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Characterization of airborne fungi isolated from Faculty of Dentistry in UiTM Sungai Buloh / Nurina Hannani Hishammuddin

Indoor airborne fungi has been recognized as contributor to irritative disorders, primarily non-infective diseases such as allergy and asthma. Spores and hyphal fragments of fungi contained in bioaerosols can be breathed in easily and evoke bronchial irritation and allergy and in immunocompromised i...

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Main Author: Hishammuddin, Nurina Hannani
Format: Student Project
Language:English
Published: 2019
Subjects:
Cryptogams
Organic substances
Transmission of disease
Online Access:https://ir.uitm.edu.my/id/eprint/50574/1/50574.pdf
https://ir.uitm.edu.my/id/eprint/50574/
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spelling my.uitm.ir.505742021-09-17T06:49:50Z https://ir.uitm.edu.my/id/eprint/50574/ Characterization of airborne fungi isolated from Faculty of Dentistry in UiTM Sungai Buloh / Nurina Hannani Hishammuddin Hishammuddin, Nurina Hannani Cryptogams Organic substances Transmission of disease Indoor airborne fungi has been recognized as contributor to irritative disorders, primarily non-infective diseases such as allergy and asthma. Spores and hyphal fragments of fungi contained in bioaerosols can be breathed in easily and evoke bronchial irritation and allergy and in immunocompromised individuals, fungi can cause opportunistic infections. Therefore, the present study was undertaken to characterize the airborne fungi that have been isolated from the environments of Faculty of Dentistry in UiTM Sungai Buloh campus by their macromorphology, micromorphology and also molecular characteristics. Fungi that were previously kept in 20% glycerol stock were cultured back on Sabouraud dextrose agar (SDA) and were observed for their macromorphological and micromorphological characteristics. DNA was then extracted and the internal transcribed spacer regions (ITS1-5.8S-ITS2) was amplified using forward primer ITS1, 5’-TCC GTA GGT GAA CCT GCG G-3’ and reverse primer ITS4, 5’-TCC TCC GCT TAT TGA TAT GC-3’. The amplified products were detected on 1% agarose gel, viewed using a gel documentation system, ImageQuant LAS500, sent for sequencing and results were analyzed using the National Center for Biotechnology Information (NCBI) database. The macromorphology of the fungal isolates were successfully observed based on colour, texture and form while the micromorphology were looked for morphological structures such as phialides and metulae. The nucleotide sequence results received were run through Basic Local Alignment Search Tool (BLAST) and one of the airborne fungi that were isolated was detected as Penicillium citrinum with 99% sequence similarity. In conclusion, a combination of macromorphological, micromorphological and molecular characteristics is useful for characterization of airborne fung 2019 Student Project NonPeerReviewed text en https://ir.uitm.edu.my/id/eprint/50574/1/50574.pdf ID50574 Hishammuddin, Nurina Hannani (2019) Characterization of airborne fungi isolated from Faculty of Dentistry in UiTM Sungai Buloh / Nurina Hannani Hishammuddin. [Student Project] (Unpublished)
institution Universiti Teknologi Mara
building Tun Abdul Razak Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Teknologi Mara
content_source UiTM Institutional Repository
url_provider http://ir.uitm.edu.my/
language English
topic Cryptogams
Organic substances
Transmission of disease
spellingShingle Cryptogams
Organic substances
Transmission of disease
Hishammuddin, Nurina Hannani
Characterization of airborne fungi isolated from Faculty of Dentistry in UiTM Sungai Buloh / Nurina Hannani Hishammuddin
description Indoor airborne fungi has been recognized as contributor to irritative disorders, primarily non-infective diseases such as allergy and asthma. Spores and hyphal fragments of fungi contained in bioaerosols can be breathed in easily and evoke bronchial irritation and allergy and in immunocompromised individuals, fungi can cause opportunistic infections. Therefore, the present study was undertaken to characterize the airborne fungi that have been isolated from the environments of Faculty of Dentistry in UiTM Sungai Buloh campus by their macromorphology, micromorphology and also molecular characteristics. Fungi that were previously kept in 20% glycerol stock were cultured back on Sabouraud dextrose agar (SDA) and were observed for their macromorphological and micromorphological characteristics. DNA was then extracted and the internal transcribed spacer regions (ITS1-5.8S-ITS2) was amplified using forward primer ITS1, 5’-TCC GTA GGT GAA CCT GCG G-3’ and reverse primer ITS4, 5’-TCC TCC GCT TAT TGA TAT GC-3’. The amplified products were detected on 1% agarose gel, viewed using a gel documentation system, ImageQuant LAS500, sent for sequencing and results were analyzed using the National Center for Biotechnology Information (NCBI) database. The macromorphology of the fungal isolates were successfully observed based on colour, texture and form while the micromorphology were looked for morphological structures such as phialides and metulae. The nucleotide sequence results received were run through Basic Local Alignment Search Tool (BLAST) and one of the airborne fungi that were isolated was detected as Penicillium citrinum with 99% sequence similarity. In conclusion, a combination of macromorphological, micromorphological and molecular characteristics is useful for characterization of airborne fung
format Student Project
author Hishammuddin, Nurina Hannani
author_facet Hishammuddin, Nurina Hannani
author_sort Hishammuddin, Nurina Hannani
title Characterization of airborne fungi isolated from Faculty of Dentistry in UiTM Sungai Buloh / Nurina Hannani Hishammuddin
title_short Characterization of airborne fungi isolated from Faculty of Dentistry in UiTM Sungai Buloh / Nurina Hannani Hishammuddin
title_full Characterization of airborne fungi isolated from Faculty of Dentistry in UiTM Sungai Buloh / Nurina Hannani Hishammuddin
title_fullStr Characterization of airborne fungi isolated from Faculty of Dentistry in UiTM Sungai Buloh / Nurina Hannani Hishammuddin
title_full_unstemmed Characterization of airborne fungi isolated from Faculty of Dentistry in UiTM Sungai Buloh / Nurina Hannani Hishammuddin
title_sort characterization of airborne fungi isolated from faculty of dentistry in uitm sungai buloh / nurina hannani hishammuddin
publishDate 2019
url https://ir.uitm.edu.my/id/eprint/50574/1/50574.pdf
https://ir.uitm.edu.my/id/eprint/50574/
_version_ 1712288397280673792
score 13.211869

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