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Optimization of hydrolysis conditions for the production of shrimp hydrolysate by using protamex: effect of enzyme substrate (ES) ratio and time / Nurul Nadiah Ahmad Latif.

The optimization of hydrolysis conditions which are enzyme substrate ratio ..and hydrolysis time for the production of shrimp hydrolysate by using protamex were studied. This study was done to obtain the optimum percentage for nitrogen recovery and degree of hydrolysis on the hydrolysis conditions....

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Main Author:	Ahmad Latif, Nurul Nadiah
Format:	Thesis
Language:	English
Published:	2009
Subjects:	Chemical engineering Hydrolysis Biotechnology
Online Access:	https://ir.uitm.edu.my/id/eprint/53000/1/53000.pdf https://ir.uitm.edu.my/id/eprint/53000/
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spelling	my.uitm.ir.530002021-12-23T04:48:56Z https://ir.uitm.edu.my/id/eprint/53000/ Optimization of hydrolysis conditions for the production of shrimp hydrolysate by using protamex: effect of enzyme substrate (ES) ratio and time / Nurul Nadiah Ahmad Latif. Ahmad Latif, Nurul Nadiah Chemical engineering Hydrolysis Biotechnology The optimization of hydrolysis conditions which are enzyme substrate ratio ..and hydrolysis time for the production of shrimp hydrolysate by using protamex were studied. This study was done to obtain the optimum percentage for nitrogen recovery and degree of hydrolysis on the hydrolysis conditions. Methods for production of shrimp hydrolysate include homogenization, adjustment of pH and temperature, addition of enzyme and hydrolysis time which are based on response surface methodology (RSM) suggestion, termination of hydrolysis, cooled and centrifuged the hydrolysis sample, collection of supernatant and analysis for percentage of nitrogen recovery and degree of hydrolysis. Total nitrogen in supernatant was determined by using Kjeldhal method. The percentage of degree of hydrolysis was determined by using modified formol titration method. The data that were obtained from the experiment were accomplished by employing the RSM using Design Expert 4 Software. By fitting the experimental data to the equation, the percentage nitrogen recovery (%NR) optimum levels for time (240 min), ES ratio (0%), (%NR) (3.90308) with desirability (0.657) at constant pH 6 and constant temperature 60°C were determined. For percentage degree of hydrolysis (%DH), the optimum levels for time (0 min), ES ratio (4%), %DH (2.80186) with desirability (0.693) were determined. 2009 Thesis NonPeerReviewed text en https://ir.uitm.edu.my/id/eprint/53000/1/53000.pdf ID53000 Ahmad Latif, Nurul Nadiah (2009) Optimization of hydrolysis conditions for the production of shrimp hydrolysate by using protamex: effect of enzyme substrate (ES) ratio and time / Nurul Nadiah Ahmad Latif. Degree thesis, thesis, Universiti Teknologi MARA.
institution	Universiti Teknologi Mara
building	Tun Abdul Razak Library
collection	Institutional Repository
continent	Asia
country	Malaysia
content_provider	Universiti Teknologi Mara
content_source	UiTM Institutional Repository
url_provider	http://ir.uitm.edu.my/
language	English
topic	Chemical engineering Hydrolysis Biotechnology
spellingShingle	Chemical engineering Hydrolysis Biotechnology Ahmad Latif, Nurul Nadiah Optimization of hydrolysis conditions for the production of shrimp hydrolysate by using protamex: effect of enzyme substrate (ES) ratio and time / Nurul Nadiah Ahmad Latif.
description	The optimization of hydrolysis conditions which are enzyme substrate ratio ..and hydrolysis time for the production of shrimp hydrolysate by using protamex were studied. This study was done to obtain the optimum percentage for nitrogen recovery and degree of hydrolysis on the hydrolysis conditions. Methods for production of shrimp hydrolysate include homogenization, adjustment of pH and temperature, addition of enzyme and hydrolysis time which are based on response surface methodology (RSM) suggestion, termination of hydrolysis, cooled and centrifuged the hydrolysis sample, collection of supernatant and analysis for percentage of nitrogen recovery and degree of hydrolysis. Total nitrogen in supernatant was determined by using Kjeldhal method. The percentage of degree of hydrolysis was determined by using modified formol titration method. The data that were obtained from the experiment were accomplished by employing the RSM using Design Expert 4 Software. By fitting the experimental data to the equation, the percentage nitrogen recovery (%NR) optimum levels for time (240 min), ES ratio (0%), (%NR) (3.90308) with desirability (0.657) at constant pH 6 and constant temperature 60°C were determined. For percentage degree of hydrolysis (%DH), the optimum levels for time (0 min), ES ratio (4%), %DH (2.80186) with desirability (0.693) were determined.
format	Thesis
author	Ahmad Latif, Nurul Nadiah
author_facet	Ahmad Latif, Nurul Nadiah
author_sort	Ahmad Latif, Nurul Nadiah
title	Optimization of hydrolysis conditions for the production of shrimp hydrolysate by using protamex: effect of enzyme substrate (ES) ratio and time / Nurul Nadiah Ahmad Latif.
title_short	Optimization of hydrolysis conditions for the production of shrimp hydrolysate by using protamex: effect of enzyme substrate (ES) ratio and time / Nurul Nadiah Ahmad Latif.
title_full	Optimization of hydrolysis conditions for the production of shrimp hydrolysate by using protamex: effect of enzyme substrate (ES) ratio and time / Nurul Nadiah Ahmad Latif.
title_fullStr	Optimization of hydrolysis conditions for the production of shrimp hydrolysate by using protamex: effect of enzyme substrate (ES) ratio and time / Nurul Nadiah Ahmad Latif.
title_full_unstemmed	Optimization of hydrolysis conditions for the production of shrimp hydrolysate by using protamex: effect of enzyme substrate (ES) ratio and time / Nurul Nadiah Ahmad Latif.
title_sort	optimization of hydrolysis conditions for the production of shrimp hydrolysate by using protamex: effect of enzyme substrate (es) ratio and time / nurul nadiah ahmad latif.
publishDate	2009
url	https://ir.uitm.edu.my/id/eprint/53000/1/53000.pdf https://ir.uitm.edu.my/id/eprint/53000/
_version_	1720983243460706304
score	13.211869

Optimization of hydrolysis conditions for the production of shrimp hydrolysate by using protamex: effect of enzyme substrate (ES) ratio and time / Nurul Nadiah Ahmad Latif.

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