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Antibacterial activity on stem and leaf of neptunia natans extract / Nur Nazihani Muhamad Nazir

The leaf and stem of methanolic extraction of Neptunia natans were investigated for their antibacterial activity against Salmonella spp. (Gram negative bacteria) and Staphylococcus spp. (Gram positive bacteria). The tests were conducted in vitro and two different methods, which were disc diffusion a...

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Main Author: Muhamad Nazir, Nur Nazihani
Format: Student Project
Language:English
Published: 2018
Subjects:
Laboratories. General works
Extraction (Chemistry)
Bacteria
Online Access:http://ir.uitm.edu.my/id/eprint/34325/1/34325.pdf
http://ir.uitm.edu.my/id/eprint/34325/
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spelling my.uitm.ir.343252020-09-08T08:46:03Z http://ir.uitm.edu.my/id/eprint/34325/ Antibacterial activity on stem and leaf of neptunia natans extract / Nur Nazihani Muhamad Nazir Muhamad Nazir, Nur Nazihani Laboratories. General works Extraction (Chemistry) Bacteria The leaf and stem of methanolic extraction of Neptunia natans were investigated for their antibacterial activity against Salmonella spp. (Gram negative bacteria) and Staphylococcus spp. (Gram positive bacteria). The tests were conducted in vitro and two different methods, which were disc diffusion and agar well diffusion method, were used to evaluate this study. Both of the methods have almost similar technique but it differs in term of sensitivity towards the metabolites compound in the plant. Three different dose of concentration were used to measure the antibacterial activity against selected bacteria which were 500mg/ml, 700mg/ml and 1000mg/ml. The inhibition zones for both samples of leaf and stem of Neptunia natans were measured and compared. Streptomycin and dimethyl sulfoxide (DMSO) act as positive and negative control respectively. Leaves extract from methanol demonstrate a relatively higher inhibition zones of compared to stem. Leaves exhibit 15.07±2.30mm zone of inhibition against Staphylococcus spp. using agar well diffusion method meanwhile, stem showed 11.50±1.06mm zones of inhibition against the same bacteria by using the same method. However, the value of significance difference in statistical analysis were all p>0.05. This indicate that the result showed no significance difference between all the three different concentrations of the extracts used. The minimum inhibitory concentration (MIC) for leaves against both of the bacteria was 62.5mg/ml. Concurrently, 125mg/ml were regarded as MIC for stem methanolic extract. Thus, it can be concluded that the leaves have a better potential to become an antibacterial agent compared to the stem. 2018 Student Project NonPeerReviewed text en http://ir.uitm.edu.my/id/eprint/34325/1/34325.pdf Muhamad Nazir, Nur Nazihani (2018) Antibacterial activity on stem and leaf of neptunia natans extract / Nur Nazihani Muhamad Nazir. [Student Project] (Unpublished)
institution Universiti Teknologi Mara
building Tun Abdul Razak Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Teknologi Mara
content_source UiTM Institutional Repository
url_provider http://ir.uitm.edu.my/
language English
topic Laboratories. General works
Extraction (Chemistry)
Bacteria
spellingShingle Laboratories. General works
Extraction (Chemistry)
Bacteria
Muhamad Nazir, Nur Nazihani
Antibacterial activity on stem and leaf of neptunia natans extract / Nur Nazihani Muhamad Nazir
description The leaf and stem of methanolic extraction of Neptunia natans were investigated for their antibacterial activity against Salmonella spp. (Gram negative bacteria) and Staphylococcus spp. (Gram positive bacteria). The tests were conducted in vitro and two different methods, which were disc diffusion and agar well diffusion method, were used to evaluate this study. Both of the methods have almost similar technique but it differs in term of sensitivity towards the metabolites compound in the plant. Three different dose of concentration were used to measure the antibacterial activity against selected bacteria which were 500mg/ml, 700mg/ml and 1000mg/ml. The inhibition zones for both samples of leaf and stem of Neptunia natans were measured and compared. Streptomycin and dimethyl sulfoxide (DMSO) act as positive and negative control respectively. Leaves extract from methanol demonstrate a relatively higher inhibition zones of compared to stem. Leaves exhibit 15.07±2.30mm zone of inhibition against Staphylococcus spp. using agar well diffusion method meanwhile, stem showed 11.50±1.06mm zones of inhibition against the same bacteria by using the same method. However, the value of significance difference in statistical analysis were all p>0.05. This indicate that the result showed no significance difference between all the three different concentrations of the extracts used. The minimum inhibitory concentration (MIC) for leaves against both of the bacteria was 62.5mg/ml. Concurrently, 125mg/ml were regarded as MIC for stem methanolic extract. Thus, it can be concluded that the leaves have a better potential to become an antibacterial agent compared to the stem.
format Student Project
author Muhamad Nazir, Nur Nazihani
author_facet Muhamad Nazir, Nur Nazihani
author_sort Muhamad Nazir, Nur Nazihani
title Antibacterial activity on stem and leaf of neptunia natans extract / Nur Nazihani Muhamad Nazir
title_short Antibacterial activity on stem and leaf of neptunia natans extract / Nur Nazihani Muhamad Nazir
title_full Antibacterial activity on stem and leaf of neptunia natans extract / Nur Nazihani Muhamad Nazir
title_fullStr Antibacterial activity on stem and leaf of neptunia natans extract / Nur Nazihani Muhamad Nazir
title_full_unstemmed Antibacterial activity on stem and leaf of neptunia natans extract / Nur Nazihani Muhamad Nazir
title_sort antibacterial activity on stem and leaf of neptunia natans extract / nur nazihani muhamad nazir
publishDate 2018
url http://ir.uitm.edu.my/id/eprint/34325/1/34325.pdf
http://ir.uitm.edu.my/id/eprint/34325/
_version_ 1685651179468488704
score 13.211869

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