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Phytochemical screening and bioactivities of Boesenbergia rotunda / Fatin Nurafiqah Ghazali

The qualitative phytochemical screening as well as antioxidant and antibacterial activities of Boesenbergia rotunda (Zingiberaceae) have been investigated. The phytochemicals were extracted by sequential maceration using n-hexane, ethyl acetate and methanol, while phytochemical screening was perform...

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Main Author: Ghazali, Fatin Nurafiqah
Format: Student Project
Language:English
Published: 2019
Subjects:
Laboratories. General works
QD Chemistry
Extraction (Chemistry)
Online Access:http://ir.uitm.edu.my/id/eprint/42669/1/42669.pdf
http://ir.uitm.edu.my/id/eprint/42669/
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spelling my.uitm.ir.426692021-03-09T09:09:27Z http://ir.uitm.edu.my/id/eprint/42669/ Phytochemical screening and bioactivities of Boesenbergia rotunda / Fatin Nurafiqah Ghazali Ghazali, Fatin Nurafiqah Laboratories. General works QD Chemistry Extraction (Chemistry) The qualitative phytochemical screening as well as antioxidant and antibacterial activities of Boesenbergia rotunda (Zingiberaceae) have been investigated. The phytochemicals were extracted by sequential maceration using n-hexane, ethyl acetate and methanol, while phytochemical screening was performed using several chemical tests. 2,2-Diphenyl-1-picryhydrazyl and disc diffusion methods were used to determine antioxidant and antibacterial activities, respectively. Flavonoids, phenols, steroids and terpenes were found to be present in the n¬ hexane, ethyl acetate and methanol extracts. However, alkaloids, saponins, tannins were absent in all extracts. Thin layer chromatography analysis revealed that the best separation of compound for the n-hexane extract was achieved by using n-hexane:diethyl ether (3:2), while better separation of compounds in ethyl acetate extract was accomplished using n-hexane:ethyl acetate (2:3). The best solvent system for methanol extract was n-hexane:ethyl acetate (I:I ). All extracts demonstrated no DPPH radical scavenging activity at concentration of I 000 µg/mL. All extracts showed antibacterial activity towards S. aureus (10.25-I 4.50 mm) and E. coli (9.67-17.00 mm). The methanol extract exhibited the highest inhibitory activity toward S. aureus and E. coli with the inhibition zones diameter were 14.50 and 17.00 mm, respectively. 2019 Student Project NonPeerReviewed text en http://ir.uitm.edu.my/id/eprint/42669/1/42669.pdf Ghazali, Fatin Nurafiqah (2019) Phytochemical screening and bioactivities of Boesenbergia rotunda / Fatin Nurafiqah Ghazali. [Student Project] (Unpublished)
institution Universiti Teknologi Mara
building Tun Abdul Razak Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Teknologi Mara
content_source UiTM Institutional Repository
url_provider http://ir.uitm.edu.my/
language English
topic Laboratories. General works
QD Chemistry
Extraction (Chemistry)
spellingShingle Laboratories. General works
QD Chemistry
Extraction (Chemistry)
Ghazali, Fatin Nurafiqah
Phytochemical screening and bioactivities of Boesenbergia rotunda / Fatin Nurafiqah Ghazali
description The qualitative phytochemical screening as well as antioxidant and antibacterial activities of Boesenbergia rotunda (Zingiberaceae) have been investigated. The phytochemicals were extracted by sequential maceration using n-hexane, ethyl acetate and methanol, while phytochemical screening was performed using several chemical tests. 2,2-Diphenyl-1-picryhydrazyl and disc diffusion methods were used to determine antioxidant and antibacterial activities, respectively. Flavonoids, phenols, steroids and terpenes were found to be present in the n¬ hexane, ethyl acetate and methanol extracts. However, alkaloids, saponins, tannins were absent in all extracts. Thin layer chromatography analysis revealed that the best separation of compound for the n-hexane extract was achieved by using n-hexane:diethyl ether (3:2), while better separation of compounds in ethyl acetate extract was accomplished using n-hexane:ethyl acetate (2:3). The best solvent system for methanol extract was n-hexane:ethyl acetate (I:I ). All extracts demonstrated no DPPH radical scavenging activity at concentration of I 000 µg/mL. All extracts showed antibacterial activity towards S. aureus (10.25-I 4.50 mm) and E. coli (9.67-17.00 mm). The methanol extract exhibited the highest inhibitory activity toward S. aureus and E. coli with the inhibition zones diameter were 14.50 and 17.00 mm, respectively.
format Student Project
author Ghazali, Fatin Nurafiqah
author_facet Ghazali, Fatin Nurafiqah
author_sort Ghazali, Fatin Nurafiqah
title Phytochemical screening and bioactivities of Boesenbergia rotunda / Fatin Nurafiqah Ghazali
title_short Phytochemical screening and bioactivities of Boesenbergia rotunda / Fatin Nurafiqah Ghazali
title_full Phytochemical screening and bioactivities of Boesenbergia rotunda / Fatin Nurafiqah Ghazali
title_fullStr Phytochemical screening and bioactivities of Boesenbergia rotunda / Fatin Nurafiqah Ghazali
title_full_unstemmed Phytochemical screening and bioactivities of Boesenbergia rotunda / Fatin Nurafiqah Ghazali
title_sort phytochemical screening and bioactivities of boesenbergia rotunda / fatin nurafiqah ghazali
publishDate 2019
url http://ir.uitm.edu.my/id/eprint/42669/1/42669.pdf
http://ir.uitm.edu.my/id/eprint/42669/
_version_ 1695534629160747008
score 13.211869

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