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Determination of phenol in water sample using high performance liquid chromatography / Nazurah Haron

Phenol as in water is harmful to living organism yet have a toxicity effect for a long-term exposure. In this research, the analysis was achieved on a C18 column coupled with UV-vis detector (HPLC-UV) for determination of phenol in environmental water while by using solid phase extraction (SPE) as s...

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Main Author: Haron, Nazurah
Format: Student Project
Language:English
Published: 2017
Subjects:
Laboratories. General works
Extraction (Chemistry)
Online Access:http://ir.uitm.edu.my/id/eprint/35055/1/35055.pdf
http://ir.uitm.edu.my/id/eprint/35055/
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spelling my.uitm.ir.350552020-10-12T04:18:51Z http://ir.uitm.edu.my/id/eprint/35055/ Determination of phenol in water sample using high performance liquid chromatography / Nazurah Haron Haron, Nazurah Laboratories. General works Extraction (Chemistry) Phenol as in water is harmful to living organism yet have a toxicity effect for a long-term exposure. In this research, the analysis was achieved on a C18 column coupled with UV-vis detector (HPLC-UV) for determination of phenol in environmental water while by using solid phase extraction (SPE) as sample preparation technique. The optimized variable involved in SPE and HPLC were as follow: 3 mL was used for load sample volume in SPE, 45 ± 3 drops min-1 was used for sample drop-rate in SPE and 0.35 mL min-1 as flow-rate on eluent in HPLC. The analytical method was validated based on the following parameter: precision, linear range, limit of detection (LOD), limit of quantitation (LOQ) and bias/recovery. A good linear correlation coefficient with R2 = 0.9996 was observed over the range of 0.55 - 30.0 µg mL-1. Both repeatability and reproducibility (RSD, %) were 0.215 and 1.490, respectively. The limit of detection was calculated to be 0.212 µg mL-1, while the limit of quantitation value of the validated method was measured to be 0.642 µg mL-1. Good recoveries were between 81 - 120%. The proposed method was found to be suitable and precise for the determination of phenol in environmental water by using HPLC. 2017 Student Project NonPeerReviewed text en http://ir.uitm.edu.my/id/eprint/35055/1/35055.pdf Haron, Nazurah (2017) Determination of phenol in water sample using high performance liquid chromatography / Nazurah Haron. [Student Project] (Unpublished)
institution Universiti Teknologi Mara
building Tun Abdul Razak Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Teknologi Mara
content_source UiTM Institutional Repository
url_provider http://ir.uitm.edu.my/
language English
topic Laboratories. General works
Extraction (Chemistry)
spellingShingle Laboratories. General works
Extraction (Chemistry)
Haron, Nazurah
Determination of phenol in water sample using high performance liquid chromatography / Nazurah Haron
description Phenol as in water is harmful to living organism yet have a toxicity effect for a long-term exposure. In this research, the analysis was achieved on a C18 column coupled with UV-vis detector (HPLC-UV) for determination of phenol in environmental water while by using solid phase extraction (SPE) as sample preparation technique. The optimized variable involved in SPE and HPLC were as follow: 3 mL was used for load sample volume in SPE, 45 ± 3 drops min-1 was used for sample drop-rate in SPE and 0.35 mL min-1 as flow-rate on eluent in HPLC. The analytical method was validated based on the following parameter: precision, linear range, limit of detection (LOD), limit of quantitation (LOQ) and bias/recovery. A good linear correlation coefficient with R2 = 0.9996 was observed over the range of 0.55 - 30.0 µg mL-1. Both repeatability and reproducibility (RSD, %) were 0.215 and 1.490, respectively. The limit of detection was calculated to be 0.212 µg mL-1, while the limit of quantitation value of the validated method was measured to be 0.642 µg mL-1. Good recoveries were between 81 - 120%. The proposed method was found to be suitable and precise for the determination of phenol in environmental water by using HPLC.
format Student Project
author Haron, Nazurah
author_facet Haron, Nazurah
author_sort Haron, Nazurah
title Determination of phenol in water sample using high performance liquid chromatography / Nazurah Haron
title_short Determination of phenol in water sample using high performance liquid chromatography / Nazurah Haron
title_full Determination of phenol in water sample using high performance liquid chromatography / Nazurah Haron
title_fullStr Determination of phenol in water sample using high performance liquid chromatography / Nazurah Haron
title_full_unstemmed Determination of phenol in water sample using high performance liquid chromatography / Nazurah Haron
title_sort determination of phenol in water sample using high performance liquid chromatography / nazurah haron
publishDate 2017
url http://ir.uitm.edu.my/id/eprint/35055/1/35055.pdf
http://ir.uitm.edu.my/id/eprint/35055/
_version_ 1685651259142438912
score 13.211869

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