Cover Image

Callus induction from the leaves of Ipomoea batatas (sweet potato) / Nor Atikah Ismail

Sweet potato (Ipomoea batatas) is vital crops that are consumed in several countries such as China and Africa. However, only the tubers of sweet potato are use up by many people but the other parts such as leaves and stems are usually discarded. Based on previous study, the leaves of sweet potato pl...

Full description

Saved in:
Bibliographic Details
Main Author: Ismail, Nor Atikah
Format: Student Project
Language:English
Published: 2019
Subjects:
Laboratories. General works
Root and tuber crops
Sweet potatoes
Online Access:http://ir.uitm.edu.my/id/eprint/42205/1/42205.pdf
http://ir.uitm.edu.my/id/eprint/42205/
Tags: Add Tag
No Tags, Be the first to tag this record!
id my.uitm.ir.42205
record_format eprints
spelling my.uitm.ir.422052021-02-23T07:13:57Z http://ir.uitm.edu.my/id/eprint/42205/ Callus induction from the leaves of Ipomoea batatas (sweet potato) / Nor Atikah Ismail Ismail, Nor Atikah Laboratories. General works Root and tuber crops Sweet potatoes Sweet potato (Ipomoea batatas) is vital crops that are consumed in several countries such as China and Africa. However, only the tubers of sweet potato are use up by many people but the other parts such as leaves and stems are usually discarded. Based on previous study, the leaves of sweet potato plant actually contain secondary metabolite. It is proved through a research conducted by Muhamad Ridzuan (2017). The results from his study had revealed the presented of unknown alkaloid in leaves of sweet potato consumed from the wild type plant sample. As published, alkaloid may be used in treatment of disease such as an ovarian cancer. Therefore, the comparison of alkaloid that presented within the callus induced from this study and the wild type plant will be done. Thus, the purpose of this research is to induce the callus from leaves of sweet potato by using different types of hormones such as 2,4-dichlorophenoxyacetic acid (2,4-D), napthaleneacetic acid (NAA) and indole-3-acetic acid (IAA) at different concentrations. Besides from that, the aim of this study is to compare the callus induction from leaves of sweet potato with different types of phytohormone such as, 2,4-dichlorophenoxyacetic acid (2,4-D), napthaleneacetic acid (NAA) and indole-3-acetic acid (IAA) at different concentrations (1.0, 2.0, 3.0, 4.0 and 5.0 mg/L). The explants were cultured in media that contain phytohormone and were observed in 30 days. At the end of research, there can be seen that the best hormone induced callus was IAA, followed by 2,4-D. Unfortunately, there was no callus induced through NAA. Based on concentration, the highest percentage of callus was induced by IAA at 3.0 (13.33%). Meanwhile, the percentage of callus induced by 2,4-D was at 1.0 and 4.0 mg/L concentration (6.67%). For the narrowed down concentration of 2,4-D, the highest percentage of callus was induced at concentration 2.0 mg/L of 2,4-D (20.00%). From the physical appearance, after 4 days it can be seen that the entire callus induced are in white callus. Based on the result, the present of secondary metabolites such as alkaloid can be detected through Thin Layer Chromatography (TLC) from the callus induced. Therefore, the optimization of callus growth by using different media and formulation of concentration phytohormone can be done. In addition, the concentration of the alkaloid can be obtained through High Performance Liquid Chromatography (HPLC). 2019 Student Project NonPeerReviewed text en http://ir.uitm.edu.my/id/eprint/42205/1/42205.pdf Ismail, Nor Atikah (2019) Callus induction from the leaves of Ipomoea batatas (sweet potato) / Nor Atikah Ismail. [Student Project] (Unpublished)
institution Universiti Teknologi Mara
building Tun Abdul Razak Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Teknologi Mara
content_source UiTM Institutional Repository
url_provider http://ir.uitm.edu.my/
language English
topic Laboratories. General works
Root and tuber crops
Sweet potatoes
spellingShingle Laboratories. General works
Root and tuber crops
Sweet potatoes
Ismail, Nor Atikah
Callus induction from the leaves of Ipomoea batatas (sweet potato) / Nor Atikah Ismail
description Sweet potato (Ipomoea batatas) is vital crops that are consumed in several countries such as China and Africa. However, only the tubers of sweet potato are use up by many people but the other parts such as leaves and stems are usually discarded. Based on previous study, the leaves of sweet potato plant actually contain secondary metabolite. It is proved through a research conducted by Muhamad Ridzuan (2017). The results from his study had revealed the presented of unknown alkaloid in leaves of sweet potato consumed from the wild type plant sample. As published, alkaloid may be used in treatment of disease such as an ovarian cancer. Therefore, the comparison of alkaloid that presented within the callus induced from this study and the wild type plant will be done. Thus, the purpose of this research is to induce the callus from leaves of sweet potato by using different types of hormones such as 2,4-dichlorophenoxyacetic acid (2,4-D), napthaleneacetic acid (NAA) and indole-3-acetic acid (IAA) at different concentrations. Besides from that, the aim of this study is to compare the callus induction from leaves of sweet potato with different types of phytohormone such as, 2,4-dichlorophenoxyacetic acid (2,4-D), napthaleneacetic acid (NAA) and indole-3-acetic acid (IAA) at different concentrations (1.0, 2.0, 3.0, 4.0 and 5.0 mg/L). The explants were cultured in media that contain phytohormone and were observed in 30 days. At the end of research, there can be seen that the best hormone induced callus was IAA, followed by 2,4-D. Unfortunately, there was no callus induced through NAA. Based on concentration, the highest percentage of callus was induced by IAA at 3.0 (13.33%). Meanwhile, the percentage of callus induced by 2,4-D was at 1.0 and 4.0 mg/L concentration (6.67%). For the narrowed down concentration of 2,4-D, the highest percentage of callus was induced at concentration 2.0 mg/L of 2,4-D (20.00%). From the physical appearance, after 4 days it can be seen that the entire callus induced are in white callus. Based on the result, the present of secondary metabolites such as alkaloid can be detected through Thin Layer Chromatography (TLC) from the callus induced. Therefore, the optimization of callus growth by using different media and formulation of concentration phytohormone can be done. In addition, the concentration of the alkaloid can be obtained through High Performance Liquid Chromatography (HPLC).
format Student Project
author Ismail, Nor Atikah
author_facet Ismail, Nor Atikah
author_sort Ismail, Nor Atikah
title Callus induction from the leaves of Ipomoea batatas (sweet potato) / Nor Atikah Ismail
title_short Callus induction from the leaves of Ipomoea batatas (sweet potato) / Nor Atikah Ismail
title_full Callus induction from the leaves of Ipomoea batatas (sweet potato) / Nor Atikah Ismail
title_fullStr Callus induction from the leaves of Ipomoea batatas (sweet potato) / Nor Atikah Ismail
title_full_unstemmed Callus induction from the leaves of Ipomoea batatas (sweet potato) / Nor Atikah Ismail
title_sort callus induction from the leaves of ipomoea batatas (sweet potato) / nor atikah ismail
publishDate 2019
url http://ir.uitm.edu.my/id/eprint/42205/1/42205.pdf
http://ir.uitm.edu.my/id/eprint/42205/
_version_ 1692994676801404928
score 13.211869

Similar Items