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Cytotoxicity and DNA cleavage studies of salicylhydroxamic acid and its Ni(II), Cu(II) and Zn(II) complexes / Nor Haslini Wahid

Ni((II), Cu(II) and Zn(II) complexes of salicylhydroxamic acid have been synthesized and characterized by elemental analysis, FT-IR, 1HNMR spectroscopy, magnetic moment, melting point determination and conductivity measurement data. The spectral data suggests that the salicylhydroxamic acid act a...

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Main Author: Wahid, Nor Haslini
Format: Student Project
Language:English
Published: Faculty of Applied Sciences 2009
Subjects:
QD Chemistry
Online Access:http://ir.uitm.edu.my/id/eprint/763/1/PPb_NOR%20HASLINI%20WAHID%20AS%2009_5%20P01.pdf
http://ir.uitm.edu.my/id/eprint/763/
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spelling my.uitm.ir.7632018-09-20T04:43:23Z http://ir.uitm.edu.my/id/eprint/763/ Cytotoxicity and DNA cleavage studies of salicylhydroxamic acid and its Ni(II), Cu(II) and Zn(II) complexes / Nor Haslini Wahid Wahid, Nor Haslini QD Chemistry Ni((II), Cu(II) and Zn(II) complexes of salicylhydroxamic acid have been synthesized and characterized by elemental analysis, FT-IR, 1HNMR spectroscopy, magnetic moment, melting point determination and conductivity measurement data. The spectral data suggests that the salicylhydroxamic acid act as a bidentate ligand and is coordinated to the metal ion through the hydroxamate group. The interaction of the salicylhydroxamic acid ligand and its complexes with double stranded supercoiled DNA was studied using agarose gel electrophoresis. From the DNA cleavage studies, it was found that the supercoiled structure of the bacterial DNA was maintained indicating that all compounds had no negative effect on bacterial DNA. The ligand and complexes were also subjected to in vitro cytotoxicity test using Chang liver cells. Treatment of Zn(II) complex to these cells have shown that the IC50 value is at 62 μM. This shows that Zn(II) complex had toxic effect on Chang liver cells. A decrease in cells viability indicates that the cells were stopping actively growing and dividing. But the ligand and other complexes tested were found to be non-toxic. Faculty of Applied Sciences 2009 Student Project NonPeerReviewed text en http://ir.uitm.edu.my/id/eprint/763/1/PPb_NOR%20HASLINI%20WAHID%20AS%2009_5%20P01.pdf Wahid, Nor Haslini (2009) Cytotoxicity and DNA cleavage studies of salicylhydroxamic acid and its Ni(II), Cu(II) and Zn(II) complexes / Nor Haslini Wahid. [Student Project] (Unpublished)
institution Universiti Teknologi Mara
building Tun Abdul Razak Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Teknologi Mara
content_source UiTM Institutional Repository
url_provider http://ir.uitm.edu.my/
language English
topic QD Chemistry
spellingShingle QD Chemistry
Wahid, Nor Haslini
Cytotoxicity and DNA cleavage studies of salicylhydroxamic acid and its Ni(II), Cu(II) and Zn(II) complexes / Nor Haslini Wahid
description Ni((II), Cu(II) and Zn(II) complexes of salicylhydroxamic acid have been synthesized and characterized by elemental analysis, FT-IR, 1HNMR spectroscopy, magnetic moment, melting point determination and conductivity measurement data. The spectral data suggests that the salicylhydroxamic acid act as a bidentate ligand and is coordinated to the metal ion through the hydroxamate group. The interaction of the salicylhydroxamic acid ligand and its complexes with double stranded supercoiled DNA was studied using agarose gel electrophoresis. From the DNA cleavage studies, it was found that the supercoiled structure of the bacterial DNA was maintained indicating that all compounds had no negative effect on bacterial DNA. The ligand and complexes were also subjected to in vitro cytotoxicity test using Chang liver cells. Treatment of Zn(II) complex to these cells have shown that the IC50 value is at 62 μM. This shows that Zn(II) complex had toxic effect on Chang liver cells. A decrease in cells viability indicates that the cells were stopping actively growing and dividing. But the ligand and other complexes tested were found to be non-toxic.
format Student Project
author Wahid, Nor Haslini
author_facet Wahid, Nor Haslini
author_sort Wahid, Nor Haslini
title Cytotoxicity and DNA cleavage studies of salicylhydroxamic acid and its Ni(II), Cu(II) and Zn(II) complexes / Nor Haslini Wahid
title_short Cytotoxicity and DNA cleavage studies of salicylhydroxamic acid and its Ni(II), Cu(II) and Zn(II) complexes / Nor Haslini Wahid
title_full Cytotoxicity and DNA cleavage studies of salicylhydroxamic acid and its Ni(II), Cu(II) and Zn(II) complexes / Nor Haslini Wahid
title_fullStr Cytotoxicity and DNA cleavage studies of salicylhydroxamic acid and its Ni(II), Cu(II) and Zn(II) complexes / Nor Haslini Wahid
title_full_unstemmed Cytotoxicity and DNA cleavage studies of salicylhydroxamic acid and its Ni(II), Cu(II) and Zn(II) complexes / Nor Haslini Wahid
title_sort cytotoxicity and dna cleavage studies of salicylhydroxamic acid and its ni(ii), cu(ii) and zn(ii) complexes / nor haslini wahid
publisher Faculty of Applied Sciences
publishDate 2009
url http://ir.uitm.edu.my/id/eprint/763/1/PPb_NOR%20HASLINI%20WAHID%20AS%2009_5%20P01.pdf
http://ir.uitm.edu.my/id/eprint/763/
_version_ 1685648089070698496
score 13.211869

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