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Antifungal study of lawsonia inermis and tamarindus indica leaves on skin dermatopythes fungus (Trichophyton rubrum) / Monisha Adam Gajen

Ringworm skin disorder or dermatophytosis is a common skin problem that affect humans and animals. In present times, there have been various antifungal treatments that have been used in treating this problem. However, Trichophyton rubrum fungi has developed a resistance towards most antifungal drugs...

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Main Author: Adam Gajen, Monisha
Format: Thesis
Language:English
Published: 2020
Subjects:
Fungal antigens
Skin manifestations of systemic disease
Online Access:http://ir.uitm.edu.my/id/eprint/36856/1/36856.pdf
http://ir.uitm.edu.my/id/eprint/36856/
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spelling my.uitm.ir.368562020-11-24T00:39:54Z http://ir.uitm.edu.my/id/eprint/36856/ Antifungal study of lawsonia inermis and tamarindus indica leaves on skin dermatopythes fungus (Trichophyton rubrum) / Monisha Adam Gajen Adam Gajen, Monisha Fungal antigens Skin manifestations of systemic disease Ringworm skin disorder or dermatophytosis is a common skin problem that affect humans and animals. In present times, there have been various antifungal treatments that have been used in treating this problem. However, Trichophyton rubrum fungi has developed a resistance towards most antifungal drugs that are expensive and may cause side effects in the long run. Therefore, this study focuses on combating dermatophytosis which is caused by fungi known as Trichophyton rubrum by the usage of medical plant which are Lawsonia inermis (Henna) and Tamarindus indica (Tamarind) leaves. Both of these are believed to have potential in antifungal effects, economical and safer to be commercialized as an antifungal agent. The objectives of this study are to compare both L. inermis and T. indica effectiveness on different concentrations such as 5 mg/mL, 2.5 mg/mL, 1.25 mg/mL, 0.625 mg/mL and 0.3125 mg/mL against T. rubrum as well as to determine the toxicity of both leaves extract. Besides, the method applied in this study for plant extraction is by using maceration and soaking method using 80% ethanol. The in-vitro antifungal assay was carried out by disc diffusion method. Result obtained indicated that Henna (20.80±6.77 mm) showed better antifungal reaction compared to Tamarind (15.07±5.09 mm). Whereas for positive control Itraconazole (23.33±7.90 mm) showed greater antifungal reaction compared to Fluconazole (17.60±8.85 mm). One major trend found in this study was the higher the concentrations (mg/mL) applied for all four groups of Henna, Tamarind, Fluconazole and Itraconazole, the higher the diameter zone inhibition (mm). Furthermore, toxicity assay result have showed least toxic effect on Tamarind at 43.33% mortality of nauplii followed by Henna at 56.67% mortality of nauplii. However, the mortality percentage were found to be increased as concentration increased for all treatments. In conclusion, it is believed that L. inermis and T. indica crude extract that have been tested have a great potential in antifungal effect against dermophytes of T. rubrum and it could be taken as measure in developing new antifungal treatment in order to fight ringworm skin disorder. 2020-11-13 Thesis NonPeerReviewed text en http://ir.uitm.edu.my/id/eprint/36856/1/36856.pdf Adam Gajen, Monisha (2020) Antifungal study of lawsonia inermis and tamarindus indica leaves on skin dermatopythes fungus (Trichophyton rubrum) / Monisha Adam Gajen. Degree thesis, Universiti Teknologi Mara Perlis.
institution Universiti Teknologi Mara
building Tun Abdul Razak Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Teknologi Mara
content_source UiTM Institutional Repository
url_provider http://ir.uitm.edu.my/
language English
topic Fungal antigens
Skin manifestations of systemic disease
spellingShingle Fungal antigens
Skin manifestations of systemic disease
Adam Gajen, Monisha
Antifungal study of lawsonia inermis and tamarindus indica leaves on skin dermatopythes fungus (Trichophyton rubrum) / Monisha Adam Gajen
description Ringworm skin disorder or dermatophytosis is a common skin problem that affect humans and animals. In present times, there have been various antifungal treatments that have been used in treating this problem. However, Trichophyton rubrum fungi has developed a resistance towards most antifungal drugs that are expensive and may cause side effects in the long run. Therefore, this study focuses on combating dermatophytosis which is caused by fungi known as Trichophyton rubrum by the usage of medical plant which are Lawsonia inermis (Henna) and Tamarindus indica (Tamarind) leaves. Both of these are believed to have potential in antifungal effects, economical and safer to be commercialized as an antifungal agent. The objectives of this study are to compare both L. inermis and T. indica effectiveness on different concentrations such as 5 mg/mL, 2.5 mg/mL, 1.25 mg/mL, 0.625 mg/mL and 0.3125 mg/mL against T. rubrum as well as to determine the toxicity of both leaves extract. Besides, the method applied in this study for plant extraction is by using maceration and soaking method using 80% ethanol. The in-vitro antifungal assay was carried out by disc diffusion method. Result obtained indicated that Henna (20.80±6.77 mm) showed better antifungal reaction compared to Tamarind (15.07±5.09 mm). Whereas for positive control Itraconazole (23.33±7.90 mm) showed greater antifungal reaction compared to Fluconazole (17.60±8.85 mm). One major trend found in this study was the higher the concentrations (mg/mL) applied for all four groups of Henna, Tamarind, Fluconazole and Itraconazole, the higher the diameter zone inhibition (mm). Furthermore, toxicity assay result have showed least toxic effect on Tamarind at 43.33% mortality of nauplii followed by Henna at 56.67% mortality of nauplii. However, the mortality percentage were found to be increased as concentration increased for all treatments. In conclusion, it is believed that L. inermis and T. indica crude extract that have been tested have a great potential in antifungal effect against dermophytes of T. rubrum and it could be taken as measure in developing new antifungal treatment in order to fight ringworm skin disorder.
format Thesis
author Adam Gajen, Monisha
author_facet Adam Gajen, Monisha
author_sort Adam Gajen, Monisha
title Antifungal study of lawsonia inermis and tamarindus indica leaves on skin dermatopythes fungus (Trichophyton rubrum) / Monisha Adam Gajen
title_short Antifungal study of lawsonia inermis and tamarindus indica leaves on skin dermatopythes fungus (Trichophyton rubrum) / Monisha Adam Gajen
title_full Antifungal study of lawsonia inermis and tamarindus indica leaves on skin dermatopythes fungus (Trichophyton rubrum) / Monisha Adam Gajen
title_fullStr Antifungal study of lawsonia inermis and tamarindus indica leaves on skin dermatopythes fungus (Trichophyton rubrum) / Monisha Adam Gajen
title_full_unstemmed Antifungal study of lawsonia inermis and tamarindus indica leaves on skin dermatopythes fungus (Trichophyton rubrum) / Monisha Adam Gajen
title_sort antifungal study of lawsonia inermis and tamarindus indica leaves on skin dermatopythes fungus (trichophyton rubrum) / monisha adam gajen
publishDate 2020
url http://ir.uitm.edu.my/id/eprint/36856/1/36856.pdf
http://ir.uitm.edu.my/id/eprint/36856/
_version_ 1685651459331325952
score 13.211869

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