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Method validation and determination of saccharin in sport drinks by spectrophotometric technique / Muhammad Faaiez Yunus

Saccharin is one of the most well-known artificial sweetener that widely used since a long time ago. Saccharin is commonly used as sweetener to replace sugar in foods and beverages as it is 300-400 times sweeter than normal sugar. The presence of saccharin that exceeds the permitted level of 300 mg...

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Main Author: Yunus, Muhammad Faaiez
Format: Student Project
Language:English
Published: 2020
Subjects:
Sports
Nutrition. Foods and food supply
Beverages
Online Access:https://ir.uitm.edu.my/id/eprint/55772/1/55772.pdf
https://ir.uitm.edu.my/id/eprint/55772/
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spelling my.uitm.ir.557722022-12-01T08:13:01Z https://ir.uitm.edu.my/id/eprint/55772/ Method validation and determination of saccharin in sport drinks by spectrophotometric technique / Muhammad Faaiez Yunus Yunus, Muhammad Faaiez Sports Nutrition. Foods and food supply Beverages Saccharin is one of the most well-known artificial sweetener that widely used since a long time ago. Saccharin is commonly used as sweetener to replace sugar in foods and beverages as it is 300-400 times sweeter than normal sugar. The presence of saccharin that exceeds the permitted level of 300 mg L-1 in drinks might cause severe health effect to the consumers. Hence, the amount of saccharin in drink products must be analyzed. A sensitive, accurate, simple, rapid and low-cost analytical method is required for the determination of saccharin. The spectrophotometric method has been proposed for the quantitative analysis of saccharin. The calibration curve was linear from 0 mg L-1 to 10 mg L-1 of saccharin standard solution with a regression coefficient (R2) of 0.9999. The limit of detection (LOD) obtained was 0.25 mg L-1. The precisions in terms of relative standard deviation (RSD) were 0.39 %, 0.28 % and 0.69 % for 2 mg L-1 saccharin in consecutive three days. Meanwhile, the RSD were 0.62 %, 0.23 % and 0.06 % for 5 mg L-1. Lastly for concentration of 8 mg L-1, the RSD were 0.11 %, 0.07 % and 0.07 % for three consecutive days. The recoveries achieved for 3 and 6 mg L-1 of saccharin standard solution in the isotonic sport drinks were 89.33 % and 92.66 %, respectively. Three tested isotonic sport drinks contain no saccharin and the rest contain saccharin below the permitted level. It can be concluded that this proposed method is accurate, simple, fast, low cost and has a potential to be an alternative method for routine analysis of saccharin in isotonic sport drinks. 2020 Student Project NonPeerReviewed text en https://ir.uitm.edu.my/id/eprint/55772/1/55772.pdf Method validation and determination of saccharin in sport drinks by spectrophotometric technique / Muhammad Faaiez Yunus. (2020) [Student Project] <http://terminalib.uitm.edu.my/55772.pdf> (Unpublished)
institution Universiti Teknologi Mara
building Tun Abdul Razak Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Teknologi Mara
content_source UiTM Institutional Repository
url_provider http://ir.uitm.edu.my/
language English
topic Sports
Nutrition. Foods and food supply
Beverages
spellingShingle Sports
Nutrition. Foods and food supply
Beverages
Yunus, Muhammad Faaiez
Method validation and determination of saccharin in sport drinks by spectrophotometric technique / Muhammad Faaiez Yunus
description Saccharin is one of the most well-known artificial sweetener that widely used since a long time ago. Saccharin is commonly used as sweetener to replace sugar in foods and beverages as it is 300-400 times sweeter than normal sugar. The presence of saccharin that exceeds the permitted level of 300 mg L-1 in drinks might cause severe health effect to the consumers. Hence, the amount of saccharin in drink products must be analyzed. A sensitive, accurate, simple, rapid and low-cost analytical method is required for the determination of saccharin. The spectrophotometric method has been proposed for the quantitative analysis of saccharin. The calibration curve was linear from 0 mg L-1 to 10 mg L-1 of saccharin standard solution with a regression coefficient (R2) of 0.9999. The limit of detection (LOD) obtained was 0.25 mg L-1. The precisions in terms of relative standard deviation (RSD) were 0.39 %, 0.28 % and 0.69 % for 2 mg L-1 saccharin in consecutive three days. Meanwhile, the RSD were 0.62 %, 0.23 % and 0.06 % for 5 mg L-1. Lastly for concentration of 8 mg L-1, the RSD were 0.11 %, 0.07 % and 0.07 % for three consecutive days. The recoveries achieved for 3 and 6 mg L-1 of saccharin standard solution in the isotonic sport drinks were 89.33 % and 92.66 %, respectively. Three tested isotonic sport drinks contain no saccharin and the rest contain saccharin below the permitted level. It can be concluded that this proposed method is accurate, simple, fast, low cost and has a potential to be an alternative method for routine analysis of saccharin in isotonic sport drinks.
format Student Project
author Yunus, Muhammad Faaiez
author_facet Yunus, Muhammad Faaiez
author_sort Yunus, Muhammad Faaiez
title Method validation and determination of saccharin in sport drinks by spectrophotometric technique / Muhammad Faaiez Yunus
title_short Method validation and determination of saccharin in sport drinks by spectrophotometric technique / Muhammad Faaiez Yunus
title_full Method validation and determination of saccharin in sport drinks by spectrophotometric technique / Muhammad Faaiez Yunus
title_fullStr Method validation and determination of saccharin in sport drinks by spectrophotometric technique / Muhammad Faaiez Yunus
title_full_unstemmed Method validation and determination of saccharin in sport drinks by spectrophotometric technique / Muhammad Faaiez Yunus
title_sort method validation and determination of saccharin in sport drinks by spectrophotometric technique / muhammad faaiez yunus
publishDate 2020
url https://ir.uitm.edu.my/id/eprint/55772/1/55772.pdf
https://ir.uitm.edu.my/id/eprint/55772/
_version_ 1751539842158690304
score 13.211869

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