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Aloe emodin regulates the suppression of breast cancer cell proliferation via calcium signalling / Mohd Ridzuan Hamid

Deregulation of Ca2+ signaling is one of the characteristics of breast cancer cells. Our preliminary findings have demonstrated antiproliferative effects of aloe emodin (AE), anthraquinone extract of Aloe barbadensis Miller on breast cancer cells, MCF-7. However, the influence of Ca2+ regulation in...

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Main Author: Hamid, Mohd Ridzuan
Format: Thesis
Language:English
Published: 2012
Subjects:
Cancer
Online Access:https://ir.uitm.edu.my/id/eprint/72174/1/72174.pdf
https://ir.uitm.edu.my/id/eprint/72174/
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spelling my.uitm.ir.721742024-01-02T09:04:28Z https://ir.uitm.edu.my/id/eprint/72174/ Aloe emodin regulates the suppression of breast cancer cell proliferation via calcium signalling / Mohd Ridzuan Hamid Hamid, Mohd Ridzuan Cancer Deregulation of Ca2+ signaling is one of the characteristics of breast cancer cells. Our preliminary findings have demonstrated antiproliferative effects of aloe emodin (AE), anthraquinone extract of Aloe barbadensis Miller on breast cancer cells, MCF-7. However, the influence of Ca2+ regulation in AE-treated breast cancer cells is not well known. Therefore, this study was done to discover the intracellular calcium dynamics of AE-suppressed proliferation in breast cancer cells, MCF-7. Cell viability after AE treatment for 1, 6 and 12 hours on MCF-7 cells were studied using trypan blue exclusion method. Intracellular calcium study was done by loading the MCF-7 cells with calcium specific fluorescent dye Fluo4-AM for 45 minutes and the fluo4 intensity changes over time were recorded using confocal laser scanning microscope. Fluorescence intensity was measured for untreated cells, Thapsigargin (TG) and AE treated cells. TG was used as positive control in this study as it is known to result in high cytosolic Ca2+ dynamics. Throughout cell viability determination, there is no significant cell death for all time points. 2012 Thesis NonPeerReviewed text en https://ir.uitm.edu.my/id/eprint/72174/1/72174.pdf Aloe emodin regulates the suppression of breast cancer cell proliferation via calcium signalling / Mohd Ridzuan Hamid. (2012) Degree thesis, thesis, Universiti Teknologi MARA (UiTM).
institution Universiti Teknologi Mara
building Tun Abdul Razak Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Teknologi Mara
content_source UiTM Institutional Repository
url_provider http://ir.uitm.edu.my/
language English
topic Cancer
spellingShingle Cancer
Hamid, Mohd Ridzuan
Aloe emodin regulates the suppression of breast cancer cell proliferation via calcium signalling / Mohd Ridzuan Hamid
description Deregulation of Ca2+ signaling is one of the characteristics of breast cancer cells. Our preliminary findings have demonstrated antiproliferative effects of aloe emodin (AE), anthraquinone extract of Aloe barbadensis Miller on breast cancer cells, MCF-7. However, the influence of Ca2+ regulation in AE-treated breast cancer cells is not well known. Therefore, this study was done to discover the intracellular calcium dynamics of AE-suppressed proliferation in breast cancer cells, MCF-7. Cell viability after AE treatment for 1, 6 and 12 hours on MCF-7 cells were studied using trypan blue exclusion method. Intracellular calcium study was done by loading the MCF-7 cells with calcium specific fluorescent dye Fluo4-AM for 45 minutes and the fluo4 intensity changes over time were recorded using confocal laser scanning microscope. Fluorescence intensity was measured for untreated cells, Thapsigargin (TG) and AE treated cells. TG was used as positive control in this study as it is known to result in high cytosolic Ca2+ dynamics. Throughout cell viability determination, there is no significant cell death for all time points.
format Thesis
author Hamid, Mohd Ridzuan
author_facet Hamid, Mohd Ridzuan
author_sort Hamid, Mohd Ridzuan
title Aloe emodin regulates the suppression of breast cancer cell proliferation via calcium signalling / Mohd Ridzuan Hamid
title_short Aloe emodin regulates the suppression of breast cancer cell proliferation via calcium signalling / Mohd Ridzuan Hamid
title_full Aloe emodin regulates the suppression of breast cancer cell proliferation via calcium signalling / Mohd Ridzuan Hamid
title_fullStr Aloe emodin regulates the suppression of breast cancer cell proliferation via calcium signalling / Mohd Ridzuan Hamid
title_full_unstemmed Aloe emodin regulates the suppression of breast cancer cell proliferation via calcium signalling / Mohd Ridzuan Hamid
title_sort aloe emodin regulates the suppression of breast cancer cell proliferation via calcium signalling / mohd ridzuan hamid
publishDate 2012
url https://ir.uitm.edu.my/id/eprint/72174/1/72174.pdf
https://ir.uitm.edu.my/id/eprint/72174/
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score 13.211869

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