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Respond of seed culture of Ricinus communis toward different hormone treatment in vitro / Muhamad Saifulnizam Tahirudin

This research work aimed to highlight on the effect of various plant growth regulators namely 1-Naphthaleneacetic Acid (NAA) and 6-Benzylaminopurine (BAP) on inducing response toward Ricinus communis in vitro tissue culture. The response observe may be shoot formation, root formation or callus forma...

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Main Author: Tahirudin, Muhamad Saifulnizam
Format: Student Project
Language:English
Published: 2016
Subjects:
Laboratories. General works
Growth
Growth. Development. Including pattern formation
Online Access:http://ir.uitm.edu.my/id/eprint/34502/1/34502.pdf
http://ir.uitm.edu.my/id/eprint/34502/
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spelling my.uitm.ir.345022020-09-28T04:11:50Z http://ir.uitm.edu.my/id/eprint/34502/ Respond of seed culture of Ricinus communis toward different hormone treatment in vitro / Muhamad Saifulnizam Tahirudin Tahirudin, Muhamad Saifulnizam Laboratories. General works Growth Growth. Development. Including pattern formation This research work aimed to highlight on the effect of various plant growth regulators namely 1-Naphthaleneacetic Acid (NAA) and 6-Benzylaminopurine (BAP) on inducing response toward Ricinus communis in vitro tissue culture. The response observe may be shoot formation, root formation or callus formation that were initiated from seed explant of wild Ricinus communis plant located around Parit Tinggi, Kuala Pilah. Different combinations of hormone treatment were added into MS medium containing sucrose, macronutrient and micronutrient. Combination of auxin, NAA and cytokinin, BAP gave different response toward the seed explant. After two weeks of inoculation in sterile environment, the response was observed from the seed explant. About 25% of the incubated seed germinated in the MS basal medium. As a result, 0.5, 1.5 and 2.0 mg/1 of NAA combined with 2.5mg/l BAP gave response where callus formation can be observed in this hormone treatment. Shoot formation was observed at 1.5 mg/I NAA and 2.5 BAP and at 1.0, 1.5, 2.0 and 2.5 mg/I NAA combined with 2.0 mg/1 BAP, there was an enlargement of seed explant where the seed enlarged from average of 0.5 cm to 2 cm. In conclusion, different combination of auxin and cytokinin give rise to different response toward the seed explant of Ricinus communis. 2016 Student Project NonPeerReviewed text en http://ir.uitm.edu.my/id/eprint/34502/1/34502.pdf Tahirudin, Muhamad Saifulnizam (2016) Respond of seed culture of Ricinus communis toward different hormone treatment in vitro / Muhamad Saifulnizam Tahirudin. [Student Project] (Unpublished)
institution Universiti Teknologi Mara
building Tun Abdul Razak Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Teknologi Mara
content_source UiTM Institutional Repository
url_provider http://ir.uitm.edu.my/
language English
topic Laboratories. General works
Growth
Growth. Development. Including pattern formation
spellingShingle Laboratories. General works
Growth
Growth. Development. Including pattern formation
Tahirudin, Muhamad Saifulnizam
Respond of seed culture of Ricinus communis toward different hormone treatment in vitro / Muhamad Saifulnizam Tahirudin
description This research work aimed to highlight on the effect of various plant growth regulators namely 1-Naphthaleneacetic Acid (NAA) and 6-Benzylaminopurine (BAP) on inducing response toward Ricinus communis in vitro tissue culture. The response observe may be shoot formation, root formation or callus formation that were initiated from seed explant of wild Ricinus communis plant located around Parit Tinggi, Kuala Pilah. Different combinations of hormone treatment were added into MS medium containing sucrose, macronutrient and micronutrient. Combination of auxin, NAA and cytokinin, BAP gave different response toward the seed explant. After two weeks of inoculation in sterile environment, the response was observed from the seed explant. About 25% of the incubated seed germinated in the MS basal medium. As a result, 0.5, 1.5 and 2.0 mg/1 of NAA combined with 2.5mg/l BAP gave response where callus formation can be observed in this hormone treatment. Shoot formation was observed at 1.5 mg/I NAA and 2.5 BAP and at 1.0, 1.5, 2.0 and 2.5 mg/I NAA combined with 2.0 mg/1 BAP, there was an enlargement of seed explant where the seed enlarged from average of 0.5 cm to 2 cm. In conclusion, different combination of auxin and cytokinin give rise to different response toward the seed explant of Ricinus communis.
format Student Project
author Tahirudin, Muhamad Saifulnizam
author_facet Tahirudin, Muhamad Saifulnizam
author_sort Tahirudin, Muhamad Saifulnizam
title Respond of seed culture of Ricinus communis toward different hormone treatment in vitro / Muhamad Saifulnizam Tahirudin
title_short Respond of seed culture of Ricinus communis toward different hormone treatment in vitro / Muhamad Saifulnizam Tahirudin
title_full Respond of seed culture of Ricinus communis toward different hormone treatment in vitro / Muhamad Saifulnizam Tahirudin
title_fullStr Respond of seed culture of Ricinus communis toward different hormone treatment in vitro / Muhamad Saifulnizam Tahirudin
title_full_unstemmed Respond of seed culture of Ricinus communis toward different hormone treatment in vitro / Muhamad Saifulnizam Tahirudin
title_sort respond of seed culture of ricinus communis toward different hormone treatment in vitro / muhamad saifulnizam tahirudin
publishDate 2016
url http://ir.uitm.edu.my/id/eprint/34502/1/34502.pdf
http://ir.uitm.edu.my/id/eprint/34502/
_version_ 1685651203984195584
score 13.211869

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