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Investigation on enzymatic activity of rubber seed as source of plant lipase / Noor Fatiha Ahmad Bahrin, Harumi Veny, and Siti Zainab Che Mat

Rubber seed is a non-edible seed that is abundantly available and considers agricultural wastes. A potential lipase from rubber seed was examined based on the enzymatic activity and its application in the hydrolysis reaction. The enzymatic activity characterization study was determined based on p-ni...

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Main Authors: Ahmad Bahrin, Noor Fatiha, Veny, Harumi, Che Mat, Siti Zainab
Format: Article
Language:English
Published: Universiti Teknologi MARA Shah Alam 2020
Subjects:
Biological laboratories and stations
Online Access:http://ir.uitm.edu.my/id/eprint/43739/1/43739.pdf
http://ir.uitm.edu.my/id/eprint/43739/
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spelling my.uitm.ir.437392021-03-18T04:49:52Z http://ir.uitm.edu.my/id/eprint/43739/ Investigation on enzymatic activity of rubber seed as source of plant lipase / Noor Fatiha Ahmad Bahrin, Harumi Veny, and Siti Zainab Che Mat Ahmad Bahrin, Noor Fatiha Veny, Harumi Che Mat, Siti Zainab Biological laboratories and stations Rubber seed is a non-edible seed that is abundantly available and considers agricultural wastes. A potential lipase from rubber seed was examined based on the enzymatic activity and its application in the hydrolysis reaction. The enzymatic activity characterization study was determined based on p-nitrophenol release in the hydrolysis reaction. The initial evaluation showed that temperature and pH significantly influence the reaction. The optimum condition based on enzymatic activity for rubber seed was found at 30 ℃ and pH 8. The rubber seed lipase extract was then used in enzymatic hydrolysis reactions of rubber seed oil, palm oil, and canola oil. The highest FFA percentage of 63% was found from the rubber seed oil. The results indicate that rubber seed extract has shown its potential enzymatic activity. However, further studies need to be done to apply this rubber seed in various lipase catalysed reactions. Universiti Teknologi MARA Shah Alam 2020 Article PeerReviewed text en http://ir.uitm.edu.my/id/eprint/43739/1/43739.pdf Ahmad Bahrin, Noor Fatiha and Veny, Harumi and Che Mat, Siti Zainab (2020) Investigation on enzymatic activity of rubber seed as source of plant lipase / Noor Fatiha Ahmad Bahrin, Harumi Veny, and Siti Zainab Che Mat. Malaysian Journal of Chemical Engineering and Technology (MJCET), 3 (2). pp. 45-50. ISSN 2682-8588
institution Universiti Teknologi Mara
building Tun Abdul Razak Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Teknologi Mara
content_source UiTM Institutional Repository
url_provider http://ir.uitm.edu.my/
language English
topic Biological laboratories and stations
spellingShingle Biological laboratories and stations
Ahmad Bahrin, Noor Fatiha
Veny, Harumi
Che Mat, Siti Zainab
Investigation on enzymatic activity of rubber seed as source of plant lipase / Noor Fatiha Ahmad Bahrin, Harumi Veny, and Siti Zainab Che Mat
description Rubber seed is a non-edible seed that is abundantly available and considers agricultural wastes. A potential lipase from rubber seed was examined based on the enzymatic activity and its application in the hydrolysis reaction. The enzymatic activity characterization study was determined based on p-nitrophenol release in the hydrolysis reaction. The initial evaluation showed that temperature and pH significantly influence the reaction. The optimum condition based on enzymatic activity for rubber seed was found at 30 ℃ and pH 8. The rubber seed lipase extract was then used in enzymatic hydrolysis reactions of rubber seed oil, palm oil, and canola oil. The highest FFA percentage of 63% was found from the rubber seed oil. The results indicate that rubber seed extract has shown its potential enzymatic activity. However, further studies need to be done to apply this rubber seed in various lipase catalysed reactions.
format Article
author Ahmad Bahrin, Noor Fatiha
Veny, Harumi
Che Mat, Siti Zainab
author_facet Ahmad Bahrin, Noor Fatiha
Veny, Harumi
Che Mat, Siti Zainab
author_sort Ahmad Bahrin, Noor Fatiha
title Investigation on enzymatic activity of rubber seed as source of plant lipase / Noor Fatiha Ahmad Bahrin, Harumi Veny, and Siti Zainab Che Mat
title_short Investigation on enzymatic activity of rubber seed as source of plant lipase / Noor Fatiha Ahmad Bahrin, Harumi Veny, and Siti Zainab Che Mat
title_full Investigation on enzymatic activity of rubber seed as source of plant lipase / Noor Fatiha Ahmad Bahrin, Harumi Veny, and Siti Zainab Che Mat
title_fullStr Investigation on enzymatic activity of rubber seed as source of plant lipase / Noor Fatiha Ahmad Bahrin, Harumi Veny, and Siti Zainab Che Mat
title_full_unstemmed Investigation on enzymatic activity of rubber seed as source of plant lipase / Noor Fatiha Ahmad Bahrin, Harumi Veny, and Siti Zainab Che Mat
title_sort investigation on enzymatic activity of rubber seed as source of plant lipase / noor fatiha ahmad bahrin, harumi veny, and siti zainab che mat
publisher Universiti Teknologi MARA Shah Alam
publishDate 2020
url http://ir.uitm.edu.my/id/eprint/43739/1/43739.pdf
http://ir.uitm.edu.my/id/eprint/43739/
_version_ 1695534704996909056
score 13.211869

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