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The preventive effect to tocotrienol on delayed preimplantation embryonic development in aging mice / Nur Aida Dalilah Mohamed Almi

Oxidative stress induced by the aging process was known to cause impairment in female reproductive and antioxidant defense system. Tocotrienol (TCT), through its antioxidant properties has been suggested to play a role in reducing oxidative stress and promoting fertility. The present study evaluates...

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Main Author: Mohamed Almi, Nur Aida Dalilah
Format: Thesis
Language:English
Published: 2013
Subjects:
QL Zoology
Tocotrienol. Tocopherol. Vitamin E
Online Access:https://ir.uitm.edu.my/id/eprint/72181/1/72181.pdf
https://ir.uitm.edu.my/id/eprint/72181/
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spelling my.uitm.ir.721812024-01-03T08:13:40Z https://ir.uitm.edu.my/id/eprint/72181/ The preventive effect to tocotrienol on delayed preimplantation embryonic development in aging mice / Nur Aida Dalilah Mohamed Almi Mohamed Almi, Nur Aida Dalilah QL Zoology Tocotrienol. Tocopherol. Vitamin E Oxidative stress induced by the aging process was known to cause impairment in female reproductive and antioxidant defense system. Tocotrienol (TCT), through its antioxidant properties has been suggested to play a role in reducing oxidative stress and promoting fertility. The present study evaluates the protective effect of TCT on the ovarian function which was assessed from the response for ovulation process, the quality and developmental competence of embryos produced, and the activity of catalase (CAT), a component of antioxidant defense system. Twelve weeks old female mice were subjected to daily oral gavages of either corn oil (vehicle) or TCT at different doses of 60, 120 and 240 mg kg _1 body weight (BW) for 8 weeks duration. Age control groups consisted of young mice (7 weeks old) and aging mice (20 weeks old). Animals were then, superovulated and cohabited with a fertile male at a ratio of 1:1. After 48 hours, animals were euthanized, the fallopian tubes were excised and embryos were flushed out. The embryos recovered were then be counted and graded based on its morphology. Only normal embryos were cultured to observe the developmental competence from 2 cells to expanded blastocyst stage. Counting the total number of embryos recovered quantified the ovarian response for ovulation process whereby grading the embryo morphology determined the embryo quality. We found out that the age related decline of embryo count, quality and in vitro development from 2 cells to 4 cells stage was improved with TCT treatment at the dose of 120 and 240 mg kg'1 BW. The embryos recovered from 240 mg kg"1 BW TCT treatment group also denoted to have a higher progression rate at 4 cells to morula stage when compared with all groups. There is no significant difference in CAT level in plasma and ovarian tissue samples in all TCT treatment groups as compared to aging control. Therefore, TCT is found to have a beneficial effect on the ovulation response, embryonic quality and in vitro development from 2 cells to morula stage in aging mice. 2013 Thesis NonPeerReviewed text en https://ir.uitm.edu.my/id/eprint/72181/1/72181.pdf The preventive effect to tocotrienol on delayed preimplantation embryonic development in aging mice / Nur Aida Dalilah Mohamed Almi. (2013) Degree thesis, thesis, Universiti Teknologi MARA (UiTM).
institution Universiti Teknologi Mara
building Tun Abdul Razak Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Teknologi Mara
content_source UiTM Institutional Repository
url_provider http://ir.uitm.edu.my/
language English
topic QL Zoology
Tocotrienol. Tocopherol. Vitamin E
spellingShingle QL Zoology
Tocotrienol. Tocopherol. Vitamin E
Mohamed Almi, Nur Aida Dalilah
The preventive effect to tocotrienol on delayed preimplantation embryonic development in aging mice / Nur Aida Dalilah Mohamed Almi
description Oxidative stress induced by the aging process was known to cause impairment in female reproductive and antioxidant defense system. Tocotrienol (TCT), through its antioxidant properties has been suggested to play a role in reducing oxidative stress and promoting fertility. The present study evaluates the protective effect of TCT on the ovarian function which was assessed from the response for ovulation process, the quality and developmental competence of embryos produced, and the activity of catalase (CAT), a component of antioxidant defense system. Twelve weeks old female mice were subjected to daily oral gavages of either corn oil (vehicle) or TCT at different doses of 60, 120 and 240 mg kg _1 body weight (BW) for 8 weeks duration. Age control groups consisted of young mice (7 weeks old) and aging mice (20 weeks old). Animals were then, superovulated and cohabited with a fertile male at a ratio of 1:1. After 48 hours, animals were euthanized, the fallopian tubes were excised and embryos were flushed out. The embryos recovered were then be counted and graded based on its morphology. Only normal embryos were cultured to observe the developmental competence from 2 cells to expanded blastocyst stage. Counting the total number of embryos recovered quantified the ovarian response for ovulation process whereby grading the embryo morphology determined the embryo quality. We found out that the age related decline of embryo count, quality and in vitro development from 2 cells to 4 cells stage was improved with TCT treatment at the dose of 120 and 240 mg kg'1 BW. The embryos recovered from 240 mg kg"1 BW TCT treatment group also denoted to have a higher progression rate at 4 cells to morula stage when compared with all groups. There is no significant difference in CAT level in plasma and ovarian tissue samples in all TCT treatment groups as compared to aging control. Therefore, TCT is found to have a beneficial effect on the ovulation response, embryonic quality and in vitro development from 2 cells to morula stage in aging mice.
format Thesis
author Mohamed Almi, Nur Aida Dalilah
author_facet Mohamed Almi, Nur Aida Dalilah
author_sort Mohamed Almi, Nur Aida Dalilah
title The preventive effect to tocotrienol on delayed preimplantation embryonic development in aging mice / Nur Aida Dalilah Mohamed Almi
title_short The preventive effect to tocotrienol on delayed preimplantation embryonic development in aging mice / Nur Aida Dalilah Mohamed Almi
title_full The preventive effect to tocotrienol on delayed preimplantation embryonic development in aging mice / Nur Aida Dalilah Mohamed Almi
title_fullStr The preventive effect to tocotrienol on delayed preimplantation embryonic development in aging mice / Nur Aida Dalilah Mohamed Almi
title_full_unstemmed The preventive effect to tocotrienol on delayed preimplantation embryonic development in aging mice / Nur Aida Dalilah Mohamed Almi
title_sort preventive effect to tocotrienol on delayed preimplantation embryonic development in aging mice / nur aida dalilah mohamed almi
publishDate 2013
url https://ir.uitm.edu.my/id/eprint/72181/1/72181.pdf
https://ir.uitm.edu.my/id/eprint/72181/
_version_ 1787139493765054464
score 13.211869

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