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The efficiency of long primers compared to the short primer for RAPD technique in date palm / Aisyah Mohd Ismail and Farida Zuraina Mohd

Random Amplified Polymorphic DNA (RAPD) applies single arbitrary short primers (8-12 nucleotides) to produce many amplified discrete DNA. Limited reports and studies were done on the use of long primers (over 12 bases). This study was performed to investigate the potential value of long primers (15-...

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Main Authors: Mohd Ismail, Aisyah, Mohd, Farida Zuraina
Format: Article
Language:English
Published: Universiti Teknologi MARA 2022
Subjects:
Chemical engineering
Biotechnology
Online Access:https://ir.uitm.edu.my/id/eprint/58230/1/58230.pdf
https://ir.uitm.edu.my/id/eprint/58230/
https://scilett-fsg.uitm.edu.my/
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spelling my.uitm.ir.582302022-04-13T03:12:35Z https://ir.uitm.edu.my/id/eprint/58230/ The efficiency of long primers compared to the short primer for RAPD technique in date palm / Aisyah Mohd Ismail and Farida Zuraina Mohd Mohd Ismail, Aisyah Mohd, Farida Zuraina Chemical engineering Biotechnology Random Amplified Polymorphic DNA (RAPD) applies single arbitrary short primers (8-12 nucleotides) to produce many amplified discrete DNA. Limited reports and studies were done on the use of long primers (over 12 bases). This study was performed to investigate the potential value of long primers (15-21 bases) for generating RAPD polymorphisms. We compared both short and long primers in RAPD assays of two date palm cultivars grown in Malaysia: Ajwa and Barhi. The number of produced polymorphic fragments ranged in order from 2 and 38 bands for short and long primers in Ajwa. Meanwhile, more polymorphic fragments were generated by long primers in Barhi, which were 50 and only five bands for short primers. 18-mer GY107 and 20-mer CO4 primers yielded 100% polymorphism in Ajwa and Barhi, respectively. Moreover, long primers produced more DNA fragments and a wider range of DNA fragment sizes (from 140-1600 bp, with respect to 300-1000 bp obtained with 10-mer primers). Hence, a significant correlation was observed between primer length and the number of polymorphic fragments within the long primer group, suggesting that increasing primer length above 15 bases may demonstrate enhanced production of more polymorphism. Universiti Teknologi MARA 2022-01 Article PeerReviewed text en https://ir.uitm.edu.my/id/eprint/58230/1/58230.pdf (2022) The efficiency of long primers compared to the short primer for RAPD technique in date palm / Aisyah Mohd Ismail and Farida Zuraina Mohd. Science Letters (ScL), 16 (1): 11. pp. 1-11. ISSN (eISSN): 2682-8626 https://scilett-fsg.uitm.edu.my/
institution Universiti Teknologi Mara
building Tun Abdul Razak Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Teknologi Mara
content_source UiTM Institutional Repository
url_provider http://ir.uitm.edu.my/
language English
topic Chemical engineering
Biotechnology
spellingShingle Chemical engineering
Biotechnology
Mohd Ismail, Aisyah
Mohd, Farida Zuraina
The efficiency of long primers compared to the short primer for RAPD technique in date palm / Aisyah Mohd Ismail and Farida Zuraina Mohd
description Random Amplified Polymorphic DNA (RAPD) applies single arbitrary short primers (8-12 nucleotides) to produce many amplified discrete DNA. Limited reports and studies were done on the use of long primers (over 12 bases). This study was performed to investigate the potential value of long primers (15-21 bases) for generating RAPD polymorphisms. We compared both short and long primers in RAPD assays of two date palm cultivars grown in Malaysia: Ajwa and Barhi. The number of produced polymorphic fragments ranged in order from 2 and 38 bands for short and long primers in Ajwa. Meanwhile, more polymorphic fragments were generated by long primers in Barhi, which were 50 and only five bands for short primers. 18-mer GY107 and 20-mer CO4 primers yielded 100% polymorphism in Ajwa and Barhi, respectively. Moreover, long primers produced more DNA fragments and a wider range of DNA fragment sizes (from 140-1600 bp, with respect to 300-1000 bp obtained with 10-mer primers). Hence, a significant correlation was observed between primer length and the number of polymorphic fragments within the long primer group, suggesting that increasing primer length above 15 bases may demonstrate enhanced production of more polymorphism.
format Article
author Mohd Ismail, Aisyah
Mohd, Farida Zuraina
author_facet Mohd Ismail, Aisyah
Mohd, Farida Zuraina
author_sort Mohd Ismail, Aisyah
title The efficiency of long primers compared to the short primer for RAPD technique in date palm / Aisyah Mohd Ismail and Farida Zuraina Mohd
title_short The efficiency of long primers compared to the short primer for RAPD technique in date palm / Aisyah Mohd Ismail and Farida Zuraina Mohd
title_full The efficiency of long primers compared to the short primer for RAPD technique in date palm / Aisyah Mohd Ismail and Farida Zuraina Mohd
title_fullStr The efficiency of long primers compared to the short primer for RAPD technique in date palm / Aisyah Mohd Ismail and Farida Zuraina Mohd
title_full_unstemmed The efficiency of long primers compared to the short primer for RAPD technique in date palm / Aisyah Mohd Ismail and Farida Zuraina Mohd
title_sort efficiency of long primers compared to the short primer for rapd technique in date palm / aisyah mohd ismail and farida zuraina mohd
publisher Universiti Teknologi MARA
publishDate 2022
url https://ir.uitm.edu.my/id/eprint/58230/1/58230.pdf
https://ir.uitm.edu.my/id/eprint/58230/
https://scilett-fsg.uitm.edu.my/
_version_ 1731229115351040000
score 13.211869

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