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Isolation and optimization of the annealing temperature in PCR for amplification of cytochrome b and 16s rDNA genes of hemibagrus nemurus in Sungai Selangor / Nur Syuhana Zaini

Hemibagrus nemurus also known as Mystus nemurus comes from Bagridae family. This species can be found in wide range of habitats such as rivers and streams, lakes, peat swamps, and marshlands. In this study, the Mystus nemurus was collected in Sungai Selangor. The aims of this study were to amplify t...

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Main Author: Zaini, Nur Syuhana
Format: Student Project
Language:English
Published: 2017
Subjects:
Laboratories. General works
DNA. Deoxyribonucleic acids
Extraction (Chemistry)
Online Access:http://ir.uitm.edu.my/id/eprint/33417/1/PPb_NUR%20SYUHANA%20ZAINI%20AS%20N%2017_5.pdf
http://ir.uitm.edu.my/id/eprint/33417/
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spelling my.uitm.ir.334172020-08-12T09:27:23Z http://ir.uitm.edu.my/id/eprint/33417/ Isolation and optimization of the annealing temperature in PCR for amplification of cytochrome b and 16s rDNA genes of hemibagrus nemurus in Sungai Selangor / Nur Syuhana Zaini Zaini, Nur Syuhana Laboratories. General works DNA. Deoxyribonucleic acids Extraction (Chemistry) Hemibagrus nemurus also known as Mystus nemurus comes from Bagridae family. This species can be found in wide range of habitats such as rivers and streams, lakes, peat swamps, and marshlands. In this study, the Mystus nemurus was collected in Sungai Selangor. The aims of this study were to amplify the 16s rDNA gene and Cytochrome b species of Hemibagrus nemurus in Sungai Selangor and to identify the optimum annealing temperature of PCR for amplification of cytochrome b and 16S rDNA in the Hemibagrus nemurus. Deoxyribonucleic acid extraction of the fish was done by using conventional method. The products of the Deoxyribonucleic acid extraction then were amplified by using polymerase chain reaction with the specific primer for 16s rDNA and cytochrome b. The range of the optimum annealing temperature for this species are 55°C to 58 °C but it shows the better quality product at 56 °C and 58 °C. The product of the Polymerase Chain Reaction was visualized at 500bp which is as the amplicon size referred to the primer characteristics. 2017 Student Project NonPeerReviewed text en http://ir.uitm.edu.my/id/eprint/33417/1/PPb_NUR%20SYUHANA%20ZAINI%20AS%20N%2017_5.pdf Zaini, Nur Syuhana (2017) Isolation and optimization of the annealing temperature in PCR for amplification of cytochrome b and 16s rDNA genes of hemibagrus nemurus in Sungai Selangor / Nur Syuhana Zaini. [Student Project] (Unpublished)
institution Universiti Teknologi Mara
building Tun Abdul Razak Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Teknologi Mara
content_source UiTM Institutional Repository
url_provider http://ir.uitm.edu.my/
language English
topic Laboratories. General works
DNA. Deoxyribonucleic acids
Extraction (Chemistry)
spellingShingle Laboratories. General works
DNA. Deoxyribonucleic acids
Extraction (Chemistry)
Zaini, Nur Syuhana
Isolation and optimization of the annealing temperature in PCR for amplification of cytochrome b and 16s rDNA genes of hemibagrus nemurus in Sungai Selangor / Nur Syuhana Zaini
description Hemibagrus nemurus also known as Mystus nemurus comes from Bagridae family. This species can be found in wide range of habitats such as rivers and streams, lakes, peat swamps, and marshlands. In this study, the Mystus nemurus was collected in Sungai Selangor. The aims of this study were to amplify the 16s rDNA gene and Cytochrome b species of Hemibagrus nemurus in Sungai Selangor and to identify the optimum annealing temperature of PCR for amplification of cytochrome b and 16S rDNA in the Hemibagrus nemurus. Deoxyribonucleic acid extraction of the fish was done by using conventional method. The products of the Deoxyribonucleic acid extraction then were amplified by using polymerase chain reaction with the specific primer for 16s rDNA and cytochrome b. The range of the optimum annealing temperature for this species are 55°C to 58 °C but it shows the better quality product at 56 °C and 58 °C. The product of the Polymerase Chain Reaction was visualized at 500bp which is as the amplicon size referred to the primer characteristics.
format Student Project
author Zaini, Nur Syuhana
author_facet Zaini, Nur Syuhana
author_sort Zaini, Nur Syuhana
title Isolation and optimization of the annealing temperature in PCR for amplification of cytochrome b and 16s rDNA genes of hemibagrus nemurus in Sungai Selangor / Nur Syuhana Zaini
title_short Isolation and optimization of the annealing temperature in PCR for amplification of cytochrome b and 16s rDNA genes of hemibagrus nemurus in Sungai Selangor / Nur Syuhana Zaini
title_full Isolation and optimization of the annealing temperature in PCR for amplification of cytochrome b and 16s rDNA genes of hemibagrus nemurus in Sungai Selangor / Nur Syuhana Zaini
title_fullStr Isolation and optimization of the annealing temperature in PCR for amplification of cytochrome b and 16s rDNA genes of hemibagrus nemurus in Sungai Selangor / Nur Syuhana Zaini
title_full_unstemmed Isolation and optimization of the annealing temperature in PCR for amplification of cytochrome b and 16s rDNA genes of hemibagrus nemurus in Sungai Selangor / Nur Syuhana Zaini
title_sort isolation and optimization of the annealing temperature in pcr for amplification of cytochrome b and 16s rdna genes of hemibagrus nemurus in sungai selangor / nur syuhana zaini
publishDate 2017
url http://ir.uitm.edu.my/id/eprint/33417/1/PPb_NUR%20SYUHANA%20ZAINI%20AS%20N%2017_5.pdf
http://ir.uitm.edu.my/id/eprint/33417/
_version_ 1685651056733716480
score 13.211869

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