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Antiviral activity of N-substituted 5-(Phenylamino) uracil derivatives against chikungunya virus / Noor Farah Omar Ahmad

Chikungunya virus (CHIKV), is an arthropod-borne disease that causes Chikungunya fever. Currently, there is no available drug or vaccine to treat the infected CHIKV patients. Based on literature, novel N-substituted 5-(phenylamino)uracil derivatives exhibit inhibitory effects against HIV and Hepatit...

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Main Author: Omar Ahmad, Noor Farah
Format: Thesis
Language:English
Published: 2018
Subjects:
Communicable diseases and public health
Infectious and parasitic diseases
Online Access:https://ir.uitm.edu.my/id/eprint/63344/1/63344.pdf
https://ir.uitm.edu.my/id/eprint/63344/
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spelling my.uitm.ir.633442022-12-01T04:34:08Z https://ir.uitm.edu.my/id/eprint/63344/ Antiviral activity of N-substituted 5-(Phenylamino) uracil derivatives against chikungunya virus / Noor Farah Omar Ahmad Omar Ahmad, Noor Farah Communicable diseases and public health Infectious and parasitic diseases Chikungunya virus (CHIKV), is an arthropod-borne disease that causes Chikungunya fever. Currently, there is no available drug or vaccine to treat the infected CHIKV patients. Based on literature, novel N-substituted 5-(phenylamino)uracil derivatives exhibit inhibitory effects against HIV and Hepatitis C virus but not yet been tested on CHIKV. The half maximal cytotoxic concentration (CC₅₀) of six 5-substituted(phenylamino) uracil and five 2,4-dioxo-3,4-dihydropyrimidine acetic acid compounds were at 200 µM and 800 µM respectively. Two compounds (Z214 and Z364) exhibited the best antiviral activity at concentration of 50 μM and 100 μM. Time-addition assay revealed that the inhibition was most efficient when Z214 (50 µΜ) and Z364 (100 μM) were added at 4 hour of post-infection (hpi) and at 6 hpi. This suggests that, these compounds have inhibitory effect as anti-CHIKV inhibitors at post-entry step of CHIKV replication cycle. Prophylactic treatment showed a 50 decrease in number of CHIKV plaques when Z214 (50 µM) and Z364 (100 μM) were added 5 hours before infection by 100% and 71% ± 7.01 respectively. Z214 and Z354 exhibited a significant effect against CHIKV attachment and adsorption to the Vero cells at all tested concentrations (1.56 µM to 100 µM) as compared to the virus control. Both compounds exhibited inhibition against CHIKV internalization when the compounds (at all tested concentration ranging from 1.56 µM to 100 µM) were added during virus internalization. In conclusion, these compounds under novel Nsubstituted 5-(phenylamino)uracil derivatives exhibited promising antiviral activity for Chikungunya virus and it could be further studied. 2018-04 Thesis NonPeerReviewed text en https://ir.uitm.edu.my/id/eprint/63344/1/63344.pdf Antiviral activity of N-substituted 5-(Phenylamino) uracil derivatives against chikungunya virus / Noor Farah Omar Ahmad. (2018) Masters thesis, thesis, Universiti Teknologi MARA (Kampus Sg. Buloh). <http://terminalib.uitm.edu.my/63344.pdf>
institution Universiti Teknologi Mara
building Tun Abdul Razak Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Teknologi Mara
content_source UiTM Institutional Repository
url_provider http://ir.uitm.edu.my/
language English
topic Communicable diseases and public health
Infectious and parasitic diseases
spellingShingle Communicable diseases and public health
Infectious and parasitic diseases
Omar Ahmad, Noor Farah
Antiviral activity of N-substituted 5-(Phenylamino) uracil derivatives against chikungunya virus / Noor Farah Omar Ahmad
description Chikungunya virus (CHIKV), is an arthropod-borne disease that causes Chikungunya fever. Currently, there is no available drug or vaccine to treat the infected CHIKV patients. Based on literature, novel N-substituted 5-(phenylamino)uracil derivatives exhibit inhibitory effects against HIV and Hepatitis C virus but not yet been tested on CHIKV. The half maximal cytotoxic concentration (CC₅₀) of six 5-substituted(phenylamino) uracil and five 2,4-dioxo-3,4-dihydropyrimidine acetic acid compounds were at 200 µM and 800 µM respectively. Two compounds (Z214 and Z364) exhibited the best antiviral activity at concentration of 50 μM and 100 μM. Time-addition assay revealed that the inhibition was most efficient when Z214 (50 µΜ) and Z364 (100 μM) were added at 4 hour of post-infection (hpi) and at 6 hpi. This suggests that, these compounds have inhibitory effect as anti-CHIKV inhibitors at post-entry step of CHIKV replication cycle. Prophylactic treatment showed a 50 decrease in number of CHIKV plaques when Z214 (50 µM) and Z364 (100 μM) were added 5 hours before infection by 100% and 71% ± 7.01 respectively. Z214 and Z354 exhibited a significant effect against CHIKV attachment and adsorption to the Vero cells at all tested concentrations (1.56 µM to 100 µM) as compared to the virus control. Both compounds exhibited inhibition against CHIKV internalization when the compounds (at all tested concentration ranging from 1.56 µM to 100 µM) were added during virus internalization. In conclusion, these compounds under novel Nsubstituted 5-(phenylamino)uracil derivatives exhibited promising antiviral activity for Chikungunya virus and it could be further studied.
format Thesis
author Omar Ahmad, Noor Farah
author_facet Omar Ahmad, Noor Farah
author_sort Omar Ahmad, Noor Farah
title Antiviral activity of N-substituted 5-(Phenylamino) uracil derivatives against chikungunya virus / Noor Farah Omar Ahmad
title_short Antiviral activity of N-substituted 5-(Phenylamino) uracil derivatives against chikungunya virus / Noor Farah Omar Ahmad
title_full Antiviral activity of N-substituted 5-(Phenylamino) uracil derivatives against chikungunya virus / Noor Farah Omar Ahmad
title_fullStr Antiviral activity of N-substituted 5-(Phenylamino) uracil derivatives against chikungunya virus / Noor Farah Omar Ahmad
title_full_unstemmed Antiviral activity of N-substituted 5-(Phenylamino) uracil derivatives against chikungunya virus / Noor Farah Omar Ahmad
title_sort antiviral activity of n-substituted 5-(phenylamino) uracil derivatives against chikungunya virus / noor farah omar ahmad
publishDate 2018
url https://ir.uitm.edu.my/id/eprint/63344/1/63344.pdf
https://ir.uitm.edu.my/id/eprint/63344/
_version_ 1751539875526475776
score 13.211869

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