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Cytotoxic activity of methanolic extract of Terminalia catappa. leaves toward MCF-7 breast cancer cell lines / Mohammad Fikkiruddin Jamsari

Terminalia catappa .L extract was popular among folk Chinese Medicine and has several proven activities as antioxidant, anticancer, antimicrobial and antinflammatory effect. In this study cytotoxic effect of two different methanolic extract of Terminalia catappa .L which are green and yellow were t...

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Main Author: Jamsari, Mohammad Fikkiruddin
Format: Thesis
Language:English
Published: 2012
Subjects:
Research. Experimentation
Online Access:https://ir.uitm.edu.my/id/eprint/65560/1/65560.pdf
https://ir.uitm.edu.my/id/eprint/65560/
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spelling my.uitm.ir.655602022-08-23T02:29:20Z https://ir.uitm.edu.my/id/eprint/65560/ Cytotoxic activity of methanolic extract of Terminalia catappa. leaves toward MCF-7 breast cancer cell lines / Mohammad Fikkiruddin Jamsari Jamsari, Mohammad Fikkiruddin Research. Experimentation Terminalia catappa .L extract was popular among folk Chinese Medicine and has several proven activities as antioxidant, anticancer, antimicrobial and antinflammatory effect. In this study cytotoxic effect of two different methanolic extract of Terminalia catappa .L which are green and yellow were tested in-vitro on positive estrogen receptor breast cancer cell line (MCF-7). By using MTT assay, MCF-7 cell line has been treated with various concentrations ranging from 10000μg/ml to lowest concentration at 24, 48 and 72 hours to evaluate the cell proliferation and viability. Methanolic extract of Terminalia catappa .L inhibited cell proliferation and reduced cell viability following the concentration and time dependent manner. Only 48 and 72 hours of treatment have shown IC50 value for both methanolic extract. Yellow methanol extract seem to be more potent than green methanol extract where the IC50 for 48 hour treatment were 1118.66 μg/ml ; 2019.57 μg/ml and 72 hours treatment were 245.54 μg/ml ; 274.46 μg/ml, respectively. Furthermore, the IC50 for each treatment had been used to assess the apoptosis feature of MCF-7 by using AO/PI staining and observed under blue light of confocal microscope. The results indicated that methanolic extract of Terminalia catappa L. have cytotoxic effect towards MCF-7 cell line. 2012 Thesis NonPeerReviewed text en https://ir.uitm.edu.my/id/eprint/65560/1/65560.pdf Cytotoxic activity of methanolic extract of Terminalia catappa. leaves toward MCF-7 breast cancer cell lines / Mohammad Fikkiruddin Jamsari. (2012) Degree thesis, thesis, Universiti Teknologi MARA (UiTM). <http://terminalib.uitm.edu.my/65560.pdf>
institution Universiti Teknologi Mara
building Tun Abdul Razak Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Teknologi Mara
content_source UiTM Institutional Repository
url_provider http://ir.uitm.edu.my/
language English
topic Research. Experimentation
spellingShingle Research. Experimentation
Jamsari, Mohammad Fikkiruddin
Cytotoxic activity of methanolic extract of Terminalia catappa. leaves toward MCF-7 breast cancer cell lines / Mohammad Fikkiruddin Jamsari
description Terminalia catappa .L extract was popular among folk Chinese Medicine and has several proven activities as antioxidant, anticancer, antimicrobial and antinflammatory effect. In this study cytotoxic effect of two different methanolic extract of Terminalia catappa .L which are green and yellow were tested in-vitro on positive estrogen receptor breast cancer cell line (MCF-7). By using MTT assay, MCF-7 cell line has been treated with various concentrations ranging from 10000μg/ml to lowest concentration at 24, 48 and 72 hours to evaluate the cell proliferation and viability. Methanolic extract of Terminalia catappa .L inhibited cell proliferation and reduced cell viability following the concentration and time dependent manner. Only 48 and 72 hours of treatment have shown IC50 value for both methanolic extract. Yellow methanol extract seem to be more potent than green methanol extract where the IC50 for 48 hour treatment were 1118.66 μg/ml ; 2019.57 μg/ml and 72 hours treatment were 245.54 μg/ml ; 274.46 μg/ml, respectively. Furthermore, the IC50 for each treatment had been used to assess the apoptosis feature of MCF-7 by using AO/PI staining and observed under blue light of confocal microscope. The results indicated that methanolic extract of Terminalia catappa L. have cytotoxic effect towards MCF-7 cell line.
format Thesis
author Jamsari, Mohammad Fikkiruddin
author_facet Jamsari, Mohammad Fikkiruddin
author_sort Jamsari, Mohammad Fikkiruddin
title Cytotoxic activity of methanolic extract of Terminalia catappa. leaves toward MCF-7 breast cancer cell lines / Mohammad Fikkiruddin Jamsari
title_short Cytotoxic activity of methanolic extract of Terminalia catappa. leaves toward MCF-7 breast cancer cell lines / Mohammad Fikkiruddin Jamsari
title_full Cytotoxic activity of methanolic extract of Terminalia catappa. leaves toward MCF-7 breast cancer cell lines / Mohammad Fikkiruddin Jamsari
title_fullStr Cytotoxic activity of methanolic extract of Terminalia catappa. leaves toward MCF-7 breast cancer cell lines / Mohammad Fikkiruddin Jamsari
title_full_unstemmed Cytotoxic activity of methanolic extract of Terminalia catappa. leaves toward MCF-7 breast cancer cell lines / Mohammad Fikkiruddin Jamsari
title_sort cytotoxic activity of methanolic extract of terminalia catappa. leaves toward mcf-7 breast cancer cell lines / mohammad fikkiruddin jamsari
publishDate 2012
url https://ir.uitm.edu.my/id/eprint/65560/1/65560.pdf
https://ir.uitm.edu.my/id/eprint/65560/
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score 13.211869

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