Cover Image

Growth kinetic of rhizopus sp. immobilised on loofah sponge for whole-cell biocatalyst in different cultivation media / Azlin Suhaida Azmi … [et al.]

Immobilisation of filamentous fungi such as Rhizopus sp. onto biomass support particles (BSP) surface was studied as whole-cell biocatalyst. The function was to aid fermentation process. By using the microbial immobilisation process, the complex procedures of isolation, purification and immobilisati...

Full description

Saved in:
Bibliographic Details
Main Authors: Azmi, Azlin Suhaida, Mohd Bakari, Nur Atikah, Ali, Fathilah, Mohamad Puad, Noor Illi, Ahmad, Farah
Format: Article
Language:English
Published: Universiti Teknologi MARA 2022
Subjects:
TP Chemical technology
Online Access:https://ir.uitm.edu.my/id/eprint/60412/1/60412.pdf
https://ir.uitm.edu.my/id/eprint/60412/
https://myjms.mohe.gov.my
Tags: Add Tag
No Tags, Be the first to tag this record!
id my.uitm.ir.60412
record_format eprints
spelling my.uitm.ir.604122022-05-30T02:06:11Z https://ir.uitm.edu.my/id/eprint/60412/ Growth kinetic of rhizopus sp. immobilised on loofah sponge for whole-cell biocatalyst in different cultivation media / Azlin Suhaida Azmi … [et al.] Azmi, Azlin Suhaida Mohd Bakari, Nur Atikah Ali, Fathilah Mohamad Puad, Noor Illi Ahmad, Farah TP Chemical technology Immobilisation of filamentous fungi such as Rhizopus sp. onto biomass support particles (BSP) surface was studied as whole-cell biocatalyst. The function was to aid fermentation process. By using the microbial immobilisation process, the complex procedures of isolation, purification and immobilisation of extracellular enzyme can be avoided. Loofa sponge was selected as the BSP to aid in the immobilisation of the cells. In this study, the growth of immobilised Rhizopus sp. on loofa sponge was compared in four different cultivation media and the attachment of Rhizopus sp. on loofa sponge was investigated using scanning electron microscopy (SEM). The media used in this study were hydrolysed cassava starch, cassava dextrose, potato dextrose, and soy dextrose. The process condition and other parameters which were temperature, pH, inoculum dilution, and weight of loofa sponge, fixed at 30 °C, 7, 20 mL, and 2 g, respectively. The highest and lowest of maximum growth (ymax) of the immobilised cells were determined from potato dextrose and soy powder mixed with dextrose media, respectively at 1.5281 g/g and 1.0370 g/g. Whilst, the highest and lowest observed rate constant (k) were obtained from cassava starch mixed with dextrose and soy powder mixed with dextrose, which respectively at 2.9403 day−1 and −0.8763 day−1. SEM images showed the presence of mycelia attached to the loofah sponge after immobilisation process. In conclusion, Rhizopus sp. has been successfully immobilised on loofah sponge as a whole-cell biocatalyst. Universiti Teknologi MARA 2022-04 Article PeerReviewed text en https://ir.uitm.edu.my/id/eprint/60412/1/60412.pdf (2022) Growth kinetic of rhizopus sp. immobilised on loofah sponge for whole-cell biocatalyst in different cultivation media / Azlin Suhaida Azmi … [et al.]. Malaysian Journal of Chemical Engineering and Technology (MJCET), 5 (1): 5. pp. 38-42. ISSN (e-ISSN): 2682-8588 https://myjms.mohe.gov.my
institution Universiti Teknologi Mara
building Tun Abdul Razak Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Teknologi Mara
content_source UiTM Institutional Repository
url_provider http://ir.uitm.edu.my/
language English
topic TP Chemical technology
spellingShingle TP Chemical technology
Azmi, Azlin Suhaida
Mohd Bakari, Nur Atikah
Ali, Fathilah
Mohamad Puad, Noor Illi
Ahmad, Farah
Growth kinetic of rhizopus sp. immobilised on loofah sponge for whole-cell biocatalyst in different cultivation media / Azlin Suhaida Azmi … [et al.]
description Immobilisation of filamentous fungi such as Rhizopus sp. onto biomass support particles (BSP) surface was studied as whole-cell biocatalyst. The function was to aid fermentation process. By using the microbial immobilisation process, the complex procedures of isolation, purification and immobilisation of extracellular enzyme can be avoided. Loofa sponge was selected as the BSP to aid in the immobilisation of the cells. In this study, the growth of immobilised Rhizopus sp. on loofa sponge was compared in four different cultivation media and the attachment of Rhizopus sp. on loofa sponge was investigated using scanning electron microscopy (SEM). The media used in this study were hydrolysed cassava starch, cassava dextrose, potato dextrose, and soy dextrose. The process condition and other parameters which were temperature, pH, inoculum dilution, and weight of loofa sponge, fixed at 30 °C, 7, 20 mL, and 2 g, respectively. The highest and lowest of maximum growth (ymax) of the immobilised cells were determined from potato dextrose and soy powder mixed with dextrose media, respectively at 1.5281 g/g and 1.0370 g/g. Whilst, the highest and lowest observed rate constant (k) were obtained from cassava starch mixed with dextrose and soy powder mixed with dextrose, which respectively at 2.9403 day−1 and −0.8763 day−1. SEM images showed the presence of mycelia attached to the loofah sponge after immobilisation process. In conclusion, Rhizopus sp. has been successfully immobilised on loofah sponge as a whole-cell biocatalyst.
format Article
author Azmi, Azlin Suhaida
Mohd Bakari, Nur Atikah
Ali, Fathilah
Mohamad Puad, Noor Illi
Ahmad, Farah
author_facet Azmi, Azlin Suhaida
Mohd Bakari, Nur Atikah
Ali, Fathilah
Mohamad Puad, Noor Illi
Ahmad, Farah
author_sort Azmi, Azlin Suhaida
title Growth kinetic of rhizopus sp. immobilised on loofah sponge for whole-cell biocatalyst in different cultivation media / Azlin Suhaida Azmi … [et al.]
title_short Growth kinetic of rhizopus sp. immobilised on loofah sponge for whole-cell biocatalyst in different cultivation media / Azlin Suhaida Azmi … [et al.]
title_full Growth kinetic of rhizopus sp. immobilised on loofah sponge for whole-cell biocatalyst in different cultivation media / Azlin Suhaida Azmi … [et al.]
title_fullStr Growth kinetic of rhizopus sp. immobilised on loofah sponge for whole-cell biocatalyst in different cultivation media / Azlin Suhaida Azmi … [et al.]
title_full_unstemmed Growth kinetic of rhizopus sp. immobilised on loofah sponge for whole-cell biocatalyst in different cultivation media / Azlin Suhaida Azmi … [et al.]
title_sort growth kinetic of rhizopus sp. immobilised on loofah sponge for whole-cell biocatalyst in different cultivation media / azlin suhaida azmi … [et al.]
publisher Universiti Teknologi MARA
publishDate 2022
url https://ir.uitm.edu.my/id/eprint/60412/1/60412.pdf
https://ir.uitm.edu.my/id/eprint/60412/
https://myjms.mohe.gov.my
_version_ 1734303094125101056
score 13.211869

Similar Items