Staff View: Phytochemicals from the stem bark of Calophyllum ferrugineum Ridl. and Calophyllum andersonii P. F. Stevens and their bioassays / Izzah Afifah Noh

Phytochemicals from the stem bark of Calophyllum ferrugineum Ridl. and Calophyllum andersonii P. F. Stevens and their bioassays / Izzah Afifah Noh

Plant from the genus Calophyllum were known for its medicinal properties and used to treat swollen gums, arthritis, diarrhoea, chronic abscess, skin infections and lesion. The species, Calophyllum ferrugineum and Calophyllum andersonii from Sarawak has least studied about their phytochemical constit...

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Main Author:	Noh, Izzah Afifah
Format:	Thesis
Language:	English
Published:	2021
Subjects:	Growth. Development. Including pattern formation Botanical chemistry. Phytochemicals
Online Access:	https://ir.uitm.edu.my/id/eprint/46554/1/46554.pdf https://ir.uitm.edu.my/id/eprint/46554/
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spelling	my.uitm.ir.465542022-03-24T04:10:31Z https://ir.uitm.edu.my/id/eprint/46554/ Phytochemicals from the stem bark of Calophyllum ferrugineum Ridl. and Calophyllum andersonii P. F. Stevens and their bioassays / Izzah Afifah Noh Noh, Izzah Afifah Growth. Development. Including pattern formation Botanical chemistry. Phytochemicals Plant from the genus Calophyllum were known for its medicinal properties and used to treat swollen gums, arthritis, diarrhoea, chronic abscess, skin infections and lesion. The species, Calophyllum ferrugineum and Calophyllum andersonii from Sarawak has least studied about their phytochemical constituents and biological activities. The aims of this study are to isolate and identify the phytochemical constituents from both species and to determine the antibacterial and antioxidant activity. The plant stem barks were collected from Semenggoh Nature Reserve in Sarawak and underwent maceration process to extract the samples. The extracts were subjected to chromatographic methods for the isolation and purification processes. Structural elucidation was achieved by using IR, MS, 1D and 2D NMR. The further isolation process from both species by using column and centrifugal chromatography has afforded different classes of compounds such as triterpene, xanthone, coumarin and fatty acid. Calophyllum ferrugineum afforded four compounds from n-hexane, chloroform and methanol extracts namely friedelin (5), lupeol (100), 1-hydroxy-7- methoxyxanthone (77), isocalanone (11). Meanwhile, Calophyllum andersonii afforded seven compounds namely linoleic acid (91), oleic acid (90), thwaitesixanthone (84), euxanthone (64), pyranojacareubin (54) and mammea A/BB cyclo F (101) from n-hexane, chloroform and methanol extracts. There were 10 compounds in total from both species. Friedelin (5) was recorded presence in both species. Isocalanone and methanol extract of C. ferrugineum showed appreciable antioxidant activity with the IC50 values of 27 ± 5.23, 38 ± 7.04 and 34 ± 3.70 μg/mL compared to the positive control; quercetin (15.5 ± 1.38 μg/mL) and ascorbic acid (14.2 ± 1.21 μg/mL), respectively. The chloroform and methanol extracts from both species showed significant inhibitions against S. aureus, B. subtilis, P. aeruginosa and E. coli with MIC and MBC values ranging from 225 and 112.5 μg/mL compared to the positive control. The results obtained from this study highlighted the potential of both species as antibacterial and antioxidant agent. 2021 Thesis NonPeerReviewed text en https://ir.uitm.edu.my/id/eprint/46554/1/46554.pdf (2021) Phytochemicals from the stem bark of Calophyllum ferrugineum Ridl. and Calophyllum andersonii P. F. Stevens and their bioassays / Izzah Afifah Noh. Masters thesis, thesis, Universiti Teknologi MARA.
institution	Universiti Teknologi Mara
building	Tun Abdul Razak Library
collection	Institutional Repository
continent	Asia
country	Malaysia
content_provider	Universiti Teknologi Mara
content_source	UiTM Institutional Repository
