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Docking of mouse P-glycoprotein (ABCB1a/MDR3) with verapamil and cyclosporine A / Zakiah Salleh

Background: Nowadays, P-glycoprotein becomes the main focus of research interest since its discovery in 1976 by Juliano and Ling in Chinese hamster ovary cells. P-Glycoprotein (Pgp) is identified from adenosine triphosphate (ATP)-binding cassette (ABC) superfamily that encoding for gene in human of...

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Main Author: Salleh, Zakiah
Format: Thesis
Language:English
Published: 2012
Subjects:
Genetics
Cancer
Online Access:https://ir.uitm.edu.my/id/eprint/65622/1/65622.pdf
https://ir.uitm.edu.my/id/eprint/65622/
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spelling my.uitm.ir.656222022-08-24T02:21:45Z https://ir.uitm.edu.my/id/eprint/65622/ Docking of mouse P-glycoprotein (ABCB1a/MDR3) with verapamil and cyclosporine A / Zakiah Salleh Salleh, Zakiah Genetics Cancer Background: Nowadays, P-glycoprotein becomes the main focus of research interest since its discovery in 1976 by Juliano and Ling in Chinese hamster ovary cells. P-Glycoprotein (Pgp) is identified from adenosine triphosphate (ATP)-binding cassette (ABC) superfamily that encoding for gene in human of ABCB1. It can be identified as 3G5U from Mus musculus (mouse). Result: The amino acid residues that having high binding affinity toward Pgp are Leu335 and Ala338 at TM 6. At TM 12, the amino acid residues like Val981, Val978 and Gly980 are consider the success amino acid that bind to Pgp. Then, the Pgp was docked using cyclosporine A as a ligand and was finding that the amino acid residue that binds to Pgp is only Ala338. Meanwhile, at TM 12, the amino acid residues that success binds to Pgp are Phe974 and Val978. Meanwhile, at TM 7, there is one amino acid residue success bind to Pgp which is Phe728 and Phe724. Conclusion: The active binding sites of Pgp are at TM 6, TM 7 and TM 12. AutoDock software can be responsible for in silico method in designing the drug specificity in order to prevent multi drug resistance (MDR) especially in cancer cell. 2012 Thesis NonPeerReviewed text en https://ir.uitm.edu.my/id/eprint/65622/1/65622.pdf Docking of mouse P-glycoprotein (ABCB1a/MDR3) with verapamil and cyclosporine A / Zakiah Salleh. (2012) Degree thesis, thesis, Universiti Teknologi MARA (UiTM). <http://terminalib.uitm.edu.my/65622.pdf>
institution Universiti Teknologi Mara
building Tun Abdul Razak Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Teknologi Mara
content_source UiTM Institutional Repository
url_provider http://ir.uitm.edu.my/
language English
topic Genetics
Cancer
spellingShingle Genetics
Cancer
Salleh, Zakiah
Docking of mouse P-glycoprotein (ABCB1a/MDR3) with verapamil and cyclosporine A / Zakiah Salleh
description Background: Nowadays, P-glycoprotein becomes the main focus of research interest since its discovery in 1976 by Juliano and Ling in Chinese hamster ovary cells. P-Glycoprotein (Pgp) is identified from adenosine triphosphate (ATP)-binding cassette (ABC) superfamily that encoding for gene in human of ABCB1. It can be identified as 3G5U from Mus musculus (mouse). Result: The amino acid residues that having high binding affinity toward Pgp are Leu335 and Ala338 at TM 6. At TM 12, the amino acid residues like Val981, Val978 and Gly980 are consider the success amino acid that bind to Pgp. Then, the Pgp was docked using cyclosporine A as a ligand and was finding that the amino acid residue that binds to Pgp is only Ala338. Meanwhile, at TM 12, the amino acid residues that success binds to Pgp are Phe974 and Val978. Meanwhile, at TM 7, there is one amino acid residue success bind to Pgp which is Phe728 and Phe724. Conclusion: The active binding sites of Pgp are at TM 6, TM 7 and TM 12. AutoDock software can be responsible for in silico method in designing the drug specificity in order to prevent multi drug resistance (MDR) especially in cancer cell.
format Thesis
author Salleh, Zakiah
author_facet Salleh, Zakiah
author_sort Salleh, Zakiah
title Docking of mouse P-glycoprotein (ABCB1a/MDR3) with verapamil and cyclosporine A / Zakiah Salleh
title_short Docking of mouse P-glycoprotein (ABCB1a/MDR3) with verapamil and cyclosporine A / Zakiah Salleh
title_full Docking of mouse P-glycoprotein (ABCB1a/MDR3) with verapamil and cyclosporine A / Zakiah Salleh
title_fullStr Docking of mouse P-glycoprotein (ABCB1a/MDR3) with verapamil and cyclosporine A / Zakiah Salleh
title_full_unstemmed Docking of mouse P-glycoprotein (ABCB1a/MDR3) with verapamil and cyclosporine A / Zakiah Salleh
title_sort docking of mouse p-glycoprotein (abcb1a/mdr3) with verapamil and cyclosporine a / zakiah salleh
publishDate 2012
url https://ir.uitm.edu.my/id/eprint/65622/1/65622.pdf
https://ir.uitm.edu.my/id/eprint/65622/
_version_ 1743110464875790336
score 13.211869

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