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Cytotoxicity and genotoxicity studies of selected medicinal mushrooms extracts using Chinese hamster lung fibroblast cell line (V79) / Nur Shahirah Nasir

Medicinal mushrooms are well-known for nutritional benefits. However, it is necessary to evaluate the toxicity of medicinal mushrooms. The aim of this study was to identify the potential toxicity of Agaricus blazei, Grifola frondosa and Hericium erinaceus. In this study, screening of phytochemical c...

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Main Author: Nasir, Nur Shahirah
Format: Thesis
Language:English
Published: 2017
Subjects:
Individual drugs and other agents
Medicinal plants (Culture only)
Online Access:https://ir.uitm.edu.my/id/eprint/37323/1/37323.pdf
https://ir.uitm.edu.my/id/eprint/37323/
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spelling my.uitm.ir.373232022-11-01T08:24:54Z https://ir.uitm.edu.my/id/eprint/37323/ Cytotoxicity and genotoxicity studies of selected medicinal mushrooms extracts using Chinese hamster lung fibroblast cell line (V79) / Nur Shahirah Nasir Nasir, Nur Shahirah Individual drugs and other agents Medicinal plants (Culture only) Medicinal mushrooms are well-known for nutritional benefits. However, it is necessary to evaluate the toxicity of medicinal mushrooms. The aim of this study was to identify the potential toxicity of Agaricus blazei, Grifola frondosa and Hericium erinaceus. In this study, screening of phytochemical compound, evaluating the cytotoxicity effect (MTT assay), determining the mode of cell death (Annexin V), observing the morphological changes of cell and analysing the genotoxicity (Comet Asssay) were done to assess the potential toxicity. All toxicity tests were evaluated against Chinese hamster lung fibroblast cell line (V79) using methanol and aqueous of the extracts at 0.125 mg/ml, 0.25 mg/ml, 0.5 mg/ml, 1 mg/ml and 2 mg/ml. Phytochemical screening showed the extracts contain abundantly of alkaloid. The MTT assay demonstrated that all extracts showed low cytotoxicity effect at 2 mg/ml. Only aqueous extract of A. blazei displayed the highest cytotoxicity effect at 1.7 mg/ml (IC50). The Mode of Cell Death for viable V79 cells for A. blazei was higher at 2 mg/ml (93.9% ±0.721) compared to apoptosis and necrosis using Annexin V. Cell morphology did not show any sign of apoptotic and cell injury at 1 mg/ml and 2 mg/ml of mushroom extract during 24 hours treatment. Lastly, genotoxicity activity for A. blazei, G. frondosa and H. erinaceus in V79 cell results revealed that DNA damages were detected at 1 mg/ml and 2 mg/ml. In conclusion, these three selected medicinal mushrooms had caused toxicity to normal cells as demonstrated by V79 cell line at 1 to 2 mg/ml. 2017 Thesis NonPeerReviewed text en https://ir.uitm.edu.my/id/eprint/37323/1/37323.pdf Cytotoxicity and genotoxicity studies of selected medicinal mushrooms extracts using Chinese hamster lung fibroblast cell line (V79) / Nur Shahirah Nasir. (2017) Masters thesis, thesis, Universiti Teknologi MARA (UiTM).
institution Universiti Teknologi Mara
building Tun Abdul Razak Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Teknologi Mara
content_source UiTM Institutional Repository
url_provider http://ir.uitm.edu.my/
language English
topic Individual drugs and other agents
Medicinal plants (Culture only)
spellingShingle Individual drugs and other agents
Medicinal plants (Culture only)
Nasir, Nur Shahirah
Cytotoxicity and genotoxicity studies of selected medicinal mushrooms extracts using Chinese hamster lung fibroblast cell line (V79) / Nur Shahirah Nasir
description Medicinal mushrooms are well-known for nutritional benefits. However, it is necessary to evaluate the toxicity of medicinal mushrooms. The aim of this study was to identify the potential toxicity of Agaricus blazei, Grifola frondosa and Hericium erinaceus. In this study, screening of phytochemical compound, evaluating the cytotoxicity effect (MTT assay), determining the mode of cell death (Annexin V), observing the morphological changes of cell and analysing the genotoxicity (Comet Asssay) were done to assess the potential toxicity. All toxicity tests were evaluated against Chinese hamster lung fibroblast cell line (V79) using methanol and aqueous of the extracts at 0.125 mg/ml, 0.25 mg/ml, 0.5 mg/ml, 1 mg/ml and 2 mg/ml. Phytochemical screening showed the extracts contain abundantly of alkaloid. The MTT assay demonstrated that all extracts showed low cytotoxicity effect at 2 mg/ml. Only aqueous extract of A. blazei displayed the highest cytotoxicity effect at 1.7 mg/ml (IC50). The Mode of Cell Death for viable V79 cells for A. blazei was higher at 2 mg/ml (93.9% ±0.721) compared to apoptosis and necrosis using Annexin V. Cell morphology did not show any sign of apoptotic and cell injury at 1 mg/ml and 2 mg/ml of mushroom extract during 24 hours treatment. Lastly, genotoxicity activity for A. blazei, G. frondosa and H. erinaceus in V79 cell results revealed that DNA damages were detected at 1 mg/ml and 2 mg/ml. In conclusion, these three selected medicinal mushrooms had caused toxicity to normal cells as demonstrated by V79 cell line at 1 to 2 mg/ml.
format Thesis
author Nasir, Nur Shahirah
author_facet Nasir, Nur Shahirah
author_sort Nasir, Nur Shahirah
title Cytotoxicity and genotoxicity studies of selected medicinal mushrooms extracts using Chinese hamster lung fibroblast cell line (V79) / Nur Shahirah Nasir
title_short Cytotoxicity and genotoxicity studies of selected medicinal mushrooms extracts using Chinese hamster lung fibroblast cell line (V79) / Nur Shahirah Nasir
title_full Cytotoxicity and genotoxicity studies of selected medicinal mushrooms extracts using Chinese hamster lung fibroblast cell line (V79) / Nur Shahirah Nasir
title_fullStr Cytotoxicity and genotoxicity studies of selected medicinal mushrooms extracts using Chinese hamster lung fibroblast cell line (V79) / Nur Shahirah Nasir
title_full_unstemmed Cytotoxicity and genotoxicity studies of selected medicinal mushrooms extracts using Chinese hamster lung fibroblast cell line (V79) / Nur Shahirah Nasir
title_sort cytotoxicity and genotoxicity studies of selected medicinal mushrooms extracts using chinese hamster lung fibroblast cell line (v79) / nur shahirah nasir
publishDate 2017
url https://ir.uitm.edu.my/id/eprint/37323/1/37323.pdf
https://ir.uitm.edu.my/id/eprint/37323/
_version_ 1748705973499854848
score 13.211869

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