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Phytochemical profiles and antimicrobial activity of Citrus hystrix DC. (Kaffir Lime) leaves extract against selected bacterial gastroenteritis / Nur Fatin Nadira Muda

In the recent decade, search of complementary and alternative plant derived medicine has gained gradually interest among researchers due to side effects of the chemically synthesized drugs and antibiotic resistant bacteria. This study was carried out to determine the phytochemical compounds and anti...

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Main Author: Muda, Nur Fatin Nadira
Format: Student Project
Language:English
Published: 2019
Subjects:
Botanical chemistry. Phytochemicals
Antibacterial agents
Fruit and fruit culture
Online Access:https://ir.uitm.edu.my/id/eprint/50575/1/50575.pdf
https://ir.uitm.edu.my/id/eprint/50575/
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spelling my.uitm.ir.505752021-09-20T08:32:49Z https://ir.uitm.edu.my/id/eprint/50575/ Phytochemical profiles and antimicrobial activity of Citrus hystrix DC. (Kaffir Lime) leaves extract against selected bacterial gastroenteritis / Nur Fatin Nadira Muda Muda, Nur Fatin Nadira Botanical chemistry. Phytochemicals Antibacterial agents Fruit and fruit culture In the recent decade, search of complementary and alternative plant derived medicine has gained gradually interest among researchers due to side effects of the chemically synthesized drugs and antibiotic resistant bacteria. This study was carried out to determine the phytochemical compounds and antibacterial activity of methanolic leaves extract of Citrus hystrix (C. hystrix) on selected gastrointestinal pathogens (Staphylococcus aureus, Bacillus cereus, Salmonella typhimurium and Shigella flexneri) using the agar well diffusion method. The extract exhibited broad spectrum antimicrobial activity against the tested bacteria ranging from 11 to 18 mm. Predominantly, the maximum inhibition zone was observed against B. cereus (17.33±0.58 mm) followed by S. aureus (15.67±0.58 mm). The extract showed moderate antibacterial activity against S. flexneri (11.67±0.58 mm) followed by S. typhimurium (11.33±0.58 mm). The strongest minimum inhibitory concentration (MIC) value was observed against B. cereus with concentration 31.25 mg/ml. The extract also showed good patterns of inhibition against S. aureus and S. typhimurium with concentration 125 mg/ml. MIC values were maximized against S. flexneri with concentration 250 mg/ml. The most effective bactericidal activity of the extract was observed against B. cereus with minimum bactericidal concentration (MBC) at 125 mg/ml followed by S. aureus and S. flexneri with MBC at 250 mg/ml. S. typhimurium was the least sensitive to the extract with the MBC value at 500 mg/ml. Phytochemical screenings revealed the presence of alkaloid, flavonoid, terpenoid and tannin compounds. The results of the present study suggest this extract exhibited the strongest antibacterial activity against B. cereus with maximum inhibition zone and least MIC and MBC values. 2019 Student Project NonPeerReviewed text en https://ir.uitm.edu.my/id/eprint/50575/1/50575.pdf ID50575 Muda, Nur Fatin Nadira (2019) Phytochemical profiles and antimicrobial activity of Citrus hystrix DC. (Kaffir Lime) leaves extract against selected bacterial gastroenteritis / Nur Fatin Nadira Muda. [Student Project] (Unpublished)
institution Universiti Teknologi Mara
building Tun Abdul Razak Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Teknologi Mara
content_source UiTM Institutional Repository
url_provider http://ir.uitm.edu.my/
language English
topic Botanical chemistry. Phytochemicals
Antibacterial agents
Fruit and fruit culture
spellingShingle Botanical chemistry. Phytochemicals
Antibacterial agents
Fruit and fruit culture
Muda, Nur Fatin Nadira
Phytochemical profiles and antimicrobial activity of Citrus hystrix DC. (Kaffir Lime) leaves extract against selected bacterial gastroenteritis / Nur Fatin Nadira Muda
description In the recent decade, search of complementary and alternative plant derived medicine has gained gradually interest among researchers due to side effects of the chemically synthesized drugs and antibiotic resistant bacteria. This study was carried out to determine the phytochemical compounds and antibacterial activity of methanolic leaves extract of Citrus hystrix (C. hystrix) on selected gastrointestinal pathogens (Staphylococcus aureus, Bacillus cereus, Salmonella typhimurium and Shigella flexneri) using the agar well diffusion method. The extract exhibited broad spectrum antimicrobial activity against the tested bacteria ranging from 11 to 18 mm. Predominantly, the maximum inhibition zone was observed against B. cereus (17.33±0.58 mm) followed by S. aureus (15.67±0.58 mm). The extract showed moderate antibacterial activity against S. flexneri (11.67±0.58 mm) followed by S. typhimurium (11.33±0.58 mm). The strongest minimum inhibitory concentration (MIC) value was observed against B. cereus with concentration 31.25 mg/ml. The extract also showed good patterns of inhibition against S. aureus and S. typhimurium with concentration 125 mg/ml. MIC values were maximized against S. flexneri with concentration 250 mg/ml. The most effective bactericidal activity of the extract was observed against B. cereus with minimum bactericidal concentration (MBC) at 125 mg/ml followed by S. aureus and S. flexneri with MBC at 250 mg/ml. S. typhimurium was the least sensitive to the extract with the MBC value at 500 mg/ml. Phytochemical screenings revealed the presence of alkaloid, flavonoid, terpenoid and tannin compounds. The results of the present study suggest this extract exhibited the strongest antibacterial activity against B. cereus with maximum inhibition zone and least MIC and MBC values.
format Student Project
author Muda, Nur Fatin Nadira
author_facet Muda, Nur Fatin Nadira
author_sort Muda, Nur Fatin Nadira
title Phytochemical profiles and antimicrobial activity of Citrus hystrix DC. (Kaffir Lime) leaves extract against selected bacterial gastroenteritis / Nur Fatin Nadira Muda
title_short Phytochemical profiles and antimicrobial activity of Citrus hystrix DC. (Kaffir Lime) leaves extract against selected bacterial gastroenteritis / Nur Fatin Nadira Muda
title_full Phytochemical profiles and antimicrobial activity of Citrus hystrix DC. (Kaffir Lime) leaves extract against selected bacterial gastroenteritis / Nur Fatin Nadira Muda
title_fullStr Phytochemical profiles and antimicrobial activity of Citrus hystrix DC. (Kaffir Lime) leaves extract against selected bacterial gastroenteritis / Nur Fatin Nadira Muda
title_full_unstemmed Phytochemical profiles and antimicrobial activity of Citrus hystrix DC. (Kaffir Lime) leaves extract against selected bacterial gastroenteritis / Nur Fatin Nadira Muda
title_sort phytochemical profiles and antimicrobial activity of citrus hystrix dc. (kaffir lime) leaves extract against selected bacterial gastroenteritis / nur fatin nadira muda
publishDate 2019
url https://ir.uitm.edu.my/id/eprint/50575/1/50575.pdf
https://ir.uitm.edu.my/id/eprint/50575/
_version_ 1712288397427474432
score 13.211869

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