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Antimicrobial activity of Malaysian honeys against Escherichia coli and Bacillus subtilis / Muhammad Affiq Muhammad

The antibacterial activity of honey against pathogens of the gastrointestinal system has been widely studied. However, not many reports considered the efficacy of the Malaysian honey against enteropathogenic species. Thus, this study is designed to understand the potential impact of the antibacteria...

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Main Author: Muhammad, Muhammad Affiq
Format: Thesis
Language:English
Published: 2012
Subjects:
Antibacterial agents
Online Access:https://ir.uitm.edu.my/id/eprint/66694/1/66694.pdf
https://ir.uitm.edu.my/id/eprint/66694/
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spelling my.uitm.ir.666942022-09-14T02:40:22Z https://ir.uitm.edu.my/id/eprint/66694/ Antimicrobial activity of Malaysian honeys against Escherichia coli and Bacillus subtilis / Muhammad Affiq Muhammad Muhammad, Muhammad Affiq Antibacterial agents The antibacterial activity of honey against pathogens of the gastrointestinal system has been widely studied. However, not many reports considered the efficacy of the Malaysian honey against enteropathogenic species. Thus, this study is designed to understand the potential impact of the antibacterial activity of selected Malaysian honey in comparison with Manuka honey on the pathogens of the digestive system. Five honey samples (Gelam, Tualang, Nenas, Acacia and Manuka) with 8 different concentrations (3 - 45 %) (v/v) were tested against E. coli and B. subtilis using broth dilution method. Minimum inhibitory concentration (MIC) of honey was determined by visual inspection after incubated for 24 hour. Minimum bactericidal concentration (MBC) also was determined by culturing on agar plates and incubated for 24 hour. The results showed that the MIC of Manuka honey is 9 %, Gelam and Acacia (21 %) while Nenas and Tualang (27 %) against E. coli. The MIC of Manuka honey is 15 %, Gelam and Tualang (21 %) while Nenas and Acacia (27 %) against B. subtilis. Selected Malaysian honeys exhibited variable antibacterial activity against E. coli and B. subtilis species with Gelam honey demonstrated good antibacterial activity against both bacteria. However, selected Malaysian honeys have less efficacy as compare to the proprietary Manuka honey in modulating E. coli and B. subtilis species. Although our local honey may not be as good as Manuka honey, further research is required to assess the true potential of these honeys. 2012 Thesis NonPeerReviewed text en https://ir.uitm.edu.my/id/eprint/66694/1/66694.pdf Antimicrobial activity of Malaysian honeys against Escherichia coli and Bacillus subtilis / Muhammad Affiq Muhammad. (2012) Degree thesis, thesis, Universiti Teknologi MARA (UiTM). <http://terminalib.uitm.edu.my/66694.pdf>
institution Universiti Teknologi Mara
building Tun Abdul Razak Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Teknologi Mara
content_source UiTM Institutional Repository
url_provider http://ir.uitm.edu.my/
language English
topic Antibacterial agents
spellingShingle Antibacterial agents
Muhammad, Muhammad Affiq
Antimicrobial activity of Malaysian honeys against Escherichia coli and Bacillus subtilis / Muhammad Affiq Muhammad
description The antibacterial activity of honey against pathogens of the gastrointestinal system has been widely studied. However, not many reports considered the efficacy of the Malaysian honey against enteropathogenic species. Thus, this study is designed to understand the potential impact of the antibacterial activity of selected Malaysian honey in comparison with Manuka honey on the pathogens of the digestive system. Five honey samples (Gelam, Tualang, Nenas, Acacia and Manuka) with 8 different concentrations (3 - 45 %) (v/v) were tested against E. coli and B. subtilis using broth dilution method. Minimum inhibitory concentration (MIC) of honey was determined by visual inspection after incubated for 24 hour. Minimum bactericidal concentration (MBC) also was determined by culturing on agar plates and incubated for 24 hour. The results showed that the MIC of Manuka honey is 9 %, Gelam and Acacia (21 %) while Nenas and Tualang (27 %) against E. coli. The MIC of Manuka honey is 15 %, Gelam and Tualang (21 %) while Nenas and Acacia (27 %) against B. subtilis. Selected Malaysian honeys exhibited variable antibacterial activity against E. coli and B. subtilis species with Gelam honey demonstrated good antibacterial activity against both bacteria. However, selected Malaysian honeys have less efficacy as compare to the proprietary Manuka honey in modulating E. coli and B. subtilis species. Although our local honey may not be as good as Manuka honey, further research is required to assess the true potential of these honeys.
format Thesis
author Muhammad, Muhammad Affiq
author_facet Muhammad, Muhammad Affiq
author_sort Muhammad, Muhammad Affiq
title Antimicrobial activity of Malaysian honeys against Escherichia coli and Bacillus subtilis / Muhammad Affiq Muhammad
title_short Antimicrobial activity of Malaysian honeys against Escherichia coli and Bacillus subtilis / Muhammad Affiq Muhammad
title_full Antimicrobial activity of Malaysian honeys against Escherichia coli and Bacillus subtilis / Muhammad Affiq Muhammad
title_fullStr Antimicrobial activity of Malaysian honeys against Escherichia coli and Bacillus subtilis / Muhammad Affiq Muhammad
title_full_unstemmed Antimicrobial activity of Malaysian honeys against Escherichia coli and Bacillus subtilis / Muhammad Affiq Muhammad
title_sort antimicrobial activity of malaysian honeys against escherichia coli and bacillus subtilis / muhammad affiq muhammad
publishDate 2012
url https://ir.uitm.edu.my/id/eprint/66694/1/66694.pdf
https://ir.uitm.edu.my/id/eprint/66694/
_version_ 1744357291868553216
score 13.211869

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