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Extraction of Hibiscus rosa sinensis leaves using ultrasonic water bath in determining the phenolic content / Nor Fazlina Abdul Ghani and Dr Nor Fariza Ismail

Hibiscus rosa-sinensis grows at many places around the globe. Hibiscus rosa-sinensis contains numerous classes of auxiliary metabolites, counting flavonoids, anthocyanins, terpenoids, steroids, polysaccharides, alkaloids, amino acids, lipids, and naphthalene group. Different kind of studies show tha...

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Main Authors: Abdul Ghani, Nor Fazlina, Ismail, Nor Fariza
Format: Conference or Workshop Item
Language:English
Published: 2021
Subjects:
QD Chemistry
Extraction (Chemistry)
Online Access:https://ir.uitm.edu.my/id/eprint/78947/1/78947.pdf
https://ir.uitm.edu.my/id/eprint/78947/
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spelling my.uitm.ir.789472023-06-12T04:31:53Z https://ir.uitm.edu.my/id/eprint/78947/ Extraction of Hibiscus rosa sinensis leaves using ultrasonic water bath in determining the phenolic content / Nor Fazlina Abdul Ghani and Dr Nor Fariza Ismail Abdul Ghani, Nor Fazlina Ismail, Nor Fariza QD Chemistry Extraction (Chemistry) Hibiscus rosa-sinensis grows at many places around the globe. Hibiscus rosa-sinensis contains numerous classes of auxiliary metabolites, counting flavonoids, anthocyanins, terpenoids, steroids, polysaccharides, alkaloids, amino acids, lipids, and naphthalene group. Different kind of studies show that parts of Hibiscus rosa-sinensis plants possess antioxidant, antimicrobial, antidiabetic, antiulcer, hepatoprotective, antifertility, antigenotoxic and anti-inflammatory properties that help in treatment of many diseases. Therefore, Hibiscus rosa-sinensis plant has been identified as one of many medicinal plants that possesses various potential in therapeutic applications. Bioactive compound extraction on plants done by several methods like conventional method, Microwave Assisted Extraction (MAE) method etc. However, none of the study has been done on extraction of bioactive compounds extracted from Hibiscus rosa-sinensis leaves using Ultrasonic Assisted Extraction (UAE) method. Hence, there is essential to investigate on the benefits of the Hibiscus rosa-sinensis leaves for the future used by using UAE method for extraction. The objectives of this study are to extract bioactive compound extract from Hibiscus rosa-sinensis leaves by using ultrasonic water bath using different solvents like ethanol, methanol, ethyl acetate and acetone and to analyse phenolic profile from the extract. The solvent is chosen based on the polarity due to the presence of hydroxyl group in determining the phenolic compound. Hibiscus rosa-sinensis leaves is prepared by drying at 50°C and pestled before extraction by different solvent. After that, the sample is placed in ultrasonic water bath at different time (5 min, 15 min, 30 min, 45 min, 60 min) and then the solvent is removed by using rotary evaporator. The result shows the highest extraction yield by UAE method is methanol and then followed by ethanol, acetone, and ethyl acetate. After extraction, UV-VIS spectrophotometer was used to determine the phenolic content in Hibiscus rosa-sinensis leaves. Even though methanol has the highest yield of extraction, it is not the best solvent to determine the phenolic content when comparing it with standard calibration curve of Gallic acid. However, as for ethyl acetate, it has the lowest extraction yield but able to determine the phenolic content. As a conclusion, the yield of extraction does not affect the determination of the phenolic content. 2021 Conference or Workshop Item PeerReviewed text en https://ir.uitm.edu.my/id/eprint/78947/1/78947.pdf Extraction of Hibiscus rosa sinensis leaves using ultrasonic water bath in determining the phenolic content / Nor Fazlina Abdul Ghani and Dr Nor Fariza Ismail. (2021) In: UNSPECIFIED.
institution Universiti Teknologi Mara
building Tun Abdul Razak Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Teknologi Mara
content_source UiTM Institutional Repository
url_provider http://ir.uitm.edu.my/
language English
