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Effects of different culturing systems on micropropagation melastoma decemfidum / Nurul Athirah Mohamad Adam

Melastoma decemfidum is an important medicinal plant as it contains the anticancer compounds kaempferol and naringenin. As traditional propagation via stem cutting has its limitations and is time consuming, micropropagation by tissue culture technique can be considered as an alternative method to cu...

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Main Author: Mohamad Adam, Nurul Athirah
Format: Thesis
Language:English
Published: 2021
Subjects:
Clinical pathology. Laboratory technique
Online Access:https://ir.uitm.edu.my/id/eprint/59714/1/59714.pdf
https://ir.uitm.edu.my/id/eprint/59714/
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spelling my.uitm.ir.597142022-05-17T05:32:24Z https://ir.uitm.edu.my/id/eprint/59714/ Effects of different culturing systems on micropropagation melastoma decemfidum / Nurul Athirah Mohamad Adam Mohamad Adam, Nurul Athirah Clinical pathology. Laboratory technique Melastoma decemfidum is an important medicinal plant as it contains the anticancer compounds kaempferol and naringenin. As traditional propagation via stem cutting has its limitations and is time consuming, micropropagation by tissue culture technique can be considered as an alternative method to cultivate the plant. This research aims to optimize in vitro micropropagation of M.decemfidum for its rapid mass propagation and continuous supply. In this study, an efficient protocol that utilised BAP and NAA individually and in combination was studied. About 1 cm of nodal explants of in vitro plantlets of M. decemfidum were cultured onto 25 treatments MS media supplemented with various combinations of BAP and NAA hormones (0.1, 0.2, 0.25, 0.3 mg/L). The highest mean number of shoots at 9.67 ± 0.33 and shoot length at 0.61 ± 0.03 cm were obtained from the nodal explant cultured on 0.30 mg/L BAP. The highest number of leaves at 23.83 ± 0.47 and roots at 4.33 ± 0.33 were recorded for the nodal explant in the MS media containing 0.25 mg/L BAP and 0.1 mg/L BAP in combination with 0.2 mg/L NAA. The experiment also revealed that the combination of BAP and NAA hormones encourages callus formation (indirect regeneration). Then, the best treatment was used to study the effects of different culture systems on M. decemfidum’s growth rate. The second research involved the micropropagation of in vitro plantlets of M. decemfidum via different culture systems such as agar gelled cultures (AGCS), permanent immersion culture system (PICS) and temporary immersion bioreactor culture system (TIBS). Under TIBS, in vitro plantlets were temporary immersed in liquid nutrient medium. The use of TIBS showed many quantitative benefits, most notably recording the highest proliferation rate in comparison with both solid and liquid culture systems. As mentioned, in vitro plantlets cultured in temporary immersion bioreactor (TIBS) recorded the highest growth rate with significance differences (p<0.05) in terms of shoot multiplication (4.62 ± 0.39), shoot length (0.34 ± 0.03) cm and leaf number (10.67 ± 0.54) compared to PICS and AGCS. Therefore, it can be said that in this study, the in vitro propagation of M. decemfidum was successfully optimized using TIBS. Considering the importance of detection of secondary metabolites in medicinal plants, Total Phenolic Content (TPC) was evaluated at different growth conditions. From the results obtained, the highest TPC reading of 25.32 ± 1.06 mg/g was for the TPC of ex vitro leaves, followed by in vivo (23.00 ± 1.60 mg/g), in vitro leaves cultured from TIBS (9.70 ± 0.34 mg/g), in vitro leaves from PICS (8.24 ± 0.34 mg/g) and in vitro leaves from AGCS (7.46 ± 0.24 mg/g). ANOVA analysis conducted showed that there is significance difference (p<0.05) between the TPC results of five samples. The highest TPC result showed that the ex vitro of M. decemfidum obtained using the acclimatization from plant tissue culture technique contains high contents of phenolic compounds which can be considered a good source of secondary metabolites. 2021-03 Thesis NonPeerReviewed text en https://ir.uitm.edu.my/id/eprint/59714/1/59714.pdf (2021) Effects of different culturing systems on micropropagation melastoma decemfidum / Nurul Athirah Mohamad Adam. Masters thesis, thesis, Universiti Teknologi MARA.
institution Universiti Teknologi Mara
building Tun Abdul Razak Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Teknologi Mara
