same procedure for isolation of beneficial bacteria from healthy pineapple plant variety MD2 was carried out. A total of 25 bacterial isolates were successfully isolated from the healthy pineapple leaves and tested against the bacterial pathogen via agar well diffusion method. In vitro assay was utilized to measure the ability of isolated beneficial bacteria in the form of inhibition zone and its growth on media. An isolate identified as Bacillus cereus (BC3) showed the potential of antagonistic activity towards the pathogen. The isolate was further assessed in vivo under rain shelter conditions. The result showed that the isolate was able to suppress the disease symptoms. This study suggested that B. cereus (BC3) could be further explored for its potential as biological control agent for controlling the disease under field condition.
format Thesis
author Husin, Naimah
spellingShingle Husin, Naimah
Screening of antagonistic bacteria from healthy pineapple variety MD2 and its potential against bacterial heart rot disease / Naimah Husin
author_facet Husin, Naimah
author_sort Husin, Naimah
title Screening of antagonistic bacteria from healthy pineapple variety MD2 and its potential against bacterial heart rot disease / Naimah Husin
title_short Screening of antagonistic bacteria from healthy pineapple variety MD2 and its potential against bacterial heart rot disease / Naimah Husin
title_full Screening of antagonistic bacteria from healthy pineapple variety MD2 and its potential against bacterial heart rot disease / Naimah Husin
title_fullStr Screening of antagonistic bacteria from healthy pineapple variety MD2 and its potential against bacterial heart rot disease / Naimah Husin
title_full_unstemmed Screening of antagonistic bacteria from healthy pineapple variety MD2 and its potential against bacterial heart rot disease / Naimah Husin
title_sort screening of antagonistic bacteria from healthy pineapple variety md2 and its potential against bacterial heart rot disease / naimah husin
publishDate 2019
url https://ir.uitm.edu.my/id/eprint/79342/1/79342.pdf
https://ir.uitm.edu.my/id/eprint/79342/
_version_ 1778165884918956032
score 13.211869

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