topic QD Chemistry
Extraction (Chemistry)
spellingShingle QD Chemistry
Extraction (Chemistry)
Abdul Ghani, Nor Fazlina
Ismail, Nor Fariza
Extraction of Hibiscus rosa sinensis leaves using ultrasonic water bath in determining the phenolic content / Nor Fazlina Abdul Ghani and Dr Nor Fariza Ismail
description Hibiscus rosa-sinensis grows at many places around the globe. Hibiscus rosa-sinensis contains numerous classes of auxiliary metabolites, counting flavonoids, anthocyanins, terpenoids, steroids, polysaccharides, alkaloids, amino acids, lipids, and naphthalene group. Different kind of studies show that parts of Hibiscus rosa-sinensis plants possess antioxidant, antimicrobial, antidiabetic, antiulcer, hepatoprotective, antifertility, antigenotoxic and anti-inflammatory properties that help in treatment of many diseases. Therefore, Hibiscus rosa-sinensis plant has been identified as one of many medicinal plants that possesses various potential in therapeutic applications. Bioactive compound extraction on plants done by several methods like conventional method, Microwave Assisted Extraction (MAE) method etc. However, none of the study has been done on extraction of bioactive compounds extracted from Hibiscus rosa-sinensis leaves using Ultrasonic Assisted Extraction (UAE) method. Hence, there is essential to investigate on the benefits of the Hibiscus rosa-sinensis leaves for the future used by using UAE method for extraction. The objectives of this study are to extract bioactive compound extract from Hibiscus rosa-sinensis leaves by using ultrasonic water bath using different solvents like ethanol, methanol, ethyl acetate and acetone and to analyse phenolic profile from the extract. The solvent is chosen based on the polarity due to the presence of hydroxyl group in determining the phenolic compound. Hibiscus rosa-sinensis leaves is prepared by drying at 50°C and pestled before extraction by different solvent. After that, the sample is placed in ultrasonic water bath at different time (5 min, 15 min, 30 min, 45 min, 60 min) and then the solvent is removed by using rotary evaporator. The result shows the highest extraction yield by UAE method is methanol and then followed by ethanol, acetone, and ethyl acetate. After extraction, UV-VIS spectrophotometer was used to determine the phenolic content in Hibiscus rosa-sinensis leaves. Even though methanol has the highest yield of extraction, it is not the best solvent to determine the phenolic content when comparing it with standard calibration curve of Gallic acid. However, as for ethyl acetate, it has the lowest extraction yield but able to determine the phenolic content. As a conclusion, the yield of extraction does not affect the determination of the phenolic content.
format Conference or Workshop Item
author Abdul Ghani, Nor Fazlina
Ismail, Nor Fariza
author_facet Abdul Ghani, Nor Fazlina
Ismail, Nor Fariza
author_sort Abdul Ghani, Nor Fazlina
title Extraction of Hibiscus rosa sinensis leaves using ultrasonic water bath in determining the phenolic content / Nor Fazlina Abdul Ghani and Dr Nor Fariza Ismail
title_short Extraction of Hibiscus rosa sinensis leaves using ultrasonic water bath in determining the phenolic content / Nor Fazlina Abdul Ghani and Dr Nor Fariza Ismail
title_full Extraction of Hibiscus rosa sinensis leaves using ultrasonic water bath in determining the phenolic content / Nor Fazlina Abdul Ghani and Dr Nor Fariza Ismail
title_fullStr Extraction of Hibiscus rosa sinensis leaves using ultrasonic water bath in determining the phenolic content / Nor Fazlina Abdul Ghani and Dr Nor Fariza Ismail
title_full_unstemmed Extraction of Hibiscus rosa sinensis leaves using ultrasonic water bath in determining the phenolic content / Nor Fazlina Abdul Ghani and Dr Nor Fariza Ismail
title_sort extraction of hibiscus rosa sinensis leaves using ultrasonic water bath in determining the phenolic content / nor fazlina abdul ghani and dr nor fariza ismail
publishDate 2021
url https://ir.uitm.edu.my/id/eprint/78947/1/78947.pdf
https://ir.uitm.edu.my/id/eprint/78947/
_version_ 1769846613358936064
score 13.211869

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