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Determination cytotoxicity of myricetin and mahanimbine on human tumor glioma cell lines, SNB-19 and SNB-75 / Hosni Azhar

Myricetin and mahanimbine widely used as anti-oxidant. Both compounds have been found potential as an anti-cancer properties against cancer cell such as Human Colon Carcinoma and Human Leukemic cell lines. However the effect of compouds againts human glioma cells (SNB-19 and SNB-75) have not been re...

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Main Author: Azhar, Hosni
Format: Thesis
Language:English
Published: 2012
Subjects:
Cancer
Research. Experimentation
Online Access:https://ir.uitm.edu.my/id/eprint/66679/1/66679.pdf
https://ir.uitm.edu.my/id/eprint/66679/
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spelling my.uitm.ir.666792022-09-14T01:39:51Z https://ir.uitm.edu.my/id/eprint/66679/ Determination cytotoxicity of myricetin and mahanimbine on human tumor glioma cell lines, SNB-19 and SNB-75 / Hosni Azhar Azhar, Hosni Cancer Research. Experimentation Myricetin and mahanimbine widely used as anti-oxidant. Both compounds have been found potential as an anti-cancer properties against cancer cell such as Human Colon Carcinoma and Human Leukemic cell lines. However the effect of compouds againts human glioma cells (SNB-19 and SNB-75) have not been reported. In this study, the cytotoxic activity of myricetin and mahanimbine was investigate. MTT assay was used to measure the cytotoxic activity. Human tumor glioma cell line SNB-19 and SNB-75 was cultured in the optimum environment which is using Minimal Essential Medium (MEM) with Earle's Salts media and the conditions of culture were 37°C and 5% C02. Cell were plated into 96-flat bottom well plate with a density of 5000 cells/well and incubated for 24 hours. After incubation, the plated cell were treated with with 20uL of of test compounds at various concentrations then incubated for 72 hours. MTT solution was added and read using the ELISA® micro plate reader. IC50 (value of cytotoxic activity) were derived from dose-response curve of which the concentration of extracts required to kill 50% of cell population. Result showed that mahanimbine had IC50 values of 16.56ug/ml on SNB-19 and 7.07ug/ml on SNB-75 while myricetin exhibited cytotoxic activity with IC50 values of 22.11 ug/ml on SNB- 19 and 28.44ug/ml on SNB-75. Both compounds had an inhibitory effect on cancer cells and normal cells indicating that test compounds did not show selectivity. Further investigation to improve the selectivity should established for effective and safe therapy. 2012 Thesis NonPeerReviewed text en https://ir.uitm.edu.my/id/eprint/66679/1/66679.pdf Determination cytotoxicity of myricetin and mahanimbine on human tumor glioma cell lines, SNB-19 and SNB-75 / Hosni Azhar. (2012) Degree thesis, thesis, Universiti Teknologi MARA (UiTM). <http://terminalib.uitm.edu.my/66679.pdf>
institution Universiti Teknologi Mara
building Tun Abdul Razak Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Teknologi Mara
content_source UiTM Institutional Repository
url_provider http://ir.uitm.edu.my/
language English
topic Cancer
Research. Experimentation
spellingShingle Cancer
Research. Experimentation
Azhar, Hosni
Determination cytotoxicity of myricetin and mahanimbine on human tumor glioma cell lines, SNB-19 and SNB-75 / Hosni Azhar
description Myricetin and mahanimbine widely used as anti-oxidant. Both compounds have been found potential as an anti-cancer properties against cancer cell such as Human Colon Carcinoma and Human Leukemic cell lines. However the effect of compouds againts human glioma cells (SNB-19 and SNB-75) have not been reported. In this study, the cytotoxic activity of myricetin and mahanimbine was investigate. MTT assay was used to measure the cytotoxic activity. Human tumor glioma cell line SNB-19 and SNB-75 was cultured in the optimum environment which is using Minimal Essential Medium (MEM) with Earle's Salts media and the conditions of culture were 37°C and 5% C02. Cell were plated into 96-flat bottom well plate with a density of 5000 cells/well and incubated for 24 hours. After incubation, the plated cell were treated with with 20uL of of test compounds at various concentrations then incubated for 72 hours. MTT solution was added and read using the ELISA® micro plate reader. IC50 (value of cytotoxic activity) were derived from dose-response curve of which the concentration of extracts required to kill 50% of cell population. Result showed that mahanimbine had IC50 values of 16.56ug/ml on SNB-19 and 7.07ug/ml on SNB-75 while myricetin exhibited cytotoxic activity with IC50 values of 22.11 ug/ml on SNB- 19 and 28.44ug/ml on SNB-75. Both compounds had an inhibitory effect on cancer cells and normal cells indicating that test compounds did not show selectivity. Further investigation to improve the selectivity should established for effective and safe therapy.
format Thesis
author Azhar, Hosni
author_facet Azhar, Hosni
author_sort Azhar, Hosni
title Determination cytotoxicity of myricetin and mahanimbine on human tumor glioma cell lines, SNB-19 and SNB-75 / Hosni Azhar
title_short Determination cytotoxicity of myricetin and mahanimbine on human tumor glioma cell lines, SNB-19 and SNB-75 / Hosni Azhar
title_full Determination cytotoxicity of myricetin and mahanimbine on human tumor glioma cell lines, SNB-19 and SNB-75 / Hosni Azhar
title_fullStr Determination cytotoxicity of myricetin and mahanimbine on human tumor glioma cell lines, SNB-19 and SNB-75 / Hosni Azhar
title_full_unstemmed Determination cytotoxicity of myricetin and mahanimbine on human tumor glioma cell lines, SNB-19 and SNB-75 / Hosni Azhar
title_sort determination cytotoxicity of myricetin and mahanimbine on human tumor glioma cell lines, snb-19 and snb-75 / hosni azhar
publishDate 2012
url https://ir.uitm.edu.my/id/eprint/66679/1/66679.pdf
https://ir.uitm.edu.my/id/eprint/66679/
_version_ 1744357290768596992
score 13.211869

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