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The effect of nanosilver on sterilization of curculigo latifolia dryand shoot cultures / Zahirah Mohd Zain

Curculigo latifolia Dryand or Lemba is a herb that usually found in the forest that possessed unique sweetening properties that obtained by consuming its fruit. This unique properties can be exhibited if the fruit was consumed with foods that contained acidic properties. Growth of C.latifolia is ver...

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Main Author: Mohd Zain, Zahirah
Format: Student Project
Language:English
Published: 2018
Subjects:
Laboratories. General works
Acids and bases
Online Access:http://ir.uitm.edu.my/id/eprint/34414/1/34414.pdf
http://ir.uitm.edu.my/id/eprint/34414/
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spelling my.uitm.ir.344142020-09-14T09:33:39Z http://ir.uitm.edu.my/id/eprint/34414/ The effect of nanosilver on sterilization of curculigo latifolia dryand shoot cultures / Zahirah Mohd Zain Mohd Zain, Zahirah Laboratories. General works Acids and bases Curculigo latifolia Dryand or Lemba is a herb that usually found in the forest that possessed unique sweetening properties that obtained by consuming its fruit. This unique properties can be exhibited if the fruit was consumed with foods that contained acidic properties. Growth of C.latifolia is very low in the forest and even with micro-propagation techniques as it is easily contaminated and undergo browning. This is proven by many previous study on C.latifolia that attempt on reducing contamination or the study is limited by contamination. The shoot of C.latifolia was surface sterilized with 3 drops of Tween20 for 35 minutes, 30% and 20% Clorox for 15 minutes for both concentration, and 70% alcohol for 90 seconds. After surface sterilization, the shoot explants was then immersed in nanosilver for 5 different concentration (20, 40, 60, 80 and 100ppm) with combination of 3 time of exposure (30, 60 and 90 min) to determine which combination is most effective in eliminating or reducing contamination. Explants sterilized with 100ppm of nanosilver with 60 min time of exposure was the best treatment in reducing contaminants. This treatment had the longest means day of survival which is 24.70 days. Explants undergo necrosis suggesting that the cultures needed to be subcultured within range of 2 weeks to avoid depletion of nutrients. 2018 Student Project NonPeerReviewed text en http://ir.uitm.edu.my/id/eprint/34414/1/34414.pdf Mohd Zain, Zahirah (2018) The effect of nanosilver on sterilization of curculigo latifolia dryand shoot cultures / Zahirah Mohd Zain. [Student Project] (Unpublished)
institution Universiti Teknologi Mara
building Tun Abdul Razak Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Teknologi Mara
content_source UiTM Institutional Repository
url_provider http://ir.uitm.edu.my/
language English
topic Laboratories. General works
Acids and bases
spellingShingle Laboratories. General works
Acids and bases
Mohd Zain, Zahirah
The effect of nanosilver on sterilization of curculigo latifolia dryand shoot cultures / Zahirah Mohd Zain
description Curculigo latifolia Dryand or Lemba is a herb that usually found in the forest that possessed unique sweetening properties that obtained by consuming its fruit. This unique properties can be exhibited if the fruit was consumed with foods that contained acidic properties. Growth of C.latifolia is very low in the forest and even with micro-propagation techniques as it is easily contaminated and undergo browning. This is proven by many previous study on C.latifolia that attempt on reducing contamination or the study is limited by contamination. The shoot of C.latifolia was surface sterilized with 3 drops of Tween20 for 35 minutes, 30% and 20% Clorox for 15 minutes for both concentration, and 70% alcohol for 90 seconds. After surface sterilization, the shoot explants was then immersed in nanosilver for 5 different concentration (20, 40, 60, 80 and 100ppm) with combination of 3 time of exposure (30, 60 and 90 min) to determine which combination is most effective in eliminating or reducing contamination. Explants sterilized with 100ppm of nanosilver with 60 min time of exposure was the best treatment in reducing contaminants. This treatment had the longest means day of survival which is 24.70 days. Explants undergo necrosis suggesting that the cultures needed to be subcultured within range of 2 weeks to avoid depletion of nutrients.
format Student Project
author Mohd Zain, Zahirah
author_facet Mohd Zain, Zahirah
author_sort Mohd Zain, Zahirah
title The effect of nanosilver on sterilization of curculigo latifolia dryand shoot cultures / Zahirah Mohd Zain
title_short The effect of nanosilver on sterilization of curculigo latifolia dryand shoot cultures / Zahirah Mohd Zain
title_full The effect of nanosilver on sterilization of curculigo latifolia dryand shoot cultures / Zahirah Mohd Zain
title_fullStr The effect of nanosilver on sterilization of curculigo latifolia dryand shoot cultures / Zahirah Mohd Zain
title_full_unstemmed The effect of nanosilver on sterilization of curculigo latifolia dryand shoot cultures / Zahirah Mohd Zain
title_sort effect of nanosilver on sterilization of curculigo latifolia dryand shoot cultures / zahirah mohd zain
publishDate 2018
url http://ir.uitm.edu.my/id/eprint/34414/1/34414.pdf
http://ir.uitm.edu.my/id/eprint/34414/
_version_ 1685651191708516352
score 13.211869

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