Development of a thermostabilised PCR-based detection kit for pathogenic familial hypercholesterolaemia variants in Malaysia / Norhidayah Rosman
Familial hypercholesterolaemia (FH) is an inherited disease that causes an elevation of plasma low-density lipoprotein cholesterol (LDL-C) level, leading to increased risk of premature coronary artery disease. Next-generation sequencing (NGS) is currently used to detect FH variants molecularly among...
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| Format: | Thesis |
| Language: | English |
| Published: |
2022
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| Subjects: | |
| Online Access: | https://ir.uitm.edu.my/id/eprint/77969/1/77969.pdf https://ir.uitm.edu.my/id/eprint/77969/ |
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| Summary: | Familial hypercholesterolaemia (FH) is an inherited disease that causes an elevation of plasma low-density lipoprotein cholesterol (LDL-C) level, leading to increased risk of premature coronary artery disease. Next-generation sequencing (NGS) is currently used to detect FH variants molecularly among patients. However, this method is expensive, laborious, and time-consuming. Thus, a simpler method using a tetra-primer samplification refractory mutation system (T-ARMS) PCR was developed for detection of 10 most common pathogenic variants in Malaysia. The kit was designed to detect 9pathogenic variants of the LDLR gene and 1 APOB gene pathogenic variant. The sevariants were selected by analysing their pathogenicity and their frequency among molecularly confirmed FH cases from previous published and unpublished data. The ratio of inner and outer primers’ concentration of each variant and the annealing temperature were optimised to achieve optimal results. The optimised PCR was then evaluated with 154 clinical samples to determine the diagnostic performance of this kit.Limit of detection (LoD) was performed using synthetic DNA targets as well as extracted patient DNA. The diagnostic performance of the kit showed 100% for sensitivity, specificity PPV, NPV and accuracy. The LoD was 1.0X10-2 ng for syntheticDNA and 10.0 ng for the extracted DNA from FH and non-FH patients. A prototype was developed by using a 96-well PCR plate with lyophilised primers of each variant dispensed into different wells. The stability of the prototype was analysed using the Q10accelerated aging method. This method showed the kit was stable at room temperature for up to three months. This thermostabilised T-ARMS PCR prototype provides a simple-to-use kit that can be performed using a simple PCR thermocycler for the rapid screening of pathogenic FH variants. It may also be useful for molecular confirmation of FH zygosity in the regional Asian countries. Easy identification of pathogenic FHvariants will allow prompt and early intervention, thus reducing the risk of coronary artery disease among the population. |
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