url_provider	http://ir.uitm.edu.my/
language	English
topic	Growth. Development. Including pattern formation Botanical chemistry. Phytochemicals
spellingShingle	Growth. Development. Including pattern formation Botanical chemistry. Phytochemicals Noh, Izzah Afifah Phytochemicals from the stem bark of Calophyllum ferrugineum Ridl. and Calophyllum andersonii P. F. Stevens and their bioassays / Izzah Afifah Noh
description	Plant from the genus Calophyllum were known for its medicinal properties and used to treat swollen gums, arthritis, diarrhoea, chronic abscess, skin infections and lesion. The species, Calophyllum ferrugineum and Calophyllum andersonii from Sarawak has least studied about their phytochemical constituents and biological activities. The aims of this study are to isolate and identify the phytochemical constituents from both species and to determine the antibacterial and antioxidant activity. The plant stem barks were collected from Semenggoh Nature Reserve in Sarawak and underwent maceration process to extract the samples. The extracts were subjected to chromatographic methods for the isolation and purification processes. Structural elucidation was achieved by using IR, MS, 1D and 2D NMR. The further isolation process from both species by using column and centrifugal chromatography has afforded different classes of compounds such as triterpene, xanthone, coumarin and fatty acid. Calophyllum ferrugineum afforded four compounds from n-hexane, chloroform and methanol extracts namely friedelin (5), lupeol (100), 1-hydroxy-7- methoxyxanthone (77), isocalanone (11). Meanwhile, Calophyllum andersonii afforded seven compounds namely linoleic acid (91), oleic acid (90), thwaitesixanthone (84), euxanthone (64), pyranojacareubin (54) and mammea A/BB cyclo F (101) from n-hexane, chloroform and methanol extracts. There were 10 compounds in total from both species. Friedelin (5) was recorded presence in both species. Isocalanone and methanol extract of C. ferrugineum showed appreciable antioxidant activity with the IC50 values of 27 ± 5.23, 38 ± 7.04 and 34 ± 3.70 μg/mL compared to the positive control; quercetin (15.5 ± 1.38 μg/mL) and ascorbic acid (14.2 ± 1.21 μg/mL), respectively. The chloroform and methanol extracts from both species showed significant inhibitions against S. aureus, B. subtilis, P. aeruginosa and E. coli with MIC and MBC values ranging from 225 and 112.5 μg/mL compared to the positive control. The results obtained from this study highlighted the potential of both species as antibacterial and antioxidant agent.
format	Thesis
author	Noh, Izzah Afifah
author_facet	Noh, Izzah Afifah
author_sort	Noh, Izzah Afifah
title	Phytochemicals from the stem bark of Calophyllum ferrugineum Ridl. and Calophyllum andersonii P. F. Stevens and their bioassays / Izzah Afifah Noh
title_short	Phytochemicals from the stem bark of Calophyllum ferrugineum Ridl. and Calophyllum andersonii P. F. Stevens and their bioassays / Izzah Afifah Noh
title_full	Phytochemicals from the stem bark of Calophyllum ferrugineum Ridl. and Calophyllum andersonii P. F. Stevens and their bioassays / Izzah Afifah Noh
title_fullStr	Phytochemicals from the stem bark of Calophyllum ferrugineum Ridl. and Calophyllum andersonii P. F. Stevens and their bioassays / Izzah Afifah Noh
title_full_unstemmed	Phytochemicals from the stem bark of Calophyllum ferrugineum Ridl. and Calophyllum andersonii P. F. Stevens and their bioassays / Izzah Afifah Noh
title_sort	phytochemicals from the stem bark of calophyllum ferrugineum ridl. and calophyllum andersonii p. f. stevens and their bioassays / izzah afifah noh
publishDate	2021
url	https://ir.uitm.edu.my/id/eprint/46554/1/46554.pdf https://ir.uitm.edu.my/id/eprint/46554/
_version_	1729707469411713024
score	13.211869

Phytochemicals from the stem bark of Calophyllum ferrugineum Ridl. and Calophyllum andersonii P. F. Stevens and their bioassays / Izzah Afifah Noh

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