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Detection of intercellular adhesion genes: the virulence genes in biofilm formation of staphylococcus epidermidis / Fazlin Haron

Staphylococcus epidermidis (S. epidermidis) is a natural constituent of the human skin and mucosal surfaces. Nowadays, S. epidermidis are recognized as opportunistic human pathogens and are widespread in the environment. Generally, the success of S. epidermidis as a pathogen has to be attributed to...

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Main Author: Haron, Fazlin
Format: Thesis
Language:English
Published: 2017
Subjects:
Atomic physics. Constitution and properties of matter
Microbial ecology
Miscellaneous organic chemical industries
Online Access:https://ir.uitm.edu.my/id/eprint/50138/1/50138.pdf
https://ir.uitm.edu.my/id/eprint/50138/
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spelling my.uitm.ir.501382021-09-06T09:29:21Z https://ir.uitm.edu.my/id/eprint/50138/ Detection of intercellular adhesion genes: the virulence genes in biofilm formation of staphylococcus epidermidis / Fazlin Haron Haron, Fazlin Atomic physics. Constitution and properties of matter Microbial ecology Miscellaneous organic chemical industries Staphylococcus epidermidis (S. epidermidis) is a natural constituent of the human skin and mucosal surfaces. Nowadays, S. epidermidis are recognized as opportunistic human pathogens and are widespread in the environment. Generally, the success of S. epidermidis as a pathogen has to be attributed to its ability to adhere to surfaces and to remain there under the cover of a protection extracellular material in relative silence. The intercellular adhesion (ica) operon genes are the virulence genes of S. epidermidis that consist of icaA, icaB, icaC and icaD respectively. This study only focusing on two ica genes (icaA, icaD). Biofilm formation of S. epidermidis is mediated by the expression of the ica genes operon. Thus, this research aim to detect the intercellular adhesion (ica) genes in biofilm formation of S. epidermidis. In this study, twenty-one samples of S. epidermidis were sub-cultured from previous study samples. Several identification test were performed to identify and confirm S. epidermidis spp. from the samples. Detection of targeting icaA and icaD genes were performed by using real-time polymerase chain reaction (qPCR). Based on the result obtained, from twenty-one samples tested only five (23.8%) expressed the icaA genes. The remaining 16 (76.2 %) samples showed no genes were observed. The result for icaD genes was non-specific from twenty-one samples that performed qPCR. Among ica genes, icaA and icaD have been reported to play a significant role in biofilm formation in S. epidermidis. It is significant to note that both genes were demonstrated in biofilm producing strains of S. epidermidis although there were no icaD genes observed in this study. Further research is needed to discover more specific virulence properties of ica operon genes and gain awareness about biofilm formation that occurred not only on medical devices but also in human body. 2017 Thesis NonPeerReviewed text en https://ir.uitm.edu.my/id/eprint/50138/1/50138.pdf ID50138 Haron, Fazlin (2017) Detection of intercellular adhesion genes: the virulence genes in biofilm formation of staphylococcus epidermidis / Fazlin Haron. Degree thesis, thesis, Universiti Teknologi MARA (Kampus Puncak Alam).
institution Universiti Teknologi Mara
building Tun Abdul Razak Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Teknologi Mara
content_source UiTM Institutional Repository
url_provider http://ir.uitm.edu.my/
language English
topic Atomic physics. Constitution and properties of matter
Microbial ecology
Miscellaneous organic chemical industries
spellingShingle Atomic physics. Constitution and properties of matter
Microbial ecology
Miscellaneous organic chemical industries
Haron, Fazlin
Detection of intercellular adhesion genes: the virulence genes in biofilm formation of staphylococcus epidermidis / Fazlin Haron
description Staphylococcus epidermidis (S. epidermidis) is a natural constituent of the human skin and mucosal surfaces. Nowadays, S. epidermidis are recognized as opportunistic human pathogens and are widespread in the environment. Generally, the success of S. epidermidis as a pathogen has to be attributed to its ability to adhere to surfaces and to remain there under the cover of a protection extracellular material in relative silence. The intercellular adhesion (ica) operon genes are the virulence genes of S. epidermidis that consist of icaA, icaB, icaC and icaD respectively. This study only focusing on two ica genes (icaA, icaD). Biofilm formation of S. epidermidis is mediated by the expression of the ica genes operon. Thus, this research aim to detect the intercellular adhesion (ica) genes in biofilm formation of S. epidermidis. In this study, twenty-one samples of S. epidermidis were sub-cultured from previous study samples. Several identification test were performed to identify and confirm S. epidermidis spp. from the samples. Detection of targeting icaA and icaD genes were performed by using real-time polymerase chain reaction (qPCR). Based on the result obtained, from twenty-one samples tested only five (23.8%) expressed the icaA genes. The remaining 16 (76.2 %) samples showed no genes were observed. The result for icaD genes was non-specific from twenty-one samples that performed qPCR. Among ica genes, icaA and icaD have been reported to play a significant role in biofilm formation in S. epidermidis. It is significant to note that both genes were demonstrated in biofilm producing strains of S. epidermidis although there were no icaD genes observed in this study. Further research is needed to discover more specific virulence properties of ica operon genes and gain awareness about biofilm formation that occurred not only on medical devices but also in human body.
format Thesis
author Haron, Fazlin
author_facet Haron, Fazlin
author_sort Haron, Fazlin
title Detection of intercellular adhesion genes: the virulence genes in biofilm formation of staphylococcus epidermidis / Fazlin Haron
title_short Detection of intercellular adhesion genes: the virulence genes in biofilm formation of staphylococcus epidermidis / Fazlin Haron
title_full Detection of intercellular adhesion genes: the virulence genes in biofilm formation of staphylococcus epidermidis / Fazlin Haron
title_fullStr Detection of intercellular adhesion genes: the virulence genes in biofilm formation of staphylococcus epidermidis / Fazlin Haron
title_full_unstemmed Detection of intercellular adhesion genes: the virulence genes in biofilm formation of staphylococcus epidermidis / Fazlin Haron
title_sort detection of intercellular adhesion genes: the virulence genes in biofilm formation of staphylococcus epidermidis / fazlin haron
publishDate 2017
url https://ir.uitm.edu.my/id/eprint/50138/1/50138.pdf
https://ir.uitm.edu.my/id/eprint/50138/
_version_ 1710678958329036800
score 13.211869

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