Qualitative phytochemical screening and antibacterial effect of averrhoa bilimbi fruits extracts against foodborne pathogens / Faizah Saniah Ibrahim

Foodborne diseases have cause high mortality and morbidity worldwide. However, the use and misuse of synthetics antibiotics in treating the diseases not only cause the adverse effects to the patient but can also cause the emergence of enteropathogens. Thus, this study was conducted to determine the...

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Main Author: Ibrahim, Faizah Saniah
Format: Thesis
Language:English
Published: 2017
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Online Access:https://ir.uitm.edu.my/id/eprint/50134/1/50134.pdf
https://ir.uitm.edu.my/id/eprint/50134/
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spelling my.uitm.ir.501342021-09-06T08:41:37Z https://ir.uitm.edu.my/id/eprint/50134/ Qualitative phytochemical screening and antibacterial effect of averrhoa bilimbi fruits extracts against foodborne pathogens / Faizah Saniah Ibrahim Ibrahim, Faizah Saniah Botanical chemistry. Phytochemicals Foodborne diseases Antibacterial agents Foodborne diseases have cause high mortality and morbidity worldwide. However, the use and misuse of synthetics antibiotics in treating the diseases not only cause the adverse effects to the patient but can also cause the emergence of enteropathogens. Thus, this study was conducted to determine the potential of Averrhoa bilimbi fruits extracts as natural antibacterial agents against foodborne pathogens as a substitute for the synthetic drugs. Antimicrobial Susceptibility Testing (AST) by disc diffusion method and Minimum Inhibitory Concentration (MIC) by microdilution broth method were performed to evaluate the antibacterial activity of the extracts against 2 types of Gram-positive and Gram-negative bacteria. They were Staphylococcus aureus, Bacillus cereus, Escherichia coli and Salmonella typhimurium. The phytochemical compounds of the extracts were interpreted by observing the color changes produced when the extracts were mixed with chemical reagents. Preliminary screening for ethyl acetate and aqueous extracts showed inhibition activity against all organisms with the greatest effect on Bacillus cereus (16 and 11.67±1.15 mm, respectively). Staphylococcus aureus and Bacillus cereus required the lowest concentration of ethyl acetate extracts to inhibit their growth with MIC value of 7.81 mg/ml. Lowest MIC value, 31.25 mg/ml of aqueous extract was observed to inhibit Escherichia coli. Moreover, alkaloids, flavonoids, phenols, terpenoids, tannins and reducing sugars that responsible for antibacterial activity were found in Averrhoa bilimbi fruits extracts. Thus, Averrhoa bilimbi fruits extract are potential to be used as a natural antimicrobial agent to treat the foodborne diseases. However, the in vivo study must be conducted to assess the cytotoxic and biological effects towards human prior to usage. 2017 Thesis NonPeerReviewed text en https://ir.uitm.edu.my/id/eprint/50134/1/50134.pdf ID50134 Ibrahim, Faizah Saniah (2017) Qualitative phytochemical screening and antibacterial effect of averrhoa bilimbi fruits extracts against foodborne pathogens / Faizah Saniah Ibrahim. Degree thesis, thesis, Universiti Teknologi MARA (Kampus Puncak Alam).
institution Universiti Teknologi Mara
building Tun Abdul Razak Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Teknologi Mara
content_source UiTM Institutional Repository
url_provider http://ir.uitm.edu.my/
language English
topic Botanical chemistry. Phytochemicals
Foodborne diseases
Antibacterial agents
spellingShingle Botanical chemistry. Phytochemicals
Foodborne diseases
Antibacterial agents
Ibrahim, Faizah Saniah
Qualitative phytochemical screening and antibacterial effect of averrhoa bilimbi fruits extracts against foodborne pathogens / Faizah Saniah Ibrahim
description Foodborne diseases have cause high mortality and morbidity worldwide. However, the use and misuse of synthetics antibiotics in treating the diseases not only cause the adverse effects to the patient but can also cause the emergence of enteropathogens. Thus, this study was conducted to determine the potential of Averrhoa bilimbi fruits extracts as natural antibacterial agents against foodborne pathogens as a substitute for the synthetic drugs. Antimicrobial Susceptibility Testing (AST) by disc diffusion method and Minimum Inhibitory Concentration (MIC) by microdilution broth method were performed to evaluate the antibacterial activity of the extracts against 2 types of Gram-positive and Gram-negative bacteria. They were Staphylococcus aureus, Bacillus cereus, Escherichia coli and Salmonella typhimurium. The phytochemical compounds of the extracts were interpreted by observing the color changes produced when the extracts were mixed with chemical reagents. Preliminary screening for ethyl acetate and aqueous extracts showed inhibition activity against all organisms with the greatest effect on Bacillus cereus (16 and 11.67±1.15 mm, respectively). Staphylococcus aureus and Bacillus cereus required the lowest concentration of ethyl acetate extracts to inhibit their growth with MIC value of 7.81 mg/ml. Lowest MIC value, 31.25 mg/ml of aqueous extract was observed to inhibit Escherichia coli. Moreover, alkaloids, flavonoids, phenols, terpenoids, tannins and reducing sugars that responsible for antibacterial activity were found in Averrhoa bilimbi fruits extracts. Thus, Averrhoa bilimbi fruits extract are potential to be used as a natural antimicrobial agent to treat the foodborne diseases. However, the in vivo study must be conducted to assess the cytotoxic and biological effects towards human prior to usage.
format Thesis
author Ibrahim, Faizah Saniah
author_facet Ibrahim, Faizah Saniah
author_sort Ibrahim, Faizah Saniah
title Qualitative phytochemical screening and antibacterial effect of averrhoa bilimbi fruits extracts against foodborne pathogens / Faizah Saniah Ibrahim
title_short Qualitative phytochemical screening and antibacterial effect of averrhoa bilimbi fruits extracts against foodborne pathogens / Faizah Saniah Ibrahim
title_full Qualitative phytochemical screening and antibacterial effect of averrhoa bilimbi fruits extracts against foodborne pathogens / Faizah Saniah Ibrahim
title_fullStr Qualitative phytochemical screening and antibacterial effect of averrhoa bilimbi fruits extracts against foodborne pathogens / Faizah Saniah Ibrahim
title_full_unstemmed Qualitative phytochemical screening and antibacterial effect of averrhoa bilimbi fruits extracts against foodborne pathogens / Faizah Saniah Ibrahim
title_sort qualitative phytochemical screening and antibacterial effect of averrhoa bilimbi fruits extracts against foodborne pathogens / faizah saniah ibrahim
publishDate 2017
url https://ir.uitm.edu.my/id/eprint/50134/1/50134.pdf
https://ir.uitm.edu.my/id/eprint/50134/
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score 13.211869