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Phytochemical screening and bioactivity studies of Momordica charantia / Nur Hazwani Razak

The phytochemical screening and biological activities of Momordica charantia have been studied. Two parts of plant were used in this study such as fruit and seed. The plant has been extracted by using three different polarity of solvents such as n- hexane, chloroform and methanol through the cool ex...

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Main Author: Razak, Nur Hazwani
Format: Student Project
Language:English
Published: 2019
Subjects:
Laboratories. General works
Extraction (Chemistry)
Photochemistry
Online Access:http://ir.uitm.edu.my/id/eprint/42736/1/42736.pdf
http://ir.uitm.edu.my/id/eprint/42736/
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spelling my.uitm.ir.427362021-03-10T02:43:58Z http://ir.uitm.edu.my/id/eprint/42736/ Phytochemical screening and bioactivity studies of Momordica charantia / Nur Hazwani Razak Razak, Nur Hazwani Laboratories. General works Extraction (Chemistry) Photochemistry The phytochemical screening and biological activities of Momordica charantia have been studied. Two parts of plant were used in this study such as fruit and seed. The plant has been extracted by using three different polarity of solvents such as n- hexane, chloroform and methanol through the cool extraction method. The result has shown that the highest percentage yield was methanol fruit extract with 15.29%. The phytochemical screening has revealed there are many secondary metabolites in M. charantia fruit and seed such as alkaloid, flavonoid, saponin, phenol, tannin, terpenoid, steroid and glycoside while for seed part saponin was absence. The solvent system that has been used in Thin layer chromatography (TLC) was the mixture of n-hexane and chloroform with ratio 5:5 and 8:2, to obtain better separation by using vanillin spraying reagent. Antibacterial study has been conducted by using disc diffusion method on Bacillus subtilis, Staphylococcus aureus, Salmonella typhimurium and Escherichia coli. In addition, the result has shown that the highest inhibition zone for fruit and seed was on Staphylococcus aureus in the range of 16 to 17 mm. Meanwhile, the antioxidant study revealed that the fruit and seed of M. charantia do not have antioxidant activity with percentage inhibition less than 50%. 2019 Student Project NonPeerReviewed text en http://ir.uitm.edu.my/id/eprint/42736/1/42736.pdf Razak, Nur Hazwani (2019) Phytochemical screening and bioactivity studies of Momordica charantia / Nur Hazwani Razak. [Student Project] (Unpublished)
institution Universiti Teknologi Mara
building Tun Abdul Razak Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Teknologi Mara
content_source UiTM Institutional Repository
url_provider http://ir.uitm.edu.my/
language English
topic Laboratories. General works
Extraction (Chemistry)
Photochemistry
spellingShingle Laboratories. General works
Extraction (Chemistry)
Photochemistry
Razak, Nur Hazwani
Phytochemical screening and bioactivity studies of Momordica charantia / Nur Hazwani Razak
description The phytochemical screening and biological activities of Momordica charantia have been studied. Two parts of plant were used in this study such as fruit and seed. The plant has been extracted by using three different polarity of solvents such as n- hexane, chloroform and methanol through the cool extraction method. The result has shown that the highest percentage yield was methanol fruit extract with 15.29%. The phytochemical screening has revealed there are many secondary metabolites in M. charantia fruit and seed such as alkaloid, flavonoid, saponin, phenol, tannin, terpenoid, steroid and glycoside while for seed part saponin was absence. The solvent system that has been used in Thin layer chromatography (TLC) was the mixture of n-hexane and chloroform with ratio 5:5 and 8:2, to obtain better separation by using vanillin spraying reagent. Antibacterial study has been conducted by using disc diffusion method on Bacillus subtilis, Staphylococcus aureus, Salmonella typhimurium and Escherichia coli. In addition, the result has shown that the highest inhibition zone for fruit and seed was on Staphylococcus aureus in the range of 16 to 17 mm. Meanwhile, the antioxidant study revealed that the fruit and seed of M. charantia do not have antioxidant activity with percentage inhibition less than 50%.
format Student Project
author Razak, Nur Hazwani
author_facet Razak, Nur Hazwani
author_sort Razak, Nur Hazwani
title Phytochemical screening and bioactivity studies of Momordica charantia / Nur Hazwani Razak
title_short Phytochemical screening and bioactivity studies of Momordica charantia / Nur Hazwani Razak
title_full Phytochemical screening and bioactivity studies of Momordica charantia / Nur Hazwani Razak
title_fullStr Phytochemical screening and bioactivity studies of Momordica charantia / Nur Hazwani Razak
title_full_unstemmed Phytochemical screening and bioactivity studies of Momordica charantia / Nur Hazwani Razak
title_sort phytochemical screening and bioactivity studies of momordica charantia / nur hazwani razak
publishDate 2019
url http://ir.uitm.edu.my/id/eprint/42736/1/42736.pdf
http://ir.uitm.edu.my/id/eprint/42736/
_version_ 1695534632177500160
score 13.211869

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