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Isolation and screening antagonistic activity Endophytic bacteria from oil palm roots against Ganoderma spp. in different age of oil palm in vitro / Azerul Kamahudin and Mohd Zafri Ab Wahab

The oil palm (Elaeis guineensis) is commodity crop for Malaysia. In this study, an experiment was conducted to screening antagonistic activity of endophytic bacteria from oil palm roots. The aims was to identify bacteria that can be used to control Ganoderma sp. Root sample was taken from three diff...

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Main Authors: Kamahudin, Azerul, Ab Wahab, Mohd Zafri
Format: Book Section
Language:English
Published: Faculty of Plantation and Agrotechnology 2018
Subjects:
Bacteria
S Agriculture (General)
SB Plant culture
Online Access:https://ir.uitm.edu.my/id/eprint/59169/1/59169.pdf
https://ir.uitm.edu.my/id/eprint/59169/
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spelling my.uitm.ir.591692022-06-29T08:39:37Z https://ir.uitm.edu.my/id/eprint/59169/ Isolation and screening antagonistic activity Endophytic bacteria from oil palm roots against Ganoderma spp. in different age of oil palm in vitro / Azerul Kamahudin and Mohd Zafri Ab Wahab Kamahudin, Azerul Ab Wahab, Mohd Zafri Bacteria S Agriculture (General) SB Plant culture The oil palm (Elaeis guineensis) is commodity crop for Malaysia. In this study, an experiment was conducted to screening antagonistic activity of endophytic bacteria from oil palm roots. The aims was to identify bacteria that can be used to control Ganoderma sp. Root sample was taken from three different place that is Segamat estate Johor, nursery Fong Shun trading Rompin and ladang uitm jasin, Melaka. For isolation of Ganoderma sp sample was being taken at private company research center at Gemencheh Melaka. A total of 30 roots sample were obtained with 10 sample from segamat estate Johor, 10 sample roots for in mature from nursery Fong Shun trading and 10 root samples form mature oil palm from ladang uitm Jasin. Bacteria that being isolates showed the antagonistic activity against Ganoderma sp by inhibition and slow down the growth rate of Ganoderma sp in culture plate. 27 endophytic bacteria were successfully isolated from roots sample and about 9 of it show the positive result, that slow down the growth of Ganoderma sp. Six out of 9 isolate were identified as Gram-negative bacteria and 3 were Gram-positive bacteria. Therefore, Gram-negative bacteria more in the oil palm roots compared to the Gram-positive bacteria. From 9 bacteria, only 2 bacteria that is success to suppress Ganoderma sp more the 50 % using the RIPG calculation method. These bacteria were identified and characterized. The bacteria colony form or shape is round and rod shape. Colony margin is entire and filamentous. Three type Colony elevation was identify that is raised, convex, and growth into medium. Faculty of Plantation and Agrotechnology 2018-12 Book Section PeerReviewed text en https://ir.uitm.edu.my/id/eprint/59169/1/59169.pdf Isolation and screening antagonistic activity Endophytic bacteria from oil palm roots against Ganoderma spp. in different age of oil palm in vitro / Azerul Kamahudin and Mohd Zafri Ab Wahab. (2018) In: Plantation Management Exhibition & Seminar (PIMES). Faculty of Plantation and Agrotechnology, Kampus Jasin, p. 209.
institution Universiti Teknologi Mara
building Tun Abdul Razak Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Teknologi Mara
content_source UiTM Institutional Repository
url_provider http://ir.uitm.edu.my/
language English
topic Bacteria
S Agriculture (General)
SB Plant culture
spellingShingle Bacteria
S Agriculture (General)
SB Plant culture
Kamahudin, Azerul
Ab Wahab, Mohd Zafri
Isolation and screening antagonistic activity Endophytic bacteria from oil palm roots against Ganoderma spp. in different age of oil palm in vitro / Azerul Kamahudin and Mohd Zafri Ab Wahab
description The oil palm (Elaeis guineensis) is commodity crop for Malaysia. In this study, an experiment was conducted to screening antagonistic activity of endophytic bacteria from oil palm roots. The aims was to identify bacteria that can be used to control Ganoderma sp. Root sample was taken from three different place that is Segamat estate Johor, nursery Fong Shun trading Rompin and ladang uitm jasin, Melaka. For isolation of Ganoderma sp sample was being taken at private company research center at Gemencheh Melaka. A total of 30 roots sample were obtained with 10 sample from segamat estate Johor, 10 sample roots for in mature from nursery Fong Shun trading and 10 root samples form mature oil palm from ladang uitm Jasin. Bacteria that being isolates showed the antagonistic activity against Ganoderma sp by inhibition and slow down the growth rate of Ganoderma sp in culture plate. 27 endophytic bacteria were successfully isolated from roots sample and about 9 of it show the positive result, that slow down the growth of Ganoderma sp. Six out of 9 isolate were identified as Gram-negative bacteria and 3 were Gram-positive bacteria. Therefore, Gram-negative bacteria more in the oil palm roots compared to the Gram-positive bacteria. From 9 bacteria, only 2 bacteria that is success to suppress Ganoderma sp more the 50 % using the RIPG calculation method. These bacteria were identified and characterized. The bacteria colony form or shape is round and rod shape. Colony margin is entire and filamentous. Three type Colony elevation was identify that is raised, convex, and growth into medium.
format Book Section
author Kamahudin, Azerul
Ab Wahab, Mohd Zafri
author_facet Kamahudin, Azerul
Ab Wahab, Mohd Zafri
author_sort Kamahudin, Azerul
title Isolation and screening antagonistic activity Endophytic bacteria from oil palm roots against Ganoderma spp. in different age of oil palm in vitro / Azerul Kamahudin and Mohd Zafri Ab Wahab
title_short Isolation and screening antagonistic activity Endophytic bacteria from oil palm roots against Ganoderma spp. in different age of oil palm in vitro / Azerul Kamahudin and Mohd Zafri Ab Wahab
title_full Isolation and screening antagonistic activity Endophytic bacteria from oil palm roots against Ganoderma spp. in different age of oil palm in vitro / Azerul Kamahudin and Mohd Zafri Ab Wahab
title_fullStr Isolation and screening antagonistic activity Endophytic bacteria from oil palm roots against Ganoderma spp. in different age of oil palm in vitro / Azerul Kamahudin and Mohd Zafri Ab Wahab
title_full_unstemmed Isolation and screening antagonistic activity Endophytic bacteria from oil palm roots against Ganoderma spp. in different age of oil palm in vitro / Azerul Kamahudin and Mohd Zafri Ab Wahab
title_sort isolation and screening antagonistic activity endophytic bacteria from oil palm roots against ganoderma spp. in different age of oil palm in vitro / azerul kamahudin and mohd zafri ab wahab
publisher Faculty of Plantation and Agrotechnology
publishDate 2018
url https://ir.uitm.edu.my/id/eprint/59169/1/59169.pdf
https://ir.uitm.edu.my/id/eprint/59169/
_version_ 1738513938700566528
score 13.211869

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