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The use of molecular tool in identifying potentially probiotic lactobacillus spp. isolated from cow's and goat's milk / Noor 'Iffah Hanani Mohd Ibrahim

The interest in consuming products which contained probiotic especially Lactobacillus spp. are at increasing trend since they have been proved to offer numbers of health benefit on human. At present, Lactobacillus spp. are known to exist in many different sources but studies on those sources remain...

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Main Author: Mohd Ibrahim, Noor 'Iffah Hanani
Format: Thesis
Language:English
Published: 2019
Subjects:
Immunology
Bacteria
Personal health and hygiene. Including clothing, bathing, exercise, travel, nutrition, sleep, sex hygiene
Cows
Online Access:https://ir.uitm.edu.my/id/eprint/50948/1/50948.pdf
https://ir.uitm.edu.my/id/eprint/50948/
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spelling my.uitm.ir.509482021-09-28T04:04:41Z https://ir.uitm.edu.my/id/eprint/50948/ The use of molecular tool in identifying potentially probiotic lactobacillus spp. isolated from cow's and goat's milk / Noor 'Iffah Hanani Mohd Ibrahim Mohd Ibrahim, Noor 'Iffah Hanani Immunology Bacteria Personal health and hygiene. Including clothing, bathing, exercise, travel, nutrition, sleep, sex hygiene Cows The interest in consuming products which contained probiotic especially Lactobacillus spp. are at increasing trend since they have been proved to offer numbers of health benefit on human. At present, Lactobacillus spp. are known to exist in many different sources but studies on those sources remain limited in Malaysia. Hence, investigation on two sources of potentially probiotic lactobacilli which are cow’s and goat’s milk obtained from local farms in Malaysia was conducted. This research focused on isolating and identifying potentially probiotic Lactobacillus spp. from cow’s and goat’s milk using both phenotypic and genotypic characterization. Initially, isolates from both samples were selected by phenotypic identification and further identified by molecular technique. DNA of isolates were extracted using NucleoSpin® Microbial DNA kit. Next, PCR amplification of 16S rRNA gene was performed using forward primer, 5' - GCT GGA TCA CCT CCT TTC - 3' and reverse primer, 5' - CCT TTC CCT CAC GGT ACT G - 3'. Amplicons were detected through 1.2 % agarose gel electrophoresis and visualized using ImageQuantᵀᴹ LAS 500. Bile salt tolerance was then performed to screen for probiotic property of six chosen isolates and their safety profiling was conducted through antibiotic susceptibility testing (AST). A total of 11 isolates revealed to be Gram-positive rod, negative for catalase and oxidase test were subjected to molecular identification. Only six isolates (CM1, CM2, CM3, GM1, GM2, GM3) belong to Lactobacillus spp. with two sizes of amplicons which are 710 bp and 920 bp. All six isolates were considered to be bile tolerant with more than 50 % survival in 0. 3 % bile salt and their AST results displayed resistance towards sulfamethoxazole/trimethoprim, gentamicin and vancomycin. In conclusion, this research successfully identified six isolates of potentially probiotics Lactobacillus spp. from cow’s and goat’s milk. 2019-07 Thesis NonPeerReviewed text en https://ir.uitm.edu.my/id/eprint/50948/1/50948.pdf ID50948 Mohd Ibrahim, Noor 'Iffah Hanani (2019) The use of molecular tool in identifying potentially probiotic lactobacillus spp. isolated from cow's and goat's milk / Noor 'Iffah Hanani Mohd Ibrahim. Degree thesis, thesis, Universiti Teknologi MARA ( Kampus Puncak Alam ).
institution Universiti Teknologi Mara
building Tun Abdul Razak Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Teknologi Mara
content_source UiTM Institutional Repository
url_provider http://ir.uitm.edu.my/
language English
topic Immunology
Bacteria
Personal health and hygiene. Including clothing, bathing, exercise, travel, nutrition, sleep, sex hygiene
Cows
spellingShingle Immunology
Bacteria
Personal health and hygiene. Including clothing, bathing, exercise, travel, nutrition, sleep, sex hygiene
Cows
Mohd Ibrahim, Noor 'Iffah Hanani
The use of molecular tool in identifying potentially probiotic lactobacillus spp. isolated from cow's and goat's milk / Noor 'Iffah Hanani Mohd Ibrahim
description The interest in consuming products which contained probiotic especially Lactobacillus spp. are at increasing trend since they have been proved to offer numbers of health benefit on human. At present, Lactobacillus spp. are known to exist in many different sources but studies on those sources remain limited in Malaysia. Hence, investigation on two sources of potentially probiotic lactobacilli which are cow’s and goat’s milk obtained from local farms in Malaysia was conducted. This research focused on isolating and identifying potentially probiotic Lactobacillus spp. from cow’s and goat’s milk using both phenotypic and genotypic characterization. Initially, isolates from both samples were selected by phenotypic identification and further identified by molecular technique. DNA of isolates were extracted using NucleoSpin® Microbial DNA kit. Next, PCR amplification of 16S rRNA gene was performed using forward primer, 5' - GCT GGA TCA CCT CCT TTC - 3' and reverse primer, 5' - CCT TTC CCT CAC GGT ACT G - 3'. Amplicons were detected through 1.2 % agarose gel electrophoresis and visualized using ImageQuantᵀᴹ LAS 500. Bile salt tolerance was then performed to screen for probiotic property of six chosen isolates and their safety profiling was conducted through antibiotic susceptibility testing (AST). A total of 11 isolates revealed to be Gram-positive rod, negative for catalase and oxidase test were subjected to molecular identification. Only six isolates (CM1, CM2, CM3, GM1, GM2, GM3) belong to Lactobacillus spp. with two sizes of amplicons which are 710 bp and 920 bp. All six isolates were considered to be bile tolerant with more than 50 % survival in 0. 3 % bile salt and their AST results displayed resistance towards sulfamethoxazole/trimethoprim, gentamicin and vancomycin. In conclusion, this research successfully identified six isolates of potentially probiotics Lactobacillus spp. from cow’s and goat’s milk.
format Thesis
author Mohd Ibrahim, Noor 'Iffah Hanani
author_facet Mohd Ibrahim, Noor 'Iffah Hanani
author_sort Mohd Ibrahim, Noor 'Iffah Hanani
title The use of molecular tool in identifying potentially probiotic lactobacillus spp. isolated from cow's and goat's milk / Noor 'Iffah Hanani Mohd Ibrahim
title_short The use of molecular tool in identifying potentially probiotic lactobacillus spp. isolated from cow's and goat's milk / Noor 'Iffah Hanani Mohd Ibrahim
title_full The use of molecular tool in identifying potentially probiotic lactobacillus spp. isolated from cow's and goat's milk / Noor 'Iffah Hanani Mohd Ibrahim
title_fullStr The use of molecular tool in identifying potentially probiotic lactobacillus spp. isolated from cow's and goat's milk / Noor 'Iffah Hanani Mohd Ibrahim
title_full_unstemmed The use of molecular tool in identifying potentially probiotic lactobacillus spp. isolated from cow's and goat's milk / Noor 'Iffah Hanani Mohd Ibrahim
title_sort use of molecular tool in identifying potentially probiotic lactobacillus spp. isolated from cow's and goat's milk / noor 'iffah hanani mohd ibrahim
publishDate 2019
url https://ir.uitm.edu.my/id/eprint/50948/1/50948.pdf
https://ir.uitm.edu.my/id/eprint/50948/
_version_ 1712288431706472448
score 13.211869

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