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Glycoprotein profiling of Staphylococcus epidermidis and its role in biofilm formation and antibiotic resistance / Nur Alia Othman

Staphylococcus epidermidis is the most frequently detected coagulase-negative staphylococcus (CoNS) that is commonly responsible for catheter-related and central nervous system (CNS) infections. It is an opportunistic pathogen capable of forming biofilm which contributes to device-related infections...

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Main Author: Othman, Nur Alia
Format: Thesis
Language:English
Published: 2021
Subjects:
Bacteria
Online Access:https://ir.uitm.edu.my/id/eprint/60522/1/60522.pdf
https://ir.uitm.edu.my/id/eprint/60522/
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spelling my.uitm.ir.605222022-05-30T03:46:33Z https://ir.uitm.edu.my/id/eprint/60522/ Glycoprotein profiling of Staphylococcus epidermidis and its role in biofilm formation and antibiotic resistance / Nur Alia Othman Othman, Nur Alia Bacteria Staphylococcus epidermidis is the most frequently detected coagulase-negative staphylococcus (CoNS) that is commonly responsible for catheter-related and central nervous system (CNS) infections. It is an opportunistic pathogen capable of forming biofilm which contributes to device-related infections and resistance to antibiotics. The ability to form biofilm has been linked with glycoproteins in biofilm-producing bacteria. This study investigates the differential glycoprotein expression between the biofilm producer and nonbiofilm producer of S. epidermidis and also its involvement in enhancing antibiotic resistance of the biofilm. S. epidermidis strains were subjected to N- and O-glycosylation inhibition followed by physical characterisation by Field Emission Electron Scanning Microscopy (FESEM) and antibiotic resistance profiling. The absence of O-glycosylation ascertains with reduced biofilm production of ATCC35984 strain and biofilm thickness. Subsequently, treatment with vancomycin, rifampicin and tetracycline showed that the antibiotics were more effective against O-glycan deficient biofilm. Differential protein expression was observed in planktonic and biofilm cultures of both biofilm and nonbiofilm producing strains. Glycoproteins were detected using Pro-Q® Emerald 300 Glycoprotein staining and subjected to mass spectrometry (LC-MS) identification. Out of the 17 spots characterized, 55 glycoproteins were identified for glycoprotein site prediction as well as protein interaction analysis using STRING tool and Clusters of Orthologous Genes (COGs). Majority of these proteins were found to carry either N- or O-glycosylation sites with some known to be related with biofilm formation such as glutamate synthase and influence antibiotic resistance for example glutamate-tRNA ligase. Therefore, O-glycans are suggested to have potential impact on biofilm formation and antibiotic resistance of S. epidermidis which warrants further evaluation and validation. 2021-11 Thesis NonPeerReviewed text en https://ir.uitm.edu.my/id/eprint/60522/1/60522.pdf (2021) Glycoprotein profiling of Staphylococcus epidermidis and its role in biofilm formation and antibiotic resistance / Nur Alia Othman. Masters thesis, thesis, Universiti Teknologi MARA.
institution Universiti Teknologi Mara
building Tun Abdul Razak Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Teknologi Mara
content_source UiTM Institutional Repository
url_provider http://ir.uitm.edu.my/
language English
topic Bacteria
spellingShingle Bacteria
Othman, Nur Alia
Glycoprotein profiling of Staphylococcus epidermidis and its role in biofilm formation and antibiotic resistance / Nur Alia Othman
description Staphylococcus epidermidis is the most frequently detected coagulase-negative staphylococcus (CoNS) that is commonly responsible for catheter-related and central nervous system (CNS) infections. It is an opportunistic pathogen capable of forming biofilm which contributes to device-related infections and resistance to antibiotics. The ability to form biofilm has been linked with glycoproteins in biofilm-producing bacteria. This study investigates the differential glycoprotein expression between the biofilm producer and nonbiofilm producer of S. epidermidis and also its involvement in enhancing antibiotic resistance of the biofilm. S. epidermidis strains were subjected to N- and O-glycosylation inhibition followed by physical characterisation by Field Emission Electron Scanning Microscopy (FESEM) and antibiotic resistance profiling. The absence of O-glycosylation ascertains with reduced biofilm production of ATCC35984 strain and biofilm thickness. Subsequently, treatment with vancomycin, rifampicin and tetracycline showed that the antibiotics were more effective against O-glycan deficient biofilm. Differential protein expression was observed in planktonic and biofilm cultures of both biofilm and nonbiofilm producing strains. Glycoproteins were detected using Pro-Q® Emerald 300 Glycoprotein staining and subjected to mass spectrometry (LC-MS) identification. Out of the 17 spots characterized, 55 glycoproteins were identified for glycoprotein site prediction as well as protein interaction analysis using STRING tool and Clusters of Orthologous Genes (COGs). Majority of these proteins were found to carry either N- or O-glycosylation sites with some known to be related with biofilm formation such as glutamate synthase and influence antibiotic resistance for example glutamate-tRNA ligase. Therefore, O-glycans are suggested to have potential impact on biofilm formation and antibiotic resistance of S. epidermidis which warrants further evaluation and validation.
format Thesis
author Othman, Nur Alia
author_facet Othman, Nur Alia
author_sort Othman, Nur Alia
title Glycoprotein profiling of Staphylococcus epidermidis and its role in biofilm formation and antibiotic resistance / Nur Alia Othman
title_short Glycoprotein profiling of Staphylococcus epidermidis and its role in biofilm formation and antibiotic resistance / Nur Alia Othman
title_full Glycoprotein profiling of Staphylococcus epidermidis and its role in biofilm formation and antibiotic resistance / Nur Alia Othman
title_fullStr Glycoprotein profiling of Staphylococcus epidermidis and its role in biofilm formation and antibiotic resistance / Nur Alia Othman
title_full_unstemmed Glycoprotein profiling of Staphylococcus epidermidis and its role in biofilm formation and antibiotic resistance / Nur Alia Othman
title_sort glycoprotein profiling of staphylococcus epidermidis and its role in biofilm formation and antibiotic resistance / nur alia othman
publishDate 2021
url https://ir.uitm.edu.my/id/eprint/60522/1/60522.pdf
https://ir.uitm.edu.my/id/eprint/60522/
_version_ 1734303101176774656
score 13.211869

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