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Molecular identification and carriage pattern of staphylococcus aureus from nasal isolates among medical laboratory technology students in UiTM Puncak Alam / Iman Abdul Aziz

Staphylococcus aureus (S. aureus) is a normal flora of the nostrils in healthy population and most healthy carriers have S. aureus on their skin without showing any active infection. About 30% of human population carries S. aureus in their nose, which is an important risk for nosocomial and communit...

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Main Author: Abdul Aziz, Iman
Format: Thesis
Language:English
Published: 2016
Subjects:
Medical education. Medical schools. Research
Pharmaceutical technology
Online Access:https://ir.uitm.edu.my/id/eprint/38710/1/38710.pdf
https://ir.uitm.edu.my/id/eprint/38710/
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spelling my.uitm.ir.387102022-07-05T07:41:46Z https://ir.uitm.edu.my/id/eprint/38710/ Molecular identification and carriage pattern of staphylococcus aureus from nasal isolates among medical laboratory technology students in UiTM Puncak Alam / Iman Abdul Aziz Abdul Aziz, Iman Medical education. Medical schools. Research Pharmaceutical technology Staphylococcus aureus (S. aureus) is a normal flora of the nostrils in healthy population and most healthy carriers have S. aureus on their skin without showing any active infection. About 30% of human population carries S. aureus in their nose, which is an important risk for nosocomial and community-acquired infections worldwide. As Medical Laboratory Technology (MLT) students will be interacting and exposed to hospital environments in the future, they may be the potential nasal carriers for spreading the organism to hospital patients. This cross-sectional study involving 74 pre-clinical and 70 clinical students aims to determine the prevalence of S. aureus from nasal isolates among MLT students in UiTM Puncak Alam, classify these students into persistent, intermittent or non-carriers, and evaluate the association between S. aureus nasal colonization and possible risk factors in this population. After signing an informed consent, students completed a Survey Form related to possible risk factors for colonization, and nasal swabs were collected. S. aureus isolated undergo further reconfirmation by the amplification of by real-time Polymerase Chain Reaction (RT-PCR) to determine the presence of nuc gene. To determine the carriage types, each student were subjected to two additional nasal swabs collection, each taken three weeks apart. From 144 nasal swabs collected, 18 (12.5%) were identified as S. aureus carriers. Ten (6.9%) were persistent carriers, 8 (5.6%o) were intermittent carriers and 126 (87.5%) were non-carries. There were no statistical significant association between S. aureus nasal carrier status and gender, race, clinical status, and medical and hygienic factors (p>0.05). In conclusion, only small percentage of MLT students were identified as intermittent or persistent carriers of S. aureus nasal carrier while the majority were non-carrier. 2016 Thesis NonPeerReviewed text en https://ir.uitm.edu.my/id/eprint/38710/1/38710.pdf Molecular identification and carriage pattern of staphylococcus aureus from nasal isolates among medical laboratory technology students in UiTM Puncak Alam / Iman Abdul Aziz. (2016) Degree thesis, thesis, Universiti Teknologi MARA (Kampus Puncak Alam).
institution Universiti Teknologi Mara
building Tun Abdul Razak Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Teknologi Mara
content_source UiTM Institutional Repository
url_provider http://ir.uitm.edu.my/
language English
topic Medical education. Medical schools. Research
Pharmaceutical technology
spellingShingle Medical education. Medical schools. Research
Pharmaceutical technology
Abdul Aziz, Iman
Molecular identification and carriage pattern of staphylococcus aureus from nasal isolates among medical laboratory technology students in UiTM Puncak Alam / Iman Abdul Aziz
description Staphylococcus aureus (S. aureus) is a normal flora of the nostrils in healthy population and most healthy carriers have S. aureus on their skin without showing any active infection. About 30% of human population carries S. aureus in their nose, which is an important risk for nosocomial and community-acquired infections worldwide. As Medical Laboratory Technology (MLT) students will be interacting and exposed to hospital environments in the future, they may be the potential nasal carriers for spreading the organism to hospital patients. This cross-sectional study involving 74 pre-clinical and 70 clinical students aims to determine the prevalence of S. aureus from nasal isolates among MLT students in UiTM Puncak Alam, classify these students into persistent, intermittent or non-carriers, and evaluate the association between S. aureus nasal colonization and possible risk factors in this population. After signing an informed consent, students completed a Survey Form related to possible risk factors for colonization, and nasal swabs were collected. S. aureus isolated undergo further reconfirmation by the amplification of by real-time Polymerase Chain Reaction (RT-PCR) to determine the presence of nuc gene. To determine the carriage types, each student were subjected to two additional nasal swabs collection, each taken three weeks apart. From 144 nasal swabs collected, 18 (12.5%) were identified as S. aureus carriers. Ten (6.9%) were persistent carriers, 8 (5.6%o) were intermittent carriers and 126 (87.5%) were non-carries. There were no statistical significant association between S. aureus nasal carrier status and gender, race, clinical status, and medical and hygienic factors (p>0.05). In conclusion, only small percentage of MLT students were identified as intermittent or persistent carriers of S. aureus nasal carrier while the majority were non-carrier.
format Thesis
author Abdul Aziz, Iman
author_facet Abdul Aziz, Iman
author_sort Abdul Aziz, Iman
title Molecular identification and carriage pattern of staphylococcus aureus from nasal isolates among medical laboratory technology students in UiTM Puncak Alam / Iman Abdul Aziz
title_short Molecular identification and carriage pattern of staphylococcus aureus from nasal isolates among medical laboratory technology students in UiTM Puncak Alam / Iman Abdul Aziz
title_full Molecular identification and carriage pattern of staphylococcus aureus from nasal isolates among medical laboratory technology students in UiTM Puncak Alam / Iman Abdul Aziz
title_fullStr Molecular identification and carriage pattern of staphylococcus aureus from nasal isolates among medical laboratory technology students in UiTM Puncak Alam / Iman Abdul Aziz
title_full_unstemmed Molecular identification and carriage pattern of staphylococcus aureus from nasal isolates among medical laboratory technology students in UiTM Puncak Alam / Iman Abdul Aziz
title_sort molecular identification and carriage pattern of staphylococcus aureus from nasal isolates among medical laboratory technology students in uitm puncak alam / iman abdul aziz
publishDate 2016
url https://ir.uitm.edu.my/id/eprint/38710/1/38710.pdf
https://ir.uitm.edu.my/id/eprint/38710/
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score 13.211869

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