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Screening of magnetotactic bacteria from lakes in Universiti Sains Malaysia, Penang / Nurulatika Minhad

Magnetotactic bacteria (MTB) is a Gram-negative prokaryote which depends on the magnetic influence called magnetotaxis imposed upon them. MTB produces intracellular encased magnetic grains, magnetosomes. The bacteria are hard to isolate in axenic culture due to its fastidious lifestyle. The objectiv...

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Main Author: Minhad, Nurulatika
Format: Article
Language:English
Published: Universiti Teknologi MARA Cawangan Pahang 2020
Subjects:
Cultures and culture media. Culture technique.
Bacteria
Online Access:http://ir.uitm.edu.my/id/eprint/45934/1/45934.pdf
http://ir.uitm.edu.my/id/eprint/45934/
http://www.gadingst.learningdistance.org/index.php/gadingst/article/view/96
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spelling my.uitm.ir.459342021-06-18T01:36:36Z http://ir.uitm.edu.my/id/eprint/45934/ Screening of magnetotactic bacteria from lakes in Universiti Sains Malaysia, Penang / Nurulatika Minhad Minhad, Nurulatika Cultures and culture media. Culture technique. Bacteria Magnetotactic bacteria (MTB) is a Gram-negative prokaryote which depends on the magnetic influence called magnetotaxis imposed upon them. MTB produces intracellular encased magnetic grains, magnetosomes. The bacteria are hard to isolate in axenic culture due to its fastidious lifestyle. The objective of the study is to screen water samples from two lakes in Universiti Sains Malaysia (USM), Penang for magnetotactic bacteria. Approximately 500 microliter (ml) of samples were collected in triplicates and incubated in the dark for 2 days. For isolation of MTB, a modified method of racetrack purification, hanging drop and centrifugation technique of MTB were used. The samples were enriched in a modified medium and incubated in a shaker incubator for one week. Pour plate and streak plate methods were done. For characterization, microscopic analysis using optical and scanning electron microscope (SEM) was carried out to observe swimming behaviour of MTB and its magnetosomes if any. The screening of MTB showed that all the samples were Gram-negative but there was no movement of bacteria towards magnetic force and no magnetosomes found. Therefore, it can be assumed that water samples from the two lakes in USM do not have magnetotactic bacteria due to its fastidious growth, hard to isolate in axenic culture and exists in a place with abundant of soluble ferrous form. Universiti Teknologi MARA Cawangan Pahang 2020-09 Article PeerReviewed text en http://ir.uitm.edu.my/id/eprint/45934/1/45934.pdf ID45934 Minhad, Nurulatika (2020) Screening of magnetotactic bacteria from lakes in Universiti Sains Malaysia, Penang / Nurulatika Minhad. Gading Journal of Science and Technology, 3 (2). pp. 45-52. ISSN (e-ISSN) : 2637 - 0018 http://www.gadingst.learningdistance.org/index.php/gadingst/article/view/96
institution Universiti Teknologi Mara
building Tun Abdul Razak Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Teknologi Mara
content_source UiTM Institutional Repository
url_provider http://ir.uitm.edu.my/
language English
topic Cultures and culture media. Culture technique.
Bacteria
spellingShingle Cultures and culture media. Culture technique.
Bacteria
Minhad, Nurulatika
Screening of magnetotactic bacteria from lakes in Universiti Sains Malaysia, Penang / Nurulatika Minhad
description Magnetotactic bacteria (MTB) is a Gram-negative prokaryote which depends on the magnetic influence called magnetotaxis imposed upon them. MTB produces intracellular encased magnetic grains, magnetosomes. The bacteria are hard to isolate in axenic culture due to its fastidious lifestyle. The objective of the study is to screen water samples from two lakes in Universiti Sains Malaysia (USM), Penang for magnetotactic bacteria. Approximately 500 microliter (ml) of samples were collected in triplicates and incubated in the dark for 2 days. For isolation of MTB, a modified method of racetrack purification, hanging drop and centrifugation technique of MTB were used. The samples were enriched in a modified medium and incubated in a shaker incubator for one week. Pour plate and streak plate methods were done. For characterization, microscopic analysis using optical and scanning electron microscope (SEM) was carried out to observe swimming behaviour of MTB and its magnetosomes if any. The screening of MTB showed that all the samples were Gram-negative but there was no movement of bacteria towards magnetic force and no magnetosomes found. Therefore, it can be assumed that water samples from the two lakes in USM do not have magnetotactic bacteria due to its fastidious growth, hard to isolate in axenic culture and exists in a place with abundant of soluble ferrous form.
format Article
author Minhad, Nurulatika
author_facet Minhad, Nurulatika
author_sort Minhad, Nurulatika
title Screening of magnetotactic bacteria from lakes in Universiti Sains Malaysia, Penang / Nurulatika Minhad
title_short Screening of magnetotactic bacteria from lakes in Universiti Sains Malaysia, Penang / Nurulatika Minhad
title_full Screening of magnetotactic bacteria from lakes in Universiti Sains Malaysia, Penang / Nurulatika Minhad
title_fullStr Screening of magnetotactic bacteria from lakes in Universiti Sains Malaysia, Penang / Nurulatika Minhad
title_full_unstemmed Screening of magnetotactic bacteria from lakes in Universiti Sains Malaysia, Penang / Nurulatika Minhad
title_sort screening of magnetotactic bacteria from lakes in universiti sains malaysia, penang / nurulatika minhad
publisher Universiti Teknologi MARA Cawangan Pahang
publishDate 2020
url http://ir.uitm.edu.my/id/eprint/45934/1/45934.pdf
http://ir.uitm.edu.my/id/eprint/45934/
http://www.gadingst.learningdistance.org/index.php/gadingst/article/view/96
_version_ 1703963398279004160
score 13.211869

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