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Isolation of endophytic actinomycetes from psidium guajava and ziziphus mauritiana / Nurul Najwa Saleh

Endophytic microorganisms are usually bacteria, fungi or actinomycetes that inhabit the internal part of plants without causing apparent symptoms of a disease to the plants. Actinomycetes have been used for drug discovery because it produces more than 10, 000 bioactive compounds. Endophytic actinomy...

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Main Author: Saleh, Nurul Najwa
Format: Student Project
Language:English
Published: 2019
Subjects:
Cryptogams
Botanical chemistry. Phytochemicals
Fruit and fruit culture
Online Access:https://ir.uitm.edu.my/id/eprint/50572/1/50572.pdf
https://ir.uitm.edu.my/id/eprint/50572/
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spelling my.uitm.ir.505722021-09-21T02:08:15Z https://ir.uitm.edu.my/id/eprint/50572/ Isolation of endophytic actinomycetes from psidium guajava and ziziphus mauritiana / Nurul Najwa Saleh Saleh, Nurul Najwa Cryptogams Botanical chemistry. Phytochemicals Fruit and fruit culture Endophytic microorganisms are usually bacteria, fungi or actinomycetes that inhabit the internal part of plants without causing apparent symptoms of a disease to the plants. Actinomycetes have been used for drug discovery because it produces more than 10, 000 bioactive compounds. Endophytic actinomycetes possess metabolites with a broadspectrum activity where it can be used to treat multidrug-resistant pathogens, such as Methicillin resistant Staphylococcus aureus (MRSA) whereby ethnomedicinal plants is an essential resource of isolating these endophytic actinomycetes. Therefore, the present study was designed to correlate the presence of endophytic actinomycetes in ethnomedicinal plants by isolating them from Psidium guajava and Ziziphus mauritiana. The efficacy of two surface sterilization methods and two different incubation temperatures for isolation of endophytic actinomycetes were assessed by using low based media. Two procedures were used for surface sterilization, Procedure I using 70% ethanol and Procedure II using 99% ethanol and the isolation plate was then incubated at 30°C and 37°C for one month. The method using 99% ethanol (Procedure II) in surface sterilization procedure was observed more effective in eliminating the epiphytes and incubation at 37°C was found to be practical for the isolation of endophytic actinomycetes. Based on morphological characteristics, all seven isolates were identified with Streptomyces spp and were isolated from roots of plants. From the seven isolated, 71.4% (n=5) were isolated from Z. mauritiana and 28.6% (n=2) from P. guajava. In conclusion, the isolation and optimization to enhance the growth of endophytes through surface sterilization and incubation temperature was described and it shows that the endophytic actinomycetes can potentially be harvested from ethnomedicinal plants. 2019 Student Project NonPeerReviewed text en https://ir.uitm.edu.my/id/eprint/50572/1/50572.pdf ID50572 Saleh, Nurul Najwa (2019) Isolation of endophytic actinomycetes from psidium guajava and ziziphus mauritiana / Nurul Najwa Saleh. [Student Project] (Unpublished)
institution Universiti Teknologi Mara
building Tun Abdul Razak Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Teknologi Mara
content_source UiTM Institutional Repository
url_provider http://ir.uitm.edu.my/
language English
topic Cryptogams
Botanical chemistry. Phytochemicals
Fruit and fruit culture
spellingShingle Cryptogams
Botanical chemistry. Phytochemicals
Fruit and fruit culture
Saleh, Nurul Najwa
Isolation of endophytic actinomycetes from psidium guajava and ziziphus mauritiana / Nurul Najwa Saleh
description Endophytic microorganisms are usually bacteria, fungi or actinomycetes that inhabit the internal part of plants without causing apparent symptoms of a disease to the plants. Actinomycetes have been used for drug discovery because it produces more than 10, 000 bioactive compounds. Endophytic actinomycetes possess metabolites with a broadspectrum activity where it can be used to treat multidrug-resistant pathogens, such as Methicillin resistant Staphylococcus aureus (MRSA) whereby ethnomedicinal plants is an essential resource of isolating these endophytic actinomycetes. Therefore, the present study was designed to correlate the presence of endophytic actinomycetes in ethnomedicinal plants by isolating them from Psidium guajava and Ziziphus mauritiana. The efficacy of two surface sterilization methods and two different incubation temperatures for isolation of endophytic actinomycetes were assessed by using low based media. Two procedures were used for surface sterilization, Procedure I using 70% ethanol and Procedure II using 99% ethanol and the isolation plate was then incubated at 30°C and 37°C for one month. The method using 99% ethanol (Procedure II) in surface sterilization procedure was observed more effective in eliminating the epiphytes and incubation at 37°C was found to be practical for the isolation of endophytic actinomycetes. Based on morphological characteristics, all seven isolates were identified with Streptomyces spp and were isolated from roots of plants. From the seven isolated, 71.4% (n=5) were isolated from Z. mauritiana and 28.6% (n=2) from P. guajava. In conclusion, the isolation and optimization to enhance the growth of endophytes through surface sterilization and incubation temperature was described and it shows that the endophytic actinomycetes can potentially be harvested from ethnomedicinal plants.
format Student Project
author Saleh, Nurul Najwa
author_facet Saleh, Nurul Najwa
author_sort Saleh, Nurul Najwa
title Isolation of endophytic actinomycetes from psidium guajava and ziziphus mauritiana / Nurul Najwa Saleh
title_short Isolation of endophytic actinomycetes from psidium guajava and ziziphus mauritiana / Nurul Najwa Saleh
title_full Isolation of endophytic actinomycetes from psidium guajava and ziziphus mauritiana / Nurul Najwa Saleh
title_fullStr Isolation of endophytic actinomycetes from psidium guajava and ziziphus mauritiana / Nurul Najwa Saleh
title_full_unstemmed Isolation of endophytic actinomycetes from psidium guajava and ziziphus mauritiana / Nurul Najwa Saleh
title_sort isolation of endophytic actinomycetes from psidium guajava and ziziphus mauritiana / nurul najwa saleh
publishDate 2019
url https://ir.uitm.edu.my/id/eprint/50572/1/50572.pdf
https://ir.uitm.edu.my/id/eprint/50572/
_version_ 1712288396996509696
score 13.211869

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