content_source UiTM Institutional Repository
url_provider http://ir.uitm.edu.my/
language English
topic Clinical pathology. Laboratory technique
spellingShingle Clinical pathology. Laboratory technique
Mohamad Adam, Nurul Athirah
Effects of different culturing systems on micropropagation melastoma decemfidum / Nurul Athirah Mohamad Adam
description Melastoma decemfidum is an important medicinal plant as it contains the anticancer compounds kaempferol and naringenin. As traditional propagation via stem cutting has its limitations and is time consuming, micropropagation by tissue culture technique can be considered as an alternative method to cultivate the plant. This research aims to optimize in vitro micropropagation of M.decemfidum for its rapid mass propagation and continuous supply. In this study, an efficient protocol that utilised BAP and NAA individually and in combination was studied. About 1 cm of nodal explants of in vitro plantlets of M. decemfidum were cultured onto 25 treatments MS media supplemented with various combinations of BAP and NAA hormones (0.1, 0.2, 0.25, 0.3 mg/L). The highest mean number of shoots at 9.67 ± 0.33 and shoot length at 0.61 ± 0.03 cm were obtained from the nodal explant cultured on 0.30 mg/L BAP. The highest number of leaves at 23.83 ± 0.47 and roots at 4.33 ± 0.33 were recorded for the nodal explant in the MS media containing 0.25 mg/L BAP and 0.1 mg/L BAP in combination with 0.2 mg/L NAA. The experiment also revealed that the combination of BAP and NAA hormones encourages callus formation (indirect regeneration). Then, the best treatment was used to study the effects of different culture systems on M. decemfidum’s growth rate. The second research involved the micropropagation of in vitro plantlets of M. decemfidum via different culture systems such as agar gelled cultures (AGCS), permanent immersion culture system (PICS) and temporary immersion bioreactor culture system (TIBS). Under TIBS, in vitro plantlets were temporary immersed in liquid nutrient medium. The use of TIBS showed many quantitative benefits, most notably recording the highest proliferation rate in comparison with both solid and liquid culture systems. As mentioned, in vitro plantlets cultured in temporary immersion bioreactor (TIBS) recorded the highest growth rate with significance differences (p<0.05) in terms of shoot multiplication (4.62 ± 0.39), shoot length (0.34 ± 0.03) cm and leaf number (10.67 ± 0.54) compared to PICS and AGCS. Therefore, it can be said that in this study, the in vitro propagation of M. decemfidum was successfully optimized using TIBS. Considering the importance of detection of secondary metabolites in medicinal plants, Total Phenolic Content (TPC) was evaluated at different growth conditions. From the results obtained, the highest TPC reading of 25.32 ± 1.06 mg/g was for the TPC of ex vitro leaves, followed by in vivo (23.00 ± 1.60 mg/g), in vitro leaves cultured from TIBS (9.70 ± 0.34 mg/g), in vitro leaves from PICS (8.24 ± 0.34 mg/g) and in vitro leaves from AGCS (7.46 ± 0.24 mg/g). ANOVA analysis conducted showed that there is significance difference (p<0.05) between the TPC results of five samples. The highest TPC result showed that the ex vitro of M. decemfidum obtained using the acclimatization from plant tissue culture technique contains high contents of phenolic compounds which can be considered a good source of secondary metabolites.
format Thesis
author Mohamad Adam, Nurul Athirah
author_facet Mohamad Adam, Nurul Athirah
author_sort Mohamad Adam, Nurul Athirah
title Effects of different culturing systems on micropropagation melastoma decemfidum / Nurul Athirah Mohamad Adam
title_short Effects of different culturing systems on micropropagation melastoma decemfidum / Nurul Athirah Mohamad Adam
title_full Effects of different culturing systems on micropropagation melastoma decemfidum / Nurul Athirah Mohamad Adam
title_fullStr Effects of different culturing systems on micropropagation melastoma decemfidum / Nurul Athirah Mohamad Adam
title_full_unstemmed Effects of different culturing systems on micropropagation melastoma decemfidum / Nurul Athirah Mohamad Adam
title_sort effects of different culturing systems on micropropagation melastoma decemfidum / nurul athirah mohamad adam
publishDate 2021
url https://ir.uitm.edu.my/id/eprint/59714/1/59714.pdf
https://ir.uitm.edu.my/id/eprint/59714/
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score 13.211